Volume 8 Supplement 2
Interleukin-6: discovery of a pleiotropic cytokine
© BioMed Central Ltd 2006
Published: 28 July 2006
In the late 1960s, the essential role played by T cells in antibody production was reported. This led to our hypothesis that certain molecules would have to be released from T cells to effect the stimulation of B cells. This hypothesis was shown to be true. There were certain factors present in the culture supernatant of T cells that induced proliferation and differentiation of B cells. The factor that induced B cells to produce immunoglobulins was initially named B cell stimulatory factor-2. The cDNA encoding the human B cell stimulatory factor-2 was cloned in 1986. At the same time, IFN-β2 and a 26 kDa protein in the fibroblasts were independently cloned and found to be identical to B cell stimulatory factor-2. Later, a hybridoma/plasmacytoma growth factor and a hepatocyte stimulating factor were also proven to be the same molecule as B cell stimulatory factor-2. Various names were used for this single molecule because of its multiple biological activities, but these have all been unified and the molecule is now known as IL-6. Since the discovery of IL-6, rapid progress has been made in our understanding of IL-6 activities, the IL-6 receptor system and the IL-6 signal transduction mechanism. More importantly, it has been shown to be involved in a number of diseases such as rheumatoid arthritis and Castleman's disease. When taking into account all the accumulated basic research on the various aspects of this molecule, it appeared that blocking the activity of IL-6 was a feasible, new therapeutic approach for chronic inflammatory diseases.
A selection of published research resulting in the characterization of factors involved in T/B cell interactions
Discovery of soluble factors enhancing antibody production
Discovery of a T cell replacing factor
Induction of immunoglobulin production in B cells using rabbit lymphocytes or murine B cells
Two factors involved in B cell growth and B cell differentiation
BCGF-II induced growth of and IgM production in murine B cells (now IL-5)
BSF-1 cloned (now IL-4)
Discovery of interleukin-6
In 1986 one of these factors was cloned and studied, and it is now referred to as IL-6. This molecule possesses a wide range of activities, and as a result laboratories used various different names to refer to it, depending on their research interests. It has been termed B cell stimulatory factor-2 and was considered a novel IFN (IFN-β2), but studies with recombinant IL-6 and anti-IL-6 antibody demonstrated that IL-6 had no IFN activity . The molecule exhibited a strong stimulatory effect on growth of murine plasmacytoma  and human myeloma , and was studied under the names hybridoma plasmacytoma growth factor and hepatocyte stimulating factor, which induces acute phase reactions [6, 7]. Moreover, because it induces differentiation of myeloid precursors, it has been referred to as macrophage granulocyte inducer type 2 . In addition, IL-6 plays a key role in inflammation, being the main inducer of C-reactive protein, fibrinogen and serum amyloid A protein, as well as other factors.
The activities described above were confirmed using IL-6 transgenic mouse models, revealing massive stimulation of plasma cells in the spleen and lymph nodes. Transgenic expression of IL-6 in BALB/c mice induced monoclonal transplantable plasmacytoma, showing that IL-6 functions as a plasmacytoma/myeloma growth factor. A study conducted in an IL-6 knockout mouse model  revealed that, compared with wild type, the antiviral antibody response was inhibited by more than 90% following immunization with a vesicular stomatitis virus. In a parallel experiment turpentine injection induced strong inflammation, but, once again, in the IL-6 knockout mice almost 90% of this acute phase response was blocked. These experiments confirm that IL-6 functions as a B cell stimulatory factor to induce antibody production and as a hepatocyte stimulatory factor to induce acute phase reactions.
