Volume 3 Supplement 2

21st European Workshop for Rheumatology Research

Open Access

Temporal expression of cytokines and chemokines in rat adjuvant-induced arthritis

  • Z Szekanecz1, 2,
  • MM Halloran1, 2,
  • JM Woods1, 2,
  • MV Volin1, 2,
  • GK Haines1, 2 and
  • AE Koch1, 2
Arthritis Research & Therapy20013(Suppl 2):P024

DOI: 10.1186/ar193

Received: 15 January 2001

Published: 26 January 2001

Adjuvant-induced arthritis (AIA) in rats is a relevant model for human rheumatoid arthritis (RA). In this study, th expression of the cytokines TNF-alpha, IL-1 and IL-6, as well as the chemokines MIP-1-alpha, MCP-1 and ENA-78 in the sera and joint homogenates of AIA and control, sham-injected rats was studied over a 47-day period. All of these cytokines and chemokines showed increased production in AIA. In addition, TNF-alpha, IL-1, ENA-78 and MIP-1-alpha could be termed as "early" mediators, as their production increased in the first 14-21 days and it correlated with early events in synovitis, such as neutrophil ingress, joint swelling and general symptoms. TNF-alpha may have mostly systemic, while IL-1 mainly local synovial effects. IL-6 and MCP-1 were found to be "late" inflammatory mediators, as their secretion was up-regulated after 2 weeks post-adjuvant injection and remained high during the observation period. Also, significant correlation was found between the production of TNF-alpha and that of chemokines. In conclusion, the differential expression of "early" and "late" cytokines and chemokines may account for various events underlying synovitis in AIA.

Authors’ Affiliations

(1)
Third Department of Medicine, University of Debrecen
(2)
Northwestern University

Copyright

© BioMed Central Ltd 2001

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