IFN-γ transgenic mice: clues to the pathogenesis of systemic lupus erythematosus?
- John P Seery1
© Current Science Ltd 2000
Received: 22 June 2000
Accepted: 7 August 2000
Published: 25 August 2000
Transgenic mice overexpressing IFN-γ in the epidermis develop an inflammatory skin disease resembling cutaneous lupus erythematosus shortly after birth. By 3 months of age, most female transgenics develop a lupus-like syndrome characterised by production of IgG anti-dsDNA, antihistone and antinucleosome autoantibodies. The autoantibodies are nephritogenic, with one-third of females developing a severe immune complex mediated glomerulonephritis. Analysis of these transgenics suggests that pathogenic autoantibodies arise via an antigen-driven T-cell-dependent mechanism with apoptotic keratinocytes acting as a potential source of autoantigen. The mechanism of autoantibody production in IFN-γ transgenics may be relevant to human lupus and is consistent with a central role for cutaneous T cells in the pathogenesis of systemic lupus erythematosus in man.
Keywordsinterferon-γ lupus skin T cell transgenic mice
Epidemiological evidence implies that systemic lupus erythematosus (SLE) is caused by the action of an environmental agent (or agents) on genetically susceptible individuals . Although the nature of these environmental factors remains unknown, the `downstream' mechanisms involved in pathogenic autoantibody production are being defined. The evidence from both patients and animal models of the disease strongly suggests that antinuclear antibodies (ANA) arise via a classical T-cell-dependent immune response . Furthermore, circumstantial evidence implies that the autoantigens driving this response are nucleoprotein complexes released during apoptosis . The ANA produced are largely responsible for the widespread tissue damage characteristic of the syndrome .
The essential role of IFN-γ in the pathogenesis of immune complex mediated end organ disease has been firmly established in several experimental models . However, there is some evidence that IFN-γ may also play a role earlier in the disease process, specifically in the initiation of ANA production. For example, the emergence of SLE, with de novo anti-dsDNA antibody production, has been described in some patients receiving systemic treatment with IFN-γ, suggesting that excess amounts of the cytokine in susceptible individuals can trigger an auto-immune response relevant to SLE . This contention is supported by results from transgenic mice expressing IFN-γ under the control of the involucrin promoter .
IFN-gamma transgenic mice develop a lupus-like syndrome
Involucrin is a component of the keratinocyte cell envelope and its expression is essentially limited to the suprabasal layers of stratified squamous epithelia . In order to study the role of IFN-γ in the pathogenesis of inflammatory skin disease, we generated transgenic mice expressing the cytokine under the influence of the involucrin promoter . Transgenics have markedly elevated levels of IFN-γ protein in the skin and, shortly after birth, develop an inflammatory skin lesion characterised histologically by a dermal mononuclear infiltrate . Most female transgenics over 3 months of age also produce high levels of IgG antinucleosome, antihistone and anti-dsDNA antibodies. The autoantibodies are nephritogenic, with at least one-third of females developing a severe immune complex mediated glomerulonephritis. Autoantibodies against extractable nuclear antigens are rarer but are present in some female animals. Male IFN-γ transgenics do not develop the lupus phenotype .
IFN-γ has been overexpressed in several extracutaneous tissues, in all cases resulting in the development of only organ specific autoimmunity [10,11]. Taken with our findings, this implies that the skin immune system (SIS) in females has specific properties that, under the wrong circumstances, can trigger the lupus autoimmune response. How does the SIS generate ANA?
The mechanism of ANA production in IFN-gamma transgenic mice
By generating IFN-γ transgenic mice congenitally deficient in αβ T cells, we showed that autoantibody production and end organ damage in this model are completely dependent on the presence of functional αβ T cells , strongly implying that, as in the human disease, the ANA of IFN-γ transgenics arise via a classical antigen-driven T-cell-dependent immune response. What is the source of self-nuclear antigen in these animals and how is it presented to autoreactive T cells?
IFN-γ is a potent stimulus of keratinocyte apoptosis, and both the basal layer of the epidermis and the dermis of IFN-γ transgenics contain large amounts of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling positive material . This material is a likely source of autoantigen in these animals as the self-antigens of SLE are known to cluster in the surface blebs of apoptotic keratinocytes . IFN-γ can stimulate apoptosis by a number of mechanisms; facilitation of Fas/FasL interactions, however, appears to be of particular relevance in keratinocytes . IFN-γ upregulates keratinocyte Fas expression and cells in the epidermal basal layer of IFN-γ transgenics strongly express Fas (Seery et al, unpublished observations). Furthermore, immunocytes expressing FasL are present in the dermal infiltrate (Seery et al, unpublished observations). It is therefore possible that IFN-γ facilitates a productive interaction between these ligands leading to keratinocyte apoptosis. The fact that epidermal apoptosis in these animals is sensitive to inhibition with the pan-caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-(beta-o-methyl)-fluoromethylketone (Seery et al, unpublished observations) supports this concept as Fas independent mechanisms of IFN-γ induced apoptosis are augmented by this agent .
Histological examination of the skin of IFN-γ transgenics suggests two possible mechanisms by which apoptotic material may be presented to autoreactive T cells. First, basal cells in IFN-γ transgenics strongly express major histocompatibility antigen (MHC) class II and intracellular adhesion molecule (ICAM) , and there is evidence that under some circumstances keratinocytes may act as antigen presenting cells (APC) [16,17]. We could not, however, demonstrate B7 expression in the epidermis of these animals and, in the absence of such costimulatory molecules, presentation of antigen by keratinocytes may be tolerogenic (vide infra) . An alternative mechanism of autoreactive T-cell activation is suggested by analysis of epidermal dendritic cell (DC) distribution in IFN-γ transgenics. There is evidence that DCs migrate from inflamed skin to the draining lymph nodes in these animals . Furthermore, remnants of apoptotic cells are known to be taken up by DCs . It seems likely that apoptotic material carried from the skin by these professional APC is presented to autoreactive T cells in the lymph nodes draining the skin, with subsequent activation of B-cell ANA production .
IFN-gamma in human SLE: a central role for cutaneous T cells?
Transgenic models of human disease have a number of limitations. Tissues generally have a limited number of ways in which they respond to insult. Several different types of noxious agents may give rise to a similar pathological picture without necessarily bearing an aetiological relationship to the naturally occurring human disease. However, data from IFN-γ transgenic mice suggest that the SIS is at least capable of playing the central role in SLE pathogenesis. The fact that the lupus-like syndrome in these animals mirrors the human disease in arising via a classical T-cell-dependent mechanism may make it a useful model in which to study specific immunomodulatory therapies.
I thank Dr JM Carroll, Dr EC Wang, Dr V Cattell and Dr FM Watt for interesting discussions and ideas during the course of this work.
- Kaplan D: The onset of disease in twins and siblings with systemic lupus erythematosus. J Rheumatol. 1984, 11: 648-652.PubMedGoogle Scholar
- Radic MZ, Weigert M: Genetic and structural evidence for antigen selection of anti-DNA antibodies. Ann Rev Immunol. 1994, 12: 487-520.View ArticleGoogle Scholar
- Walport MJ: Lupus, DNase and defective disposal of cellular debris. Nat Genet. 2000, 25: 135-136. 10.1038/75963.PubMedView ArticleGoogle Scholar
- Berden JH: Lupus nephritis. Kidney Int. 1997, 52: 538-558.PubMedView ArticleGoogle Scholar
- Kuroiwa T, Lee EG: Cellular interactions in the pathogenesis of lupus nephritis: the role of T cells and macrophages in the amplification of the inflammatory process in the kidney. Lupus . 1998, 7: 597-603.PubMedView ArticleGoogle Scholar
- Graninger WB, Hassfeld W, Pesau BB, Machold KP, Zielinski CC, Smolen JS: Induction of systemic lupus erythemtosus by interferon-gamma in a patient with rheumatoid arthritis. J Rheumatol. 1991, 18: 1621-1622.PubMedGoogle Scholar
- Carroll JM, Crompton T, Seery JP, Watt FM: Transgenic mice expressing IFN-γ in the epidermis have eczema, hair hypopigmentation and hair loss. J Invest Dermatol. 1997, 108: 412-422.PubMedView ArticleGoogle Scholar
- Carroll JM, Albers KM, Garlick JA, Harrington R, Taichman LB: Tissue-and stratum-specific expression of the human involucrin promoter in transgenic mice. Proc Natl Acad Sci USA. 1993, 90: 10270-10274.PubMedPubMed CentralView ArticleGoogle Scholar
- Seery JP, Carroll JM, Cattell V, Watt FM: Antinuclear autoantibodies and lupus nephritis in transgenic mice expressing interferon γ in the epidermis. J Exp Med. 1997, 186: 1451-1459.PubMedPubMed CentralView ArticleGoogle Scholar
- Sarvetnick N, Shizuru J, Liggitt D, Martin L, McIntyre B, Gregory A, Parslow T, Stewart T: Loss of pancreatic islet tolerance induced by beta-cell expression of interferon-gamma. Nature. 1990, 346: 844-847.PubMedView ArticleGoogle Scholar
