Biologic Aspects of Nurse-Like Cells Found in Bone Marrow and Synovial Tissue of Rheumatoid Arthritis Patients

  • Takahiro Ochi1

    Affiliated with

    Arthritis Res19991(Suppl 1):S20

    DOI: 10.1186/ar34

    Published: 15 November 1999

    Full text

    Using collagen-induced arthritis (CIA) rats, we studied the origin of synovial stromal cells, which rapidly appeared and proliferated in joints at the onset of inflammatory polyarthritis, we partially labelled the bone marrow stromal cells (BMSC) with fluorescent dye or 3H-Tdr,and analyzed the migration of labelled BMSC after the immunization with collagen. At the onset of CIA, BMSC migrated through small canals from the bone marrow into the affected joint cavities and seemed to contribute to synovial proliferation in joints [4].

    Based on the data above, we were interested in establishing and characterizing the nurse cell-like stromal cells (NCs) in bone marrow (RA-BMNC) as well as those in synovial tissue of rheumatoid arthritis (RA) patients (RA-SNC). RA-BMNC showed the characteristic cell-cell contact with lymphocytes (pseudoempeliporesis), resulting in mutual biologic activation, such as maintaining infiltrating lymphocytes and producing large amount of cytokines. Those were very similar to the reactions of RA-SNC reported in our latest paper [2].

    Another point of interest was whether NCs could invade the bone, resulting in erosive changes characteristically observed in RA patients. Although NCs (both RA-SNC and RA-BMNC) were shown to produce IL-6, IL-8, and other cytokines, these were not thought to contribute directly to bone erosion. By co-culturing NCs with lymphocytes, we found activation in the production of MMP-1 and MMP-3, and in the expression of mRNA of both MMP-9 and cathepsin-K. Thus, NCs could be thought to contribute directly to bone erosion in RA patients.

    Authors’ Affiliations

    (1)
    Osaka University Medical School

    References

    1. Tomita T, Ochi T, et al.: Establishment of nurse-like stromal cells from bone marrow of patients with rheumatoid arthritis: indication of characteristic bone marrow microenvironment in patients with rheumatoid arthritis. Rheumatology 1999.
    2. Takeuchi E, Ochi T, et al.: Establishment and characterization of nurse cell-like stromal cell lines from synovial tissues of patients with rheumatoid arthritis. Arthritis Rheum 1999.
    3. Wakisaka S, Ochi T, et al.: Possible correction of abnormal rheumatoid arthritis synovial cell function by jun D transfection in vivo . Arthritis Rheum 1998, 41:470–481.View ArticlePubMed
    4. Nakagawa S, Ochi T, et al.: Bone marrow stromal cells contribute to synovial cell proliferation in rats with collagen Induced arthritis. J Rheumatol 1996, 23:2098–2103.PubMed
    5. Hirohