Inhibitory effects of ZSTK474, a novel phosphoinositide 3-kinase inhibitor, on osteoclasts and collagen-induced arthritis in mice
© Toyama et al.; licensee BioMed Central Ltd. 2010
Received: 30 November 2009
Accepted: 18 May 2010
Published: 18 May 2010
Targeting joint destruction induced by osteoclasts (OCs) is critical for management of patients with rheumatoid arthritis (RA). Since phosphoinositide 3-kinase (PI3-K) plays a critical role in osteoclastogenesis and bone resorption, we examined the effects of ZSTK474, a novel phosphoinositide 3-kinase (PI3-K)-specific inhibitor, on murine OCs in vitro and in vivo.
The inhibitory effect of ZSTK474 on OC formation was determined and compared with other PI3-K inhibitors by counting tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells after culturing murine bone marrow monocytic OC precursors, and RAW264.7 cells. Activation of Akt and expression of nuclear factor of activated T cells (NFAT) c1 in cultured RAW264.7 cells were examined. The suppressing effect of ZSTK474 on bone resorption was assessed by the pit formation assay. The in vivo effects of ZSTK474 were studied in collagen-induced arthritis (CIA) in the mouse. Oral daily administration of ZSTK474 was started either when more than half or when all mice developed arthritis. Effects of ZSTK474 were evaluated using the arthritis score and histological score of the hind paws.
ZSTK474 inhibited the differentiation of bone marrow OC precursors and RAW264.7 cells in a dose-dependent manner. The inhibitory effect of ZSTK474 was much stronger than that of LY294002, the most commonly used PI3-K inhibitor. In addition, ZSTK474 suppressed the bone resorbing activity of mature OCs. Moreover, oral daily administration of ZSTK474, even when begun after the development of arthritis, ameliorated CIA in mice without apparent toxicity. Histological examination of the hind paw demonstrated noticeable reduction of inflammation and of cartilage destruction in ZSTK474-treated mice. ZSTK474 also significantly decreased OC formation adjacent to the tarsal bone of the hind paw.
These findings suggest that inhibition of PI3-K with ZSTK474 may potentially suppress synovial inflammation and bone destruction in patients with RA.
Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic inflammation of the synovium as well as by destruction of inflamed joints through bone erosion. The management of patients with RA consists of both reduction of inflammation and protection of the joints from structural damage . Some anti-rheumatic drugs, including biologics, are quite useful but are not effective in all patients; hence, new therapeutic agents are required.
It has been speculated that joint destruction is directly caused by osteoclasts (OCs) , which differentiate from monocytic precursors that have infiltrated the inflamed joints. After this infiltration, monocytic precursors convert to tartrate -resistant acid phosphatase (TRAP)-positive cells and fuse with each other, eventually forming giant multinucleated OCs. Although the growth and differentiation of OCs mainly depend on receptor activator of nuclear factor κB ligand (RANKL) and macrophage-colony stimulating factor (M-CSF), proinflammatory cytokines, such as tumor necrosis factor (TNF)-α, which are over-expressed in the inflamed joints, promote this process . After differentiation, ανβ3 integrins on differentiated OCs engage with the bone extracellular matrix; this process is followed by bone resorption [4, 5]. It has been demonstrated that this increased resorbing activity of OCs results not only in bone erosion and further joint destruction but also in systemic osteoporosis in patients with RA. Therefore, suppressing OCs is a major aspect of RA therapy [6, 7].
Signal transduction via the phosphoinositide 3-kinase (PI3-K)/Akt pathway is essential for regulating cellular responses, such as proliferation, survival, migration, motility and tumorigenesis, in a variety of cell types , not just OCs. Class I PI3-Ks are heterodimers and are found in four isoforms. Class IA PI3-Ks (PI3-Kα, PI3-Kβ and PI3-Kδ) are composed of a catalytic subunit p110 (α, β, or δ) and a regulatory subunit p85 (α or β), and activated through tyrosine kinase signaling. The class IB PI3-K (PI3-Kγ) is a heterodimer consisting of a catalytic subunit p110γ associated with one of two regulatory subunits, p101 and p84, and activated via seven-transmembrane G-protein-coupled receptors (GPCRs) . Whereas the expression of PI3-Kα and PI3-Kβ is ubiquitous, that of PI3-Kδ and PI3-Kγ is mainly restricted to hematopoietic cells .
Many signal transduction molecules are involved in different phases of growth and development in OCs, such as Src homology-2 (SH2)-containing inositol-5-phosphatase (SHIP), Vav3, Gab2, extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) [10–14]. In OCs, PI3-K is a major downstream effecter of the M-CSF receptor, RANK, and αβν3 integrin. The importance of PI3-K for differentiation, survival and motility of OCs has been demonstrated by using the PI3-K inhibitors wortmannin and LY294002 [15–22], and also by studying mice deficient in the expression of the p85α subunit of class IA PI3-K . In addition, several transcription factors, including NF-kB, c-fos, AP-1, PU.1, and CREB, are involved in regulating osteoclastogenesis in its early or late phase, and expression of NFATc1 is specific to the RANKL induced-signaling pathway and essential for terminal differentiation of OCs [24, 25].