- Gu D, Wogensen L, Calcutt NA, Xia C, Zhu S, Merlie JP, Fox HS, Lindstrom J, Powell HC, Sarvetnick N: Myasthenia gravis-like syndrome induced by expression of interferon γ in the neuromuscular junction. J Exp Med. 1995, 181: 547-557.PubMedView ArticleGoogle Scholar
- Seery JP, Wang EC, Cattell V, Carroll JM, Owen MJ, Watt FM: A central role for alpha beta T cells in the pathogenesis of murine lupus. J Immunol. 1999, 162: 7241-7248.PubMedGoogle Scholar
- Casciola-Rosen LA, Anhalt G, Rosen A: Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes. J Exp Med. 1994, 179: 1317-1330.PubMedView ArticleGoogle Scholar
- Sayama K, Yonehara S, Watanabe Y, Miki Y: Expression of Fas antigen on keratinocytes in vivo and induction of apoptosis in cultured keratinocytes. J Invest Dermatol. 1994, 103: 330-334.PubMedView ArticleGoogle Scholar
- Quignon F, De Bels F, Koken M, Feunteun J, Ameisen JC, de The H: PML induces a novel caspase-independent death process. Nat Genet. 1998, 20: 259-265. 10.1038/3068.PubMedView ArticleGoogle Scholar
- Gaspari AA, Katz SI: Induction and functional characterization of class II MHC (Ia) antigens on murine keratinocytes. J Immunol. 1988, 140: 2956-2963.PubMedGoogle Scholar
- Bal V, McIndoe A, Denton G, Hudson D, Lombardi G, Lamb J, Lechler R: Antigen presentation by keratinocytes induces tolerance in human T cells. Eur J Immunol. 1990, 20: 1893-1897.PubMedView ArticleGoogle Scholar
- Nakajima M, Nakajima A, Kayagaki N, Honda M, Yagita H, Okumura K: Expression of Fas ligand and its receptor in cutaneous lupus: implication in tissue injury. Clin Immunol Immunopathol . 1997, 83: 223-229. 10.1006/clin.1997.4352.PubMedView ArticleGoogle Scholar
- Bennion SD, Middleton MH, David-Bajar KM, Brice S, Norris DA: In three types of interface dermatitis, different patterns of expression of intercellular adhesion molecule-1 (ICAM-1) indicate different triggers of disease. J Invest Dermatol. 1995, 105 (suppl 1): 71S-79S.View ArticleGoogle Scholar
- Kind P, Lehmann P, Plewig G: Phototesting in lupus erythematosus. J Invest Dermatol. 1993, 100: 53S-57S.PubMedView ArticleGoogle Scholar
- Pablos JL, Santiago B, Galindo M, Carreira PE, Ballestin C, Gomez-Reino JJ: Keratinocyte apoptosis and p53 expression in cutaneous lupus and dermatomyositis. J Pathol. 1999, 188: 63-68. 10.1002/(SICI)1096-9896(199905)188:1<63::AID-PATH303>3.0.CO;2-E.PubMedView ArticleGoogle Scholar
- Velthuis PJ, van Weelden H, van Wichen D, Baart de la Faille H: Immunohistopathology of light-induced skin lesions in lupus erythematosus. Acta Derm Venereol. 1990, 70: 93-98.PubMedGoogle Scholar
- Prinz JC, Meurer M, Reiter C, Rieber EP, Plewig G, Riethmuller G: Treatment of severe cutaneous lupus erythematosus with a chimeric CD4 monoclonal antibody, cM-T412. J Am Acad Dermatol. 1996, 34 (2 part 1): 244-252.View ArticleGoogle Scholar
- Robert C, Kupper TS: Inflammatory skin diseases, T cells, and immune surveillance. N Engl J Med. 1999, 24: 1817-1828.Google Scholar
- McGrath H, Martinez-Osuna P, Lee FA: Ultraviolet-A1 (340-400 nm) irradiation therapy in systemic lupus erythematosus. Lupus. 1996, 5: 269-274.PubMedView ArticleGoogle Scholar
- Casciola-Rosen L, Rosen A: Ultraviolet light-induced keratinocyte apoptosis:a potential mechanism for the induction of skin lesions and autoantibody production in LE. Lupus. 1997, 6: 175-180.PubMedView ArticleGoogle Scholar
- Venables PJ: Diagnosis and treatment of systemic lupus erythematosus. BMJ. 1993, 307: 663-666.PubMedPubMed CentralView ArticleGoogle Scholar
- Ansel JC, Mountz J, Steinberg AD, DeFabo E, Green I: Effects of UV radiation on autoimmune strains of mice:increased mortality and accelerated autoimmunity in BXSB mice. J Invest Dermatol. 1985, 85: 181-186.PubMedView ArticleGoogle Scholar
- Nickoloff BJ, Turka LA: Immunological functions of non-professional antigen-presenting cells: new insights from studies of T-cell interactions with keratinocytes. Immunol Today. 1994, 15: 464-469.PubMedView ArticleGoogle Scholar