Multiplex analysis of antinuclear antibodies by flow cytometry using FIDIS

  • PJ Charles1

    Affiliated with

    Arthritis Res Ther20046(Suppl 1):8

    DOI: 10.1186/ar1050

    Received: 16 January 2004

    Published: 24 February 2004

    Background

    FIDIS (BMD, France) is a multiplex analytical flow cytometry system for the detection of antibodies. The aim of this study was to evaluate the FIDIS connective assay system for the detection of antinuclear antibodies (ANA), and to assess the clinical utility of these parameters in the diagnosis of connective tissue diseases. The FIDIS system simultaneously measures IgG antibodies directed at dsDNA, Ro, La, RNP, Sm, Jo-1, Scl-70, rRNP, and CENP-B.

    Methods

    Sera were obtained from 100 patients with SLE and 100 patients with Sjögren's syndrome. A total of 78 sera from patients with rheumatoid arthritis (RA) and 100 from normal healthy blood donors were also tested.

    Results

    Antibodies to Scl-70, Jo-1, and rRNP were not detected in any of the sera from the disease groups. In the normal control group, the only positive result obtained was in one sera that gave a weak positive result for anti-CENP-B.

    Conclusion

    The FIDIS connective assay system simultaneously measures IgG antibodies directed against nine different specificities. The data presented indicate that this system provides an alternative strategy for the measurement of specific ANA to the use of multiple single analyte assays currently employed in many immunology laboratories.
    Table 1

    Frequency of antibodies detected using FIDIS™

     

    SLE

    SS

    RA

    Anti-Ro

    36

    69

    3.6

    Anti-La

    13

    38

    1.2

    Anti-Sm

    6

    0

    1.2

    Anti-RNP/Sm

    37

    8

    8

    Anti-CENP-B

    2

    2

    0

    Anti-dsDNA

    47

    0

    3.6

    Authors’ Affiliations

    (1)
    Division of Immunology, Hammersmith Hospitals NHS Trust and Kennedy Division, Imperial College

    Copyright

    © BioMed Central Ltd 2004

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