Wortmannin and LY294002, potent inhibitors of PI3-K that have been extensively used for studying ex vivo PI3-K-driven signal pathways, also inhibit other related enzymes [9, 26]. LY294002 causes severe dermal toxicity , and wortmannin and its analog has shown hepatic toxicity  when administered in mice. ZSTK474, a synthesized s-triazine derivative that strongly inhibited the growth of tumor cells, was subsequently identified as a novel PI3-K-specific inhibitor [29–33]. Furthermore, ZSTK474 is suitable for oral administration, and demonstrated marked in vivo antitumor activity in mice grafted with human cancer cells without showing toxicity to major organs .
Since the action of ZSTK474 on OCs is unknown, we examined the effects of ZSTK474 in an in vitro OC culture system and found strong inhibitory effects on the differentiation and bone resorbing activity of OCs. Moreover, daily administration of ZSTK474 ameliorated collagen-induced arthritis (CIA) in mice, remarkably reducing the migration of inflammatory cells and OCs in the synovial tissue.
Materials and methods
ZSTK474 and IC87114 (a PI3-Kδ-selective inhibitor) were synthesized at Central Research Laboratories of Zenyaku Kogyo Co. Ltd. (Tokyo Japan). LY294002 was purchased from Sigma Chemical Co. (St Louis, MO, USA). AS605240 (a PI3-Kδ-selective inhibitor) was purchased from Calbiochem (Schwalbach, Germany). In in vivo experiments, ZSTK474 was prepared as a solid dispersion .
Male DBA/1 mice (eight weeks old) were purchased from Charles River Laboratories Japan (Kanagawa, Japan). They were maintained at approximately 22°C with a 12-hour light/dark cycle and given standard chow and tap water ad libitum. Newborn ddY mice were obtained from the Japan SLC, Inc. (Shizuoka, Japan). All animal experiments were approved by the local ethical committees of each institution.
In vitro OC formation was examined as previously described . Briefly, primary osteoblasts derived from growing calvarial cells of newborn ddY mice at three- to four-days of age were suspended in alpha-minimum essential medium (α-MEM, Sigma) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco BRL, Gaithersburg, MD, USA), 100 U/ml penicillin and 100 μg/ml streptomycin, and plated at a density of 2 × 104 cells/well in 24-well plates (Corning Incorporated, Corning, NY, USA) overnight. Mouse bone marrow cells containing monocytic OC precursors were removed aseptically from the tibiae of four- to six-week old ddY male mice, and co-cultured on adherent osteoblasts at a density of 1.0 × 106cells/well in medium containing 10-7 M 1α,25-(OH)2D3 (Wako Pure Chemical Industries, Ltd., Osaka, Japan) for five to six days in the presence or absence of varying concentrations of ZSTK474 or other PI3-K inhibitors. Otherwise, non-adherent bone marrow cells were cultured alone with 10 ng/ml of M-CSF (R & D Systems, Minneapolis, MN, USA) for two days, and then adherent cells were cultured with 100 ng/ml of soluble RANKL (sRANKL) (R & D Systems) for three days. In some experiments, RAW264.7 cells (American Type Culture Collection, Manassas, VA, USA) were plated at a density of 2.5 × 104 cells/well in a 24-well tissue culture plate overnight, and sRANKL (100 ng/ml), TNF-α (50 ng/ml) and ZSTK474 were added. The medium was changed every two to three days. The cells were fixed with 3.7% formalin, permeabilized with 0.1% Triton X-100, and stained with TRAP. OC formation was determined by counting TRAP-positive multinucleated cells having three or more nuclei, and OCs were counted in each set of duplicated wells.
Real time-polymerase chain reaction (PCR) for the quantification of RANKL expression
The osteoblasts were plated at a density of 2 × 105 cells/well in six-well plates, and cultured with or without 1α,25-(OH)2D3 for 24 hours in the presence or absence of ZSTK474. Total RNA was extracted using a total RNA isolation kit (Ambion Inc., Austin. TX, USA), and 3 μg of the total RNA was reverse transcribed using a You-prime Fast-Strand Breads kit (Amersham Pharmacia Biotech, Inc., Piscataway, NJ, USA). The primers used in PCR were 5'-GACTCGACTCTGGAGAGT-3' (sense primer) and 5'-GAGAACTTGGGATTTTGATGC-3' (antisense primer) for RANKL and 5'-AGCCATGTACGTAGCCATCC (sense primer) and 3'-CTCTCAGCTGTGGTGGTGAA (antisense primer) for β-actin. Real-time PCR was performed using 1 μg of cDNA and Power SYBR Green Master Mix (Applied Bio Systems, Foster City, CA, USA) on an ABI PRISM 7500 Sequence Detection System (Applied Bio Systems) with conditions at 95°C for 10 minutes, followed by 40 cycles at 95°C for 15 seconds and 60°C for one minute. The expression of RANKL was quantified using the comparative CT, applying the formula Xn = 2-ΔCT, where Xn is the relative amount of target gene in question and ΔCT is the difference between the CT of the house keeping gene for a given sample .
Western blotting for Akt and NFATc1
RAW264.7 cells were plated at a density of 2.5 × 105 cells/well in a six-well tissue culture plate overnight, and ZSTK474 was added. After incubation for 30 minutes, 50 to 100 ng/ml of sRANKL, or sRANKL plus TNF-α (50 ng/ml), was added and the cells were incubated for the indicated time. Cells were washed twice with ice-cold phosphate-buffered saline (PBS) containing 1% phosphatase inhibitor cocktail (Sigma), detached with a cell scraper, centrifuged, and lysed with lysis buffer (1% Triton X-100, 1% phosphatase inhibitor cocktail and 1 mM of PMSF in Tris-buffer, pH 7.6). The lysates were boiled with sodium dodecyl sulfate (SDS) -sample buffer and run on SDS-PAGE followed by blotting with a 1:1000 dilution of anti-phospholylated Akt (anti-phospho Akt), anti-Akt, anti-IκB, anti-phospho cJun, anti-phospho p42/p44, anti-β-actin (Cell Signal Technology, Inc., Beverly, MA, USA) and anti-NFAT1c monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
RAW264.7 cells (200 μl, 2.5 × 105/ml) were plated onto Lab Tek Chamber slide (Thermo Fisher Scientific, Rochester, NY, USA) overnight. After treatment with 0.1 μM of ZSTK474 for 30 minutes, 100 ng/ml of sRANKL and 50 mg/ml of TNF-α were added, and the cells were cultured for 48 hours. Then, the cells were fixed with 4% paraformaldehyde, washed with PBS three times, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 10% normal goat serum (Nichrei, Tokyo, Japan). The cells were incubated with anti-NFATc1 antibody diluted in PBS (1:50) for one hour, washed with PBS, and followed with phycoerythrin-conjugated goat anti-rabbit IgM+IgG (H+L chain specific, Beckman Coulter) for another one hour. The cells were postfixed in Aqua-Poly/Mount (Polysciences, Washington, PA, USA) and viewed using fluorescence microscope (Nikon ECLIPSE E600/Y-FL).
Bone resorbing activity of OC
A 10 cm culture dish (Corning) was coated with 5 ml of type I collagen mixture at 4°C. The dish was placed in a CO2 incubator at 37°C for 10 minutes to render the aqueous type I collagen gelatinous. Primary osteoblasts (5 × 105 cells/dish) and bone marrow cells (6 × 106 cells/dish) were co-cultured on the collagen gel-coated dish for five days. The dish was then treated with 4 ml of 0.2% collagenase solution (Nitta Gelatin Co., Osaka, Japan) for 20 minutes at 37°C in a shaking water bath (60 cycles/minute). The cells were collected by centrifugation at 600 rpm for three minutes, then washed and suspended with α-MEM containing 10% FBS (OC preparation). Dentine slices (Immunodiagnostic Systems, Ltd., Boldon, UK) were cleaned by ultrasonication in distilled water, sterilized using 70% ethanol, dried under ultraviolet light, and placed in 96-well plates. A 0.1-ml aliquot of the OC preparation was transferred onto the slices. After incubation for 72 hours in the presence or absence of the PI3-K inhibitors, the medium was removed and 1 ml of 1 M NH4OH was added to each well and incubated for 30 minutes. The dentin slices were then cleaned by ultrasonication, stained with hematoxylin (Wako Pure Chemical Industries) for 35 to 45 seconds, and washed with distilled water. The area of resorption pits that formed on dentine slices was observed under a light microscope and measured.
CIA in mice
Male DBA/1 mice, eight-weeks of age, were injected intradermally in the base of the tail with 200 μg of bovine type II collagen (Collagen Gijutsu Kenshu-Kai., Tokyo, Japan) emulsified in complete Freund's adjuvant (Difco, Detroit, MI, USA) on Day 1, and the same amount of the antigen emulsified in incomplete Freund's adjuvant (Difco) on Day 22. When half of the mice had developed arthritis (Day 28), the mice were randomly divided into four groups of eight mice. Each group orally received vehicle or 25, 50, 100 mg/kg of ZSTK474, once/day. In another therapeutic protocol, 100 mg/kg of ZSTK474 was administered from the day when all mice developed arthritis (Day 31). Total arthritis score was defined as the sum of the paw swelling scores for each paw (0 to 4 per paw), with a maximum score of 16. In the semi-therapeutic protocol, the mice were killed on Day 50, and the right hind paws were removed, fixed in paraformaldehyde, decalcified in Kalkitox (Wako Pure Chemical Industries, Ltd.), embedded in paraffin and sectioned. The sections were then stained with hematoxylin and eosin (H&E) or safranin O to assess hyperplasia of synovial tissue, infiltration of leukocytes, and destruction of cartilage. Each parameter was graded separately and assigned a severity score as follows: grade 0, no detectable change: 1 to 4, slight to severe changes. The number of OC in talus was counted in every third 6 μm section. To examine in vivo OC formation in CIA mice, the hind paws were removed on Day 52 and rapidly frozen in the therapeutic protocol. The frozen tissue was sectioned according to the method described previously  and the sections were stained with H&E or TRAP. Plasma TRACP5b levels were measured using a mouse TRAP™ Assay (Immunodiagnostic System Ltd).
Statistical significance of differences was assessed by one-way analysis of variance (ANOVA) followed by Dunnett's test or the Student's t-test for comparison of two samples. Statistical tests were performed using Kaleida graph 3.6 (Synergy Software, Reading, PA, USA). In all analyses, P < 0.05 was considered statistically significant.
Inhibitory effects of ZSTK474 on OC formation in co-culture system
Inhibition of Akt phosphorylation and NFATc1 expression in RAW264.7 cells by ZSTK474
Inhibitory effects of ZSTK474 on OC formation induced by both RANKL and TNF-α
We next examined the effects of ZSTK474 on OC formation induced by RANKL and TNF-α, since it was speculated that TNF-α enhanced OC formation in RA. In fact, RANKL-induced phosphorylation of Akt was enhanced by the addition of TNF-α (Figure 2d). ZSTK474 (0.03, 0.1, and 0.3 μM) inhibited the phosphorylation of Akt induced by RANKL (100 ng/ml) and TNF-α (50 ng/ml) in RAW264.7 cells (Figure 2d). Moreover, the OC formation induced by RANKL (100 ng/ml) and TNF-α (50 ng/ml) was inhibited by ZSTK474 in a dose-dependent manner. OC formation was completely inhibited by ZSTK474 (0.3 μM, Figure 2e).
Inhibition of bone resorbing activity of OC by ZSTK474
Amelioration of CIA in mice with oral administration of ZSTK474
Histological score and osteoclast number
Vehicle (n = 6)
2.3 ± 0.8
1.7 ± 0.9
2.7 ± 0.6
62.0 ± 38.6
(n = 6, 50 mg/kg)
0.0 ± 0.0
0.0 ± 0.0
0.8 ± 0.3
0.3 ± 0.2
In this study, we demonstrated that ZSTK474, a novel PI3-K-specific inhibitor, suppressed osteoclastogenesis and bone resorption. The in vitro inhibitory effect of ZSTK474 on OC formation, observed by culturing bone marrow cells, was much stronger than that of LY294002. Although both inhibit all isoforms of class I PI3-K, the inhibitory activities of ZSTK474 (IC50: PI3-Kα: 1.6 × 10-8 M; PI3-Kβ: 4.4 × 10-8 M; PI3-Kγ: 4.9 × 10-8 M; PI3-Kδ: 4.6 × 10-9 M) were much stronger than those of LY294002 (IC50: PI3-Kα: 5.5 × 10-7 M; PI3-Kβ: 1.1 × 10-5 M; PI3-Kγ: 1.2 × 10-5 M; PI3-Kδ: 1.6 × 10-6 M) on all isoforms, especially PI3-Kδ . A PI3-Kδ-selective inhibitor, IC87114 (1 μM), completely inhibited OC formation, while a PI3-Kγ-selective inhibitor, AS605240, had no inhibitory effect on OC formation. These results indicate the involvement of PI3-Kδ in the OC culture system, consistent with a previous report which implicated a critical role of class IA PI3-K in OC formation by demonstrating that OC progenitor cells from mice lacking p85α, a regulatory subunit of class IA PI3-K, showed impaired growth and differentiation .
Blocking of the phosphorylation of Akt by ZSTK474 in RAW264.7 cells indicated that the inhibitory effect on OC formation observed in the bone marrow monocytic cells was due at least in part to suppression of PI3-K/Akt signal pathway in the OC precursors. This suggestion is supported by the observation that the consequent expression of NFATc1, an essential factor for terminal RANKL-induced differentiation of OCs [25, 38], was also prevented by ZSTK474. The reduced expression of NFATc1 was dependent on neither NFkB nor cFos in the condition of this study. Additionally, translocation of NFATc1 into the nucleus was also inhibited by ZSTK474, implying that ZSTK474 might suppress the autoamplification, calcium-signal-mediated persistent activation , of NFATc1. Moreover, ZSTK474 inhibited the phosphorylation of Akt and OC differentiation induced by both RANKL and TNF-α, which are fundamental factors for OC formation in RA, implying that ZSTK474 might inhibit OC formation in patients with RA.
ZSTK474 also suppressed the bone resorbing activity of OCs as assessed in an in vitro pit formation assay. This could be explained by the inhibitory effect of ZSTK474 on survival of mature OCs in part. Likewise, signaling via PI3-K is crucial for remodeling and assembly of actin filaments, cell spreading and adhesion . Furthermore, blocking PI3-K with ZSTK474 inhibited the membrane ruffling induced by platelet-derived growth factor (PDGF) in murine embryonic fibroblasts . In OCs, the SH3 domain of tyrosine kinase, c-src, interacts with the p85 regulatory domain of PI3-K, and this signaling pathway is crucial for colony-stimulating factor-1-induced OC spreading . Therefore, ZSTK474 might suppress the cytoskeletal change of OCs, resulting in the reduced bone resorption observed in this study.
ZSTK474 suppressed inflammation and also protected against joint destruction in CIA in mice. Although it is difficult to ascertain the direct effect of ZSTK474 on OCs in this model, the TRAP-staining of the synovial tissue sections demonstrated marked reduction of OC formation. In addition, plasma levels of TRACP5b, that reported to correspond with systemic but not localized bone resorption , were not increased in 100 mg/kg ZSTK474-treated mice. This result implied that 100 mg/kg of ZSTK474 possibly prevented the systemic bone resorption.
Both the semi-therapeutic and therapeutic treatments of ZSTK474 ameliorated joint inflammation in a mouse model of RA. This anti-rheumatic effect might be explained by contribution of PI3-K to activation, proliferation and migration of inflammatory cells, such as lymphocytes, macrophages, neutrophils, mast cells and synovial fibroblasts . However, the titers of antibody to type II collagen were not significantly different between vehicle- and ZSTK474-treated mice in this experiment (data not shown). Regarding migration, chemokine receptors, such as the MCP-1 receptor and the RANTES receptor, are GPCRs that associate with PI3-Kγ and induce signals for chemotaxis of the inflammatory cells . It was reported that the PI3-Kγ-selective inhibitor suppressed joint inflammation in mouse CIA by inhibiting migration of neutrophils to the joints . This inhibitory process might occur in the ZSTK474-treated mice. Additionally, synovial pannus tissues of patients with RA express phosphorylated Akt  and exhibit tumor-like behaviors, such as angiogenesis, proliferation and invasion. A recent report demonstrated potent antiangiogenic activity for ZSTK474, which could be attributed to both inhibition of VEGF secretion by cancer cells and inhibition of PI3-K in endothelial cells . These findings also account for the effects of ZSTK474 on CIA mice. In addition, ZSTK474 did not affect the count of peripheral white blood cells and red blood cells (data not shown). Further studies are underway to evaluate how ZSTK474 exerts anti-inflammatory activity in vivo.
Clinical studies have demonstrated that the degree of inflammation and the progression of joint destruction do not always correspond with each other [46, 47]. In current therapy for RA, anti-rheumatic drugs are required not only to control the inflammation but also to suppress the joint destruction. On the other hand, recent reports have shown convincing pathogenic evidence for the involvement of class I PI3-K and Akt signaling pathways in synovial fibroblasts [44, 48–52] and other cells [43, 53, 54] in patients with RA. Synovial tissue from patients with RA expressed higher levels of phosphorylated Akt than that from patients with osteoarthritis . Moreover, blocking the PI3-K/Akt pathway by intracellular gene transfer of phosphatate and tensin homolog deleted on chromosome 10 (PTEN), which dephosphorylates phosphatidylinositol - 3,4,5 - tris - phosphate (Ptdlns(3,4,5)P3) and attenuates the downstream signals of PI3-K, CIA in rats . Taken together, the present results indicate that PI3-K could be a potent target for RA therapy.
We have demonstrated inhibitory effects of ZSTK474 on in vitro OC formations and CIA in mice. Inhibition of PI3-K with ZSTK474 may potentially have an anti-rheumatic effect in patients with RA.
extracellular signal-regulated kinase
fetal bovine serum
mitogen-activated protein kinase
macrophage-colony stimulating factor
nuclear factor of activated T cells c1
platelet-derived growth factor
phosphatate and tensin homolog deleted chromosome 10
receptor activator of nuclear factor κB
Src homology-2 (SH2)-containing inositol-5-phosphatase
tumor necrosis factor
tartrate-resistant acid phosphatase
alpha-minimum essential medium
We thank Asako Sasaki (Central Research Laboratory, Zenyaku Kogyo Co., Ltd, Tokyo, Japan) and Naoki Ishihara (Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan) for technical support. We also owe thanks to Shin-ichi Yaguchi (Zenyaku Kogyo Co., Ltd) who gave insightful comments and Makoto Fukae and Shinichiro Oida (Department of Biochemistry, School of Dental Medicine, Tsurumi University, Yokohama, Japan) who supported the set up of the in vitro experiments.
- Combe B, Landewe R, Lukas C, Bolosiu HD, Breedveld F, Dougados M, Emery P, Ferraccioli G, Hazes JM, Klareskog L, Machold K, Martin-Mola E, Nielsen H, Silman A, Smolen J, Yazici H: EULAR recommendations for the management of early arthritis: report of a task force of the European Standing Committee for International Clinical Studies Including Therapeutics (ESCISIT). Ann Rheum Dis. 2007, 66: 34-45. 10.1136/ard.2005.044354.PubMed CentralView ArticlePubMedGoogle Scholar
- Redlich K, Hayer S, Ricci R, David J, Tohidast-Akrad M, Kollias G, Steiner G, Smolen J, Wagner E, Schett G: Osteoclasts are essential for TNF-alpha-mediated joint destruction. J Clin Invest. 2002, 110: 1419-1427.PubMed CentralView ArticlePubMedGoogle Scholar
- Teitelbaum S: Osteoclasts; culprits in inflammatory osteolysis. Arthritis Res Ther. 2006, 8: 201-10.1186/ar1857.PubMed CentralView ArticlePubMedGoogle Scholar
- Gravallese E, Manning C, Tsay A, Naito A, Pan C, Amento E, Goldring S: Synovial tissue in rheumatoid arthritis is a source of osteoclast differentiation factor. Arthritis Rheum. 2000, 43: 250-258. 10.1002/1529-0131(200002)43:2<250::AID-ANR3>3.0.CO;2-P.View ArticlePubMedGoogle Scholar
- Shigeyama Y, Pap T, Kunzler P, Simmen B, Gay R, Gay S: Expression of osteoclast differentiation factor in rheumatoid arthritis. Arthritis Rheum. 2000, 43: 2523-2530. 10.1002/1529-0131(200011)43:11<2523::AID-ANR20>3.0.CO;2-Z.View ArticlePubMedGoogle Scholar
- Schett G: Osteoimmunology in rheumatic diseases. Arthritis Res Ther. 2009, 11: 210-10.1186/ar2571.PubMed CentralView ArticlePubMedGoogle Scholar
- Schett G, Stach C, Zwerina J, Voll R, Manger B: How antirheumatic drugs protect joints from damage in rheumatoid arthritis. Arthritis Rheum. 2008, 58: 2936-2948. 10.1002/art.23951.View ArticlePubMedGoogle Scholar
- Rückle T, Schwarz M, Rommel C: PI3Kgamma inhibition: towards an 'aspirin of the 21st century'?. Nat Rev Drug Discov. 2006, 5: 903-918. 10.1038/nrd2145.View ArticlePubMedGoogle Scholar
- Rommel C, Camps M, Ji H: PI3K delta and PI3K gamma: partners in crime in inflammation in rheumatoid arthritis and beyond?. Nat Rev Immunol. 2007, 7: 191-201. 10.1038/nri2036.View ArticlePubMedGoogle Scholar
- Faccio R, Teitelbaum S, Fujikawa K, Chappel J, Zallone A, Tybulewicz V, Ross F, Swat W: Vav3 regulates osteoclast function and bone mass. Nat Med. 2005, 11: 284-290. 10.1038/nm1194.View ArticlePubMedGoogle Scholar
- Faccio R, Zou W, Colaianni G, Teitelbaum S, Ross F: High dose M-CSF partially rescues the Dap12-/- osteoclast phenotype. J Cell Biochem. 2003, 90: 871-883. 10.1002/jcb.10694.View ArticlePubMedGoogle Scholar
- Wada T, Nakashima T, Oliveira-dos-Santos A, Gasser J, Hara H, Schett G, Penninger J: The molecular scaffold Gab2 is a crucial component of RANK signaling and osteoclastogenesis. Nat Med. 2005, 11: 394-399. 10.1038/nm1203.View ArticlePubMedGoogle Scholar
- Takeshita S, Namba N, Zhao J, Jiang Y, Genant H, Silva M, Brodt M, Helgason C, Kalesnikoff J, Rauh M, Humphries R, Krystal G, Teitelbaum S, Ross F: SHIP-deficient mice are severely osteoporotic due to increased numbers of hyper-resorptive osteoclasts. Nat Med. 2002, 8: 943-949. 10.1038/nm752.View ArticlePubMedGoogle Scholar
- Lee S, Woo K, Kim S, Kim H, Kwack K, Lee Z, Kim H: The phosphatidylinositol 3-kinase, p38, and extracellular signal-regulated kinase pathways are involved in osteoclast differentiation. Bone. 2002, 30: 71-77. 10.1016/S8756-3282(01)00657-3.View ArticlePubMedGoogle Scholar
- Nakamura I, Takahashi N, Sasaki T, Tanaka S, Udagawa N, Murakami H, Kimura K, Kabuyama Y, Kurokawa T, Suda T: Wortmannin, a specific inhibitor of phosphatidylinositol-3 kinase, blocks osteoclastic bone resorption. FEBS Lett. 1995, 361: 79-84. 10.1016/0014-5793(95)00153-Z.View ArticlePubMedGoogle Scholar
- Nakagawa N, Kinosaki M, Yamaguchi K, Shima N, Yasuda H, Yano K, Morinaga T, Higashio K: RANK is the essential signaling receptor for osteoclast differentiation factor in osteoclastogenesis. Biochem Biophys Res Commun. 1998, 253: 395-400. 10.1006/bbrc.1998.9788.View ArticlePubMedGoogle Scholar
- Pilkington M, Sims S, Dixon S: Wortmannin inhibits spreading and chemotaxis of rat osteoclasts in vitro. J Bone Miner Res. 1998, 13: 688-694. 10.1359/jbmr.19220.127.116.118.View ArticlePubMedGoogle Scholar
- Palacio S, Felix R: The role of phosphoinositide 3-kinase in spreading osteoclasts induced by colony-stimulating factor-1. Eur J Endocrinol. 2001, 144: 431-440. 10.1530/eje.0.1440431.View ArticlePubMedGoogle Scholar
- Lee S, Chung W, Kwak H, Chung C, Kwack K, Lee Z, Kim H: Tumor necrosis factor-alpha supports the survival of osteoclasts through the activation of Akt and ERK. J Biol Chem. 2001, 276: 49343-49349. 10.1074/jbc.M103642200.View ArticlePubMedGoogle Scholar
- Gingery A, Bradley E, Shaw A, Oursler M: Phosphatidylinositol 3-kinase coordinately activates the MEK/ERK and AKT/NFkappaB pathways to maintain osteoclast survival. J Cell Biochem. 2003, 89: 165-179. 10.1002/jcb.10503.View ArticlePubMedGoogle Scholar
- Bradley E, Ruan M, Vrable A, Oursler M: Pathway crosstalk between Ras/Raf and PI3K in promotion of M-CSF-induced MEK/ERK-mediated osteoclast survival. J Cell Biochem. 2008, 104: 1439-1451. 10.1002/jcb.21719.PubMed CentralView ArticlePubMedGoogle Scholar