As well as the functions described above, IL-6 has various other biological activities (exemplified by the numerous abbreviations used to describe IL-6). IL-6 is a pleiotropic cytokine that influences antigen-specific immune responses and inflammatory reactions. It has also been shown to induce T cell growth and cytotoxic T cell differentiation by augmenting IL-2 receptor expression  and IL-2 production . IL-6 also induces proliferation of thymocytes and probably plays a role in the development of thymic T cells. In haematopoiesis, IL-6 acts synergistically with IL-3 to support the formation of multilineage blast cell colonies, and it also induces macrophage differentiation and megakaryocyte differentiation . IL-6 has profound effects on bone and can induce osteoclast differentiation and activation in vitro . It appears to be involved in synovial inflammation and in damage to peri-articular cartilage and bone. A correlation has also been identified between serum IL-6 levels and the severity of radiographic destruction . Furthermore, it has been demonstrated to decrease aggrecan protein and type II collagen production by chondrocytes . IL-6 induces endothelial expression of adhesion molecules and endothelial production of monocyte chemoattractant protein-1 , a chemokine that is responsible for monocyte recruitment. Moreover, IL-6 knockout mice exhibit impaired leucocyte accumulation at the site of inflammation  and defective transition to a chronic mononuclear cell infiltrate . It is also involved in the recruitment of mesenchymal vascular cells and neoangiogenesis in vivo , and has been shown to induce proliferation of synovial fibroblasts . Finally, IL-6 appears to be involved in damage to cartilage and bone .
Interleukin-6 receptor system
Following the cloning of the cDNA of IL-6, the structure and signaling function of the IL-6 receptor was studied. The IL-6 receptor system has an unusual organization. It consists of two polypeptide chains : an 80 kDa IL-6 receptor and a 130 kDa signal transducer (gp130). The 80 kDa receptor exists in two forms, namely the transmembrane form and a soluble form. The transmembrane form has a short intracytoplasmic region and, upon stimulation by binding of the IL-6 molecule, triggers an association with gp130. The soluble receptor can form a stimulatory complex with IL-6, which can associate with gp130 and trigger cellular events called trans-signaling. The gp130 has a transmembrane domain and is responsible for transducing the signal across the membrane. It is worth noting that the gp130 is expressed ubiquitously in tissues  and is used in the signaling of many other members of the IL-6 family of cytokines, including leukaemia inhibitory factor, ciliary neurotrophic factor, oncostatin M, IL-11 and cardiotropin-1 [22–26]. The sharing of the gp130 molecule by other IL-6 superfamily cytokines may explain their functional redundancy. This can also be seen within the other cytokine systems, for example IL-3, IL-5 and granulocyte-macrophage colony-stimulating factor, and the group of interleukins (IL-2, IL-4, IL-7, IL-9 and IL-15); both groups use a common receptor component.
IL-6 and its receptor interact to form a complex consisting of two IL-6 molecules plus two IL-6 receptor proteins and two gp130 proteins. The dimerized gp130 then transduces the signals. It is worth noting that the soluble IL-6 receptor also exhibits an agonistic effect in complex with IL-6 and can couple with the gp130 to effect transduction.
One of the difficulties in understanding the mechanism of action of gp130 arises from the fact that it does not possess a specific catalytic domain. This issue was resolved with the discovery of the Janus activated kinase (JAK)-signal transducer and activator of transcription (STAT) pathway; these catalytic molecules associate with the cytokine receptors . The signaling has now been completely elucidated. Activation of gp130 leads to the activation of the intracytoplasmic JAK tyrosine kinases (Janus family tyrosine kinases). Within minutes of IL-6 stimulation, these kinases cause induction of tyrosine phosphorylation and recruitment of STAT3, which dimerizes and is translocated to the nucleus and leads to gene expression.
IL-6 is essential for regulation of the immune process; however, overproduction of the cytokine leads to inflammation and disease (such as rheumatoid arthritis, systemic juvenile arthritis and Crohn's disease). As a result, the cytokine must be regulated to control both the magnitude and duration of response.
SOCS-1 directly interacts with JAKs and so inhibits their catalytic activity, whereas SOCS-3 inhibits cytokine signaling by binding to the receptor complex. In the IL-6 signal pathway, SOCS-1 deficient mice have an intact IL-6 signaling pathway, suggesting that SOCS-3 may act as a crucial inhibitor of IL-6 signaling in vivo. PIASs constitute a family of constitutively expressed negative regulators of STATs. PIAS-3 associates specifically with activated STAT3 but not with STAT1, resulting in blockade of all STAT3-mediated gene transcription . PIAS-3 is especially well known as an inhibitor of IL-6 signaling in M1 cell lines (a mouse myeloid leukaemia cell line).
Constitutive expression of PIAS proteins implies that their physiological role may differ from that of SOCS proteins, which are transiently induced by cytokine stimulation. However, the differences in physiological role between these two proteins are yet to be established.
Interleukin-6 overproduction and disease
A variety of studies have demonstrated that overproduction of IL-6 contributes to the pathogenesis of various autoimmune and inflammatory diseases. (For a review of IL-6 in auto-immune disease and chronic inflammatory proliferative disease, see the article by Ishihara and Hirano .) That IL-6 is involved in autoimmunity was first implied by the facts that patients with cardiac myxoma frequently exhibit autoimmune symptoms and that cardiac myxoma cells produce IL-6 . Also, synovial fluid from patients with rheumatoid arthritis contain elevated levels of IL-6 .
Since those early findings, several lines of evidence have been reported that suggest the involvement of IL-6 in auto-immune disease, chronic inflammatory proliferative disease and B cell malignancy, as well as Castleman's disease, and plasmacytoma/multiple myeloma . Furthermore, IL-6 is required for experimentally induced autoimmune diseases, including type II collagen-induced arthritis and antigen-induced arthritis [33–35], myelin oligodendrocyte protein-induced experimental autoimmune encephalomyelitis [36, 37], and pristane-induced autoantibody production . These findings, together with accumulating evidence from a large body of clinical studies, suggest that IL-6 dependent signaling pathways are involved in the pathogenesis of experimentally induced autoimmune diseases and of naturally occurring autoimmune diseases. However, how and at what levels IL-6 plays its roles in the complex processes of these diseases remain unknown.
Based on the findings described above, the potentially therapeutic strategy of blocking the IL-6 signaling pathway seemed logical and appealing. One approach that has been tested was inhibition of the IL-6 molecule itself. However, use of mAbs to human IL-6 in the clinical setting has yielded unsatisfactory results. Administration of a mAb against IL-6 leads to persistence and accumulation of the cytokine in the circulation in the form of immune complexes, increasing the half-life of IL-6. This prevents such a single mAb to IL-6 from neutralizing the effects of chronic overproduction of IL-6 . In mice, the use of a mixture of mAbs directed against different epitopes of IL-6 reduced the cytokine carrier effect of a single mAb . It has also been shown that immunization with a modified IL-6 molecule leads to induction of a polyclonal antibody response to IL-6, which is able to neutralize fully the phenotype induced by chronic overproduction of human IL-6 in human IL-6 transgenic mice . These observations suggest that use of antibodies with multiple specificities for IL-6 may be more efficacious than use of a single mAb. However, neither of these approaches is readily applicable to treatment in humans.
Clinical studies have shown the utility of IL-6 receptor blockade in Castleman's disease . Studies have also demonstrated acceptable safety and significant efficacy in trials in both adult rheumatoid arthritis and systemic onset juvenile arthritis [49–52].
Because the effects of anti-IL-6 receptor antibody treatment on the diseases were so dramatic, further studies of immune functions and gene expression in patients before and after treatment might provide important insights into the pathogenesis of these diseases. The following papers further investigate the specific effects of IL-6 biology in animal models and in humans and the effects of blocking the IL-6 receptor.
Janus activated kinase
protein inhibitors of activated STATs
suppressors of cytokine signaling
signal transducer and activator of transcription.
The author thanks Osamu Okuda, PhD for valuable assistance with the preparation of this manuscript.
- Miller JF, Mitchell GF: Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968, 128: 801-820. 10.1084/jem.128.4.801.PubMed CentralView ArticlePubMedGoogle Scholar
- Claman HN, Chaperon EA, Selner JC: Thymusmarrow immunocompetence. 3. The requirement for living thymus cells. Proc Soc Exp Biol Med. 1968, 127: 462-466.View ArticlePubMedGoogle Scholar
- Hirano T, Matsuda T, Hosoi K, Okano A, Matsui H, Kishimoto T: Absence of antiviral activity in recombinant B cell stimulatory factor 2 (BSF-2). Immunol Lett. 1988, 17: 41-45. 10.1016/0165-2478(88)90099-5.View ArticlePubMedGoogle Scholar
- Suematsu S, Matsusaka T, Matsuda T, Ohno S, Miyazaki J, Yamamura K, Hirano T, Kishimoto T: Generation of plasmacytomas with the chromosomal translocation t(12;15) in interleukin 6 transgenic mice. Proc Natl Acad Sci USA. 1992, 89: 232-235. 10.1073/pnas.89.1.232.PubMed CentralView ArticlePubMedGoogle Scholar
- Kawano M, Hirano T, Matsuda T, Taga T, Horii Y, Iwato K, Asaoku H, Tang B, Tanabe O, Tanaka H, et al: Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature. 1988, 332: 83-85. 10.1038/332083a0.View ArticlePubMedGoogle Scholar
- Gauldie J, Richards C, Harnish D, Lansdorp P, Baumann H: Interferon β2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells. Proc Natl Acad Sci USA. 1987, 84: 7251-7255. 10.1073/pnas.84.20.7251.PubMed CentralView ArticlePubMedGoogle Scholar
- Andus T, Geiger T, Hirano T, Northoff H, Ganter U, Bauer J, Kishimoto T, Heinrich PC: Recombinant human B cell stimulatory factor 2 (BSF-2/IFN-β2) regulates β-fibrinogen and albumin mRNA levels in Fao-9 cells. FEBS Lett. 1987, 221: 18-22. 10.1016/0014-5793(87)80344-7.View ArticlePubMedGoogle Scholar
- Shabo Y, Lotem J, Rubinstein M, Revel M, Clark SC, Wolf SF, Kamen R, Sachs L: The myeloid blood cell differentiation-inducing protein MGI-2A is interleukin-6. Blood. 1988, 72: 2070-2073.PubMedGoogle Scholar
- Kopf M, Baumann H, Freer G, Freudenberg M, Lamers M, Kishimoto T, Zinkernagel R, Bluethmann H, Kohler G: Impaired immune and acute-phase responses in interleukin-6-deficient mice. Nature. 1994, 368: 339-342. 10.1038/368339a0.View ArticlePubMedGoogle Scholar
- Noma Y, Sideras P, Naito T, Bergstedt-Lindquist S, Azuma C, Severinson E, Tanabe T, Kinashi T, Matsuda F, Yaoita Y, et al: Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter. Nature. 1986, 319: 640-646. 10.1038/319640a0.View ArticlePubMedGoogle Scholar
- Hibi M, Murakami M, Saito M, Hirano T, Taga T, Kishimoto T: Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 1990, 63: 1149-1157. 10.1016/0092-8674(90)90411-7.View ArticlePubMedGoogle Scholar
- Yoshizaki K, Nishimoto N, Mihara M, Kishimoto T: Therapy of rheumatoid arthritis by blocking IL-6 signal transduction with a humanized anti-IL-6 receptor antibody. Springer Semin Immunopathol. 1998, 20: 247-59.View ArticlePubMedGoogle Scholar
- Tamura T, Udagawa N, Takahashi N, Miyaura C, Tanaka S, Yamada Y, Koishihara Y, Ohsugi Y, Kumaki K, Taga T, et al: Soluble interleukin-6 receptor triggers osteoclast formation by interleukin 6. Proc Natl Acad Sci USA. 1993, 90: 11924-11928. 10.1073/pnas.90.24.11924.PubMed CentralView ArticlePubMedGoogle Scholar
- Dasgupta B, Corkill M, Kirkham B, Gibson T, Panayi G: Serial estimation of interleukin 6 as a measure of systemic disease in rheumatoid arthritis. J Rheumatol. 1992, 19: 22-25.PubMedGoogle Scholar
- Legendre F, Dudhia J, Pujol JP, Bogdanowicz P: JAK/STAT but not ERK1/ERK2 pathway mediates interleukin (IL)-6/soluble IL-6R down-regulation of Type II collagen, aggrecan core, and link protein transcription in articular chondrocytes. Association with a down-regulation of SOX9 expression. J Biol Chem. 2003, 278: 2903-2912. 10.1074/jbc.M110773200.View ArticlePubMedGoogle Scholar
- Romano M, Sironi M, Toniatti C, Polentarutti N, Fruscella P, Ghezzi P, Faggioni R, Luini W, van Hinsbergh V, Sozzani S, et al: Role of IL-6 and its soluble receptor in induction of chemokines and leukocyte recruitment. Immunity. 1997, 6: 315-325. 10.1016/S1074-7613(00)80334-9.View ArticlePubMedGoogle Scholar
- Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA: IL-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Immunity. 2001, 14: 705-714. 10.1016/S1074-7613(01)00151-0.View ArticlePubMedGoogle Scholar
- Giraudo E, Arese M, Toniatti C, Strasly M, Primo L, Mantovani A, Ciliberto G, Bussolini F: IL-6 is an in vitro and in vivo autocrine growth factor for middle T antigen-transformed endothelial cells. J Immunol. 1996, 157: 2618-2623.PubMedGoogle Scholar
- Mihara M, Moriya Y, Kishimoto T, Ohsugi Y: Interleukin-6 (IL-6) induces the proliferation of synovial fibroblastic cells in the presence of soluble IL-6 receptor. Br J Rheumatol. 1995, 34: 321-325.View ArticlePubMedGoogle Scholar
- Kudo O, Sabokbar A, Pocock A, Itonaga I, Fujikawa Y, Athanasou NA: Interleukin-6 and interleukin-11 support human osteoclast formation by a RANKL-independent mechanism. Bone. 2003, 32: 1-7. 10.1016/S8756-3282(02)00915-8.View ArticlePubMedGoogle Scholar
- Taga T, Hibi M, Hirata Y, Yamasaki K, Yasukawa K, Matsuda T, Hirano T, Kishimoto T: Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130. Cell. 1989, 58: 573-581. 10.1016/0092-8674(89)90438-8.View ArticlePubMedGoogle Scholar
- Ip NY, Nye SH, Boulton TG, Davis S, Taga T, Li Y, Birren SJ, Yasukawa K, Kishimoto T, Anderson DJ, et al: CNTF and LIF act on neuronal cells via shared signaling pathways that involve the IL-6 signal transducing receptor component gp130. Cell. 1992, 69: 1121-1132. 10.1016/0092-8674(92)90634-O.View ArticlePubMedGoogle Scholar
- Gearing DP, Comeau MR, Friend DJ, Gimpel SD, Thut CJ, McGourty J, Brasher KK, King JA, Gillis S, Mosley B, et al: The IL-6 signal transducer, gp130: an oncostatin M receptor and affinity converter for the LIF receptor. Science. 1992, 255: 1434-1437.View ArticlePubMedGoogle Scholar
- Liu J, Modrell B, Aruffo A, Marken JS, Taga T, Yasukawa K, Murakami M, Kishimoto T, Shoyab M: Interleukin-6 signal transducer gp130 mediates oncostatin M signaling. J Biol Chem. 1992, 267: 16763-16766.PubMedGoogle Scholar
- Yin T, Taga T, Tsang ML, Yasukawa K, Kishimoto T, Yang YC: Involvement of IL-6 signal transducer gp130 in IL-11-mediated signal transduction. J Immunol. 1993, 151: 2555-2561.PubMedGoogle Scholar
- Pennica D, Shaw KJ, Swanson TA, Moore MW, Shelton DL, Zioncheck KA, Rosenthal A, Taga T, Paoni NF, Wood WI: Cardiotrophin-1. Biological activities and binding to the leukemia inhibitory factor receptor/gp130 signaling complex. J Biol Chem. 1995, 270: 10915-10922. 10.1074/jbc.270.18.10915.View ArticlePubMedGoogle Scholar
- Darnell JE, Kerr IM, Stark GR: Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science. 1994, 264: 1415-1421.View ArticlePubMedGoogle Scholar
- Chung CD, Liao J, Lui B, Rao X, Jay P, Berta P, Shuai K: Specific inhibiton of Stat3 signal transduction by PIAS3. Science. 1997, 278: 1803-1805. 10.1126/science.278.5344.1803.View ArticlePubMedGoogle Scholar
- Ishihara K, Hirano T: IL-6 in autoimmune disease and chronic inflammatory proliferative disease. Cytokine Growth Factor Rev. 2002, 13: 357-368. 10.1016/S1359-6101(02)00027-8.View ArticlePubMedGoogle Scholar
- Hirano T, Taga T, Yasukawa K, Nakajima K, Nakano N, Takatsuki F, Shimizu M, Murashima A, Tsunasawa S, Sakiyama F, et al: Human B-cell differentiation factor defined by an anti-peptide antibody and its possible role in autoantibody production. Proc Natl Acad Sci USA. 1987, 84: 228-231. 10.1073/pnas.84.1.228.PubMed CentralView ArticlePubMedGoogle Scholar
- Hirano T, Matsuda T, Turner M, Miyasaka N, Buchan G, Tang B, Sato K, Shimizu M, Maini R, Feldmann M, et al: Excessive production of interleukin 6/B cell stimulatory factor-2 in rheumatoid arthritis. Eur J Immunol. 1988, 18: 1797-1801.View ArticlePubMedGoogle Scholar
- Hirano T: Interleukin 6 and its receptor: ten years later. Int Rev Immunol. 1998, 16: 249-284.View ArticlePubMedGoogle Scholar
- Alonzi T, Fattori E, Lazzaro D, Costa P, Probert L, Kollias G, De Benedetti F, Poli V, Ciliberto G: Interleukin 6 is required for the development of collagen-induced arthritis. J Exp Med. 1998, 187: 461-468. 10.1084/jem.187.4.461.PubMed CentralView ArticlePubMedGoogle Scholar
- Sasai M, Saeki Y, Ohshima S, Nishioka K, Mima T, Tanaka T, Katada Y, Yoshizaki K, Suemura M, Kishimoto T, et al: Delayed onset and reduced severity of collagen-induced arthritis in interleukin-6-deficient mice. Arthritis Rheum. 1999, 42: 1635-1643. 10.1002/1529-0131(199908)42:8<1635::AID-ANR11>3.0.CO;2-Q.View ArticlePubMedGoogle Scholar
- Ohshima S, Saeki Y, Mima T, Sasai M, Nishioka K, Nomura S, Kopf M, Katada Y, Tanaka T, Suemura M, Kishimoto T: Interleukin 6 plays a key role in the development of antigen-induced arthritis. Proc Natl Acad Sci USA. 1998, 95: 8222-8226. 10.1073/pnas.95.14.8222.PubMed CentralView ArticlePubMedGoogle Scholar
- Eugster HP, Frei K, Kopf M, Lassmann H, Fontana A: IL-6-deficient mice resist myelin oligodendrocyte glycoprotein-induced autoimmune encephalomyelitis. Eur J Immunol. 1998, 28: 2178-2187. 10.1002/(SICI)1521-4141(199807)28:07<2178::AID-IMMU2178>3.0.CO;2-D.View ArticlePubMedGoogle Scholar
- Samoilova EB, Horton JL, Hilliard B, Liu TS, Chen Y: IL-6-deficient mice are resistant to experimental autoimmune encephalomyelitis: roles of IL-6 in the activation and differentiation of autoreactive T cells. J Immunol. 1998, 161: 6480-6486.PubMedGoogle Scholar
- Richards HB, Satoh M, Jennette JC, Okano T, Kanwar YS, Reeves WH: Disparate T cell requirements of two subsets of lupus-specific autoantibodies in pristane-treated mice. Clin Exp Immunol. 1999, 115: 547-553. 10.1046/j.1365-2249.1999.00825.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Lu ZY, Brailly H, Wijdenes J, Bataille R, Rossi JF, Klein B: Measurement of whole body interleukin-6 (IL-6) production: prediction of the efficacy of anti-IL-6 treatments. Blood. 1995, 86: 3123-3131.PubMedGoogle Scholar
- Montero-Julian FA, Gautherot E, Wijdenes J, Klein B, Brailly H: Pharmacokinetics of interleukin-6 during therapy with anti-interleukin-6 monoclonal antibodies: enhanced clearance of interleukin-6 by a combination of three anti-interleukin-6 antibodies. J Interferon Res. 1994, 14: 301-302.View ArticlePubMedGoogle Scholar
- De Benedetti F, Pignatti P, Vivarelli M, Meazza C, Ciliberto G, Savino R, Martini A: In vivo neutralization of human IL-6 (hIL-6) achieved by immunization of hIL-6-transgenic mice with a hIL-6 receptor antagonist. J Immunol. 2001, 166: 4334-4340.View ArticlePubMedGoogle Scholar
- Ding C, Jones G: Technology evaluation: MRA, Chugai. Curr Opin Mol Ther. 2003, 5: 64-69.PubMedGoogle Scholar
- Sato K, Tsuchiya M, Saldanha J, Koishihara Y, Ohsugi Y, Kishimoto T, Bendig MM: Reshaping a human antibody to inhibit the interleukin 6-dependent tumor cell growth. Cancer Res. 1993, 53: 851-856.PubMedGoogle Scholar
- Kotake S, Sato K, Kim KJ, Takahashi N, Udagawa N, Nakamura I, Yamaguchi A, Kishimoto T, Suda T, Kashiwazaki S: Interleukin-6 and soluble interleukin-6 receptors in the synovial fluids from rheumatoid arthritis patients are responsible for osteoclastlike cell formation. J Bone Miner Res. 1996, 11: 88View ArticlePubMedGoogle Scholar
- Mihara M, Moriya Y, Kishimoto T, Oshugi Y: Interleukin-6 (IL-6) induces the proliferation of synovial fibroblastic cells in the presence of soluble IL-6 receptor. Br J Rheumatol. 1995, 34: 321View ArticlePubMedGoogle Scholar
- Franchimont N, Rydziel S, Delaney AM, Canalis E: Interleukin-6 and its soluble receptor cause a marked induction of collagenase 3 expression in rat osteoblastic cultures. J Biol Chem. 1997, 272: 12144-10.1074/jbc.272.18.12144.View ArticlePubMedGoogle Scholar
- Flannery CR, Little CB, Hughes CE, Curtis CL, Caterson B, Jones SA: IL-6 and its soluble receptor augment aggrecanase-mediated proteoglycan catabolism in articular cartilage. Matrix Biol. 2000, 19: 549-10.1016/S0945-053X(00)00111-6.View ArticlePubMedGoogle Scholar
- Nishimoto N, Kanakura Y, Aozasa K, Johkoh T, Nakamura M, Nakano S, Nakano N, Ikeda Y, Sasaki T, Nishioka K, et al: Humanized anti-interleukin-6 receptor antibody treatment of multicentric Castleman disease. Blood. 2005, 106: 2627-2632. 10.1182/blood-2004-12-4602.View ArticlePubMedGoogle Scholar
- Choy EH, Isenberg DA, Garrood T, Farrow S, Ioannou Y, Bird H, Cheung N, Williams B, Hazleman B, Price R, et al: Therapeutic benefit of blocking interleukin-6 activity with an anti-interleukin-6 receptor monoclonal antibody in rheumatoid arthritis: a randomized, double-blind, placebo-controlled, dose-escalation trial. Arthritis Rheum. 2002, 46: 3143-3150. 10.1002/art.10623.View ArticlePubMedGoogle Scholar
- Nishimoto N, Yoshizaki K, Miyasaka N, Yamamoto K, Kawai S, Takeuchi T, Hashimoto J, Kishimoto T: Long-term safety and efficacy of anti-interleukin 6 receptor antibody (MRA) in patients with rheumatoid arthritis [abstract]. Presented at Annual Scientific Meeting of the American College of Rheumatology. Orlando: Wiley Interscience, S126-23–28 October 2003
- Nishimoto N, Yoshizaki K, Miyasaka N, Yamamoto K, Kawai S, Takeuchi T, Hashimoto J, Azuma J, Kishimoto T: Treatment of rheumatoid arthritis with humanized anti-interleukin-6 receptor antibody: a multicenter, double-blind, placebo-controlled trial. Arthritis Rheum. 2004, 50: 1761-1769. 10.1002/art.20303.View ArticlePubMedGoogle Scholar
- Yokota S, Miyamae T, Imagawa T, Iwata N, Katakura S, Mori M, Woo P, Nishimoto N, Yoshizaki K, Kishimoto T: Therapeutic efficacy of humanized anti-IL-6 receptor antibody for children with systemic-onset juvenile idiopathic arthritis. Arthritis Rheum. 2005, 52: 818-825. 10.1002/art.20944.View ArticlePubMedGoogle Scholar
- Kishimoto T, Ishizaka K: Regulation of antibody response in vitro. VII. Enhancing soluble factors for IgG and IgE antibody response. J Immunol. 1973, 111: 1194-1205.PubMedGoogle Scholar
- Kishimoto T, Ishizaka K: Regulation of antibody response in vitro. VI. Carrier-specific helper cells for IgG and IgE antibody response. J Immunol. 1973, 111: 720-732.PubMedGoogle Scholar
- Kishimoto T, Ishizaka K: Regulation of antibody response in vitro. V. Effect of carrier-specific helper cells on generation of hapten-specific memory cells of different immunoglobulin classes. J Immunol. 1973, 111: 1-9.PubMedGoogle Scholar
- Schimpl A, Wecker E: Studies on the source and action of the T-cell replacing factor (TRF). Adv Exp Med Biol. 1973, 29: 179-182.View ArticlePubMedGoogle Scholar
- Kishimoto T, Miyake T, Nishizawa Y, Watanabe T, Yamamura Y: Triggering mechanism of B lymphocytes. I. Effect of anti-immunoglobulin and enhancing soluble factor on differentiation and proliferation of B cells. J Immunol. 1975, 115: 1179-1184.PubMedGoogle Scholar
- Parker DC, Wadsworth DC, Schneider GB: Activation of murine B lymphocytes by anti-immunoglobulin is an inductive signal leading to immunoglobulin secretion. J Exp Med. 1980, 152: 138-150. 10.1084/jem.152.1.138.View ArticlePubMedGoogle Scholar
- Kishimoto T, Hirano T, Kuritani T, Yamamura Y, Ralph P, Good RA: Induction of IgG production in human B lymphoblastoid cell lines with normal human T cells. Nature. 1978, 271: 756-758. 10.1038/271756a0.View ArticlePubMedGoogle Scholar
- Howard M, Farrar J, Hilfiker M, Johnson B, Takatsu K, Hamaoka T, Paul WE: Identification of a T cell-derived B cell growth factor distinct from interleukin 2. J Exp Med. 1982, 155: 914-923. 10.1084/jem.155.3.914.View ArticlePubMedGoogle Scholar
- Kinashi T, Harada N, Severinson E, Tanabe T, Sideras P, Konishi M, Azuma C, Tominaga A, Bergstedt-Lindqvist S, Takahashi M, et al: Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II. Nature. 1986, 324: 70-73. 10.1038/324070a0.View ArticlePubMedGoogle Scholar
- Hirano T, Yasukawa K, Harada H, Taga T, Watanabe Y, Matsuda T, Kashiwamura S, Nakajima K, Koyama K, Iwamatsu A, et al: Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature. 1986, 324: 73-76. 10.1038/324073a0.View ArticlePubMedGoogle Scholar