- Grey A, Chen Y, Paliwal I, Carlberg K, Insogna K: Evidence for a functional association between phosphatidylinositol 3-kinase and c-src in the spreading response of osteoclasts to colony-stimulating factor-1. Endocrinology. 2000, 141: 2129-2138. 10.1210/en.141.6.2129.PubMedGoogle Scholar
- Munugalavadla V, Vemula S, Sims E, Krishnan S, Chen S, Yan J, Li H, Niziolek P, Takemoto C, Robling A, Yang F, Kapur R: The p85alpha subunit of class IA phosphatidylinositol 3-kinase regulates the expression of multiple genes involved in osteoclast maturation and migration. Mol Cell Biol. 2008, 28: 7182-7198. 10.1128/MCB.00920-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Asagiri M, Takayanagi H: The molecular understanding of osteoclast differentiation. Bone. 2007, 40: 251-264. 10.1016/j.bone.2006.09.023.View ArticlePubMedGoogle Scholar
- Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, Saiura A, Isobe M, Yokochi T, Inoue J, Wagner E, Mak T, Kodama T, Taniguchi T: Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell. 2002, 3: 889-901. 10.1016/S1534-5807(02)00369-6.View ArticlePubMedGoogle Scholar
- Kong D, Dan S, Yamazaki K, Yamori T: Inhibition profiles of phosphatidylinositol 3-kinase inhibitors against PI3K superfamily and human cancer cell line panel JFCR39. Eur J Cancer. 2010, 46: 1111-1121. 10.1016/j.ejca.2010.01.005.View ArticlePubMedGoogle Scholar
- Hu L, Zaloudek C, Mills G, Gray J, Jaffe R: In vivo and in vitro ovarian carcinoma growth inhibition by a phosphatidylinositol 3-kinase inhibitor (LY294002). Clin Cancer Res. 2000, 6: 880-886.PubMedGoogle Scholar
- Ihle N, Williams R, Chow S, Chew W, Berggren M, Paine-Murrieta G, Minion D, Halter R, Wipf P, Abraham R, Kirkpatrick L, Powis G: Molecular pharmacology and antitumor activity of PX-866, a novel inhibitor of phosphoinositide-3-kinase signaling. Mol Cancer Ther. 2004, 3: 763-772.PubMedGoogle Scholar
- Yaguchi S, Fukui Y, Koshimizu I, Yoshimi H, Matsuno T, Gouda H, Hirono S, Yamazaki K, Yamori T: Antitumor activity of ZSTK474, a new phosphatidylinositol 3-kinase inhibitor. J Natl Cancer Inst. 2006, 98: 545-556. 10.1093/jnci/djj133.View ArticlePubMedGoogle Scholar
- Kong D, Yamori T: ZSTK474 is an ATP-competitive inhibitor of class I phosphatidylinositol 3 kinase isoforms. Cancer Sci. 2007, 98: 1638-1642. 10.1111/j.1349-7006.2007.00580.x.View ArticlePubMedGoogle Scholar
- Kong D, Yamori T: Phosphatidylinositol 3-kinase inhibitors: promising drug candidates for cancer therapy. Cancer Sci. 2008, 99: 1734-1740. 10.1111/j.1349-7006.2007.00693.x.View ArticlePubMedGoogle Scholar
- Kong D, Yaguchi S, Yamori T: Effect of ZSTK474, a novel phosphatidylinositol 3-kinase inhibitor, on DNA-dependent protein kinase. Biol Pharm Bull. 2009, 32: 297-300. 10.1248/bpb.32.297.View ArticlePubMedGoogle Scholar
- Kong D, Yamori T: Advances in development of phosphatidylinositol 3-kinase inhibitors. Curr Med Chem. 2009, 16: 2839-2854. 10.2174/092986709788803222.View ArticlePubMedGoogle Scholar
- Sugama T, Ishihara N, Tanaka Y, Takahashi M, Yaguchi S, Watanabe T: Amorphous form of heterocyclic compound, solid dispersion and medicinal preparation each comprising the same, and process for production of the same. Patents. WO2009066775. 2009Google Scholar
- Suda T, Jimi E, Nakamura I, Takahashi N: Role of 1 alpha,25-dihydroxyvitamin D3 in osteoclast differentiation and function. Methods Enzymol. 1997, 282: 223-235. full_text.View ArticlePubMedGoogle Scholar
- Tsubaki M, Kato C, Manno M, Ogaki M, Satou T, Itoh T, Kusunoki T, Tanimori Y, Fujiwara K, Matsuoka H, Nishida S: Macrophage inflammatory protein-1alpha (MIP-1alpha) enhances a receptor activator of nuclear factor kappaB ligand (RANKL) expression in mouse bone marrow stromal cells and osteoblasts through MAPK and PI3K/Akt pathways. Mol Cell Biochem. 2007, 304: 53-60. 10.1007/s11010-007-9485-7.View ArticlePubMedGoogle Scholar
- Kawamoto T: Use of a new adhesive film for the preparation of multi-purpose fresh-frozen sections from hard tissues, whole-animals, insects and plants. Arch Histol Cytol. 2003, 66: 123-143. 10.1679/aohc.66.123.View ArticlePubMedGoogle Scholar
- Ikeda F, Nishimura R, Matsubara T, Tanaka S, Inoue J, Reddy S, Hata K, Yamashita K, Hiraga T, Watanabe T, Kukita T, Yoshioka K, Rao A, Yoneda T: Critical roles of c-Jun signaling in regulation of NFAT family and RANKL-regulated osteoclast differentiation. J Clin Invest. 2004, 114: 475-484.PubMed CentralView ArticlePubMedGoogle Scholar
- Matsuo K, Galson D, Zhao C, Peng L, Laplace C, Wang K, Bachler M, Amano H, Aburatani H, Ishikawa H, Wagner E: Nuclear factor of activated T-cells (NFAT) rescues osteoclastogenesis in precursors lacking c-Fos. J Biol Chem. 2004, 279: 26475-26480. 10.1074/jbc.M313973200.View ArticlePubMedGoogle Scholar
- Takayanagi H: Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. Nat Rev Immunol. 2007, 7: 292-304. 10.1038/nri2062.View ArticlePubMedGoogle Scholar
- Rivard N: Phosphatidylinositol 3-kinase: a key regulator in adherens junction formation and function. Front Biosci. 2009, 14: 510-522. 10.2741/3259.View ArticleGoogle Scholar
- Stolina M, Adamu S, Ominsky M, Dwyer D, Asuncion F, Geng Z, Middleton S, Brown H, Pretorius J, Schett G, Bolon B, Feige U, Zack D, Kostenuik P: RANKL is a marker and mediator of local and systemic bone loss in two rat models of inflammatory arthritis. J Bone Miner Res. 2005, 20: 1756-1765. 10.1359/JBMR.050601.View ArticlePubMedGoogle Scholar
- Camps M, Rückle T, Ji H, Ardissone V, Rintelen F, Shaw J, Ferrandi C, Chabert C, Gillieron C, Françon B, Martin T, Gretener D, Perrin D, Leroy D, Vitte P, Hirsch E, Wymann M, Cirillo R, Schwarz M, Rommel C: Blockade of PI3Kgamma suppresses joint inflammation and damage in mouse models of rheumatoid arthritis. Nat Med. 2005, 11: 936-943.PubMedGoogle Scholar
- Zhang H, Wang Y, Xie J, Liang X, Liu D, Yang P, Hsu H, Ray R, Mountz J: Regulation of tumor necrosis factor alpha-mediated apoptosis of rheumatoid arthritis synovial fibroblasts by the protein kinase Akt. Arthritis Rheum. 2001, 44: 1555-1567. 10.1002/1529-0131(200107)44:7<1555::AID-ART279>3.0.CO;2-M.View ArticlePubMedGoogle Scholar
- Kong D, Okamura M, Yoshimi H, Yamori T: Antiangiogenic effect of ZSTK474, a novel phosphatidylinositol 3-kinase inhibitor. Eur J Cancer. 2009, 45: 857-865. 10.1016/j.ejca.2008.12.007.View ArticlePubMedGoogle Scholar
- Smolen J, van der Heijde D, St Clair E, Emery P, Bathon J, Keystone E, Maini R, Kalden J, Schiff M, Baker D, Han C, Han J, Bala M: Predictors of joint damage in patients with early rheumatoid arthritis treated with high-dose methotrexate with or without concomitant infliximab: results from the ASPIRE trial. Arthritis Rheum. 2006, 54: 702-710. 10.1002/art.21678.View ArticlePubMedGoogle Scholar
- Smolen J, Han C, van der Heijde D, Emery P, Bathon J, Keystone E, Maini R, Kalden J, Aletaha D, Baker D, Han J, Bala M, St Clair E: Radiographic changes in rheumatoid arthritis patients attaining different disease activity states with methotrexate monotherapy and infliximab plus methotrexate: the impacts of remission and tumour necrosis factor blockade. Ann Rheum Dis. 2009, 68: 823-827. 10.1136/ard.2008.090019.View ArticlePubMedGoogle Scholar
- Kim G, Jun J, Elkon K: Necessary role of phosphatidylinositol 3-kinase in transforming growth factor beta-mediated activation of Akt in normal and rheumatoid arthritis synovial fibroblasts. Arthritis Rheum. 2002, 46: 1504-1511. 10.1002/art.10314.View ArticlePubMedGoogle Scholar
- Chen Q, Casali B, Pattacini L, Boiardi L, Salvarani C: Tumor necrosis factor-alpha protects synovial cells from nitric oxide induced apoptosis through phosphoinositide 3-kinase Akt signal transduction. J Rheumatol. 2006, 33: 1061-1068.PubMedGoogle Scholar
- Morel J, Audo R, Hahne M, Combe B: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces rheumatoid arthritis synovial fibroblast proliferation through mitogen-activated protein kinases and phosphatidylinositol 3-kinase/Akt. J Biol Chem. 2005, 280: 15709-15718. 10.1074/jbc.M414469200.View ArticlePubMedGoogle Scholar
- Traister R, Fabre S, Wang Z, Xiao X, Hirsch R: Inflammatory cytokine regulation of transgene expression in human fibroblast-like synoviocytes infected with adeno-associated virus. Arthritis Rheum. 2006, 54: 2119-2126. 10.1002/art.21940.View ArticlePubMedGoogle Scholar
- Wang C, Shiau A, Chen S, Lin L, Tai M, Shieh G, Lin P, Yo Y, Lee C, Kuo S, Liu M, Jou I, Yang C, Shen P, Lee H, Wu C: Amelioration of collagen-induced arthritis in rats by adenovirus-mediated PTEN gene transfer. Arthritis Rheum. 2008, 58: 1650-1656. 10.1002/art.23517.View ArticlePubMedGoogle Scholar
- Liu H, Huang Q, Shi B, Eksarko P, Temkin V, Pope R: Regulation of Mcl-1 expression in rheumatoid arthritis synovial macrophages. Arthritis Rheum. 2006, 54: 3174-3181. 10.1002/art.22132.View ArticlePubMedGoogle Scholar
- Kim K, Cho M, Park M, Yoon C, Park S, Lee S, Kim H: Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor kappaB-dependent pathway in patients with rheumatoid arthritis. Arthritis Res Ther. 2005, 7: R139-148. 10.1186/ar1470.PubMed CentralView ArticlePubMedGoogle Scholar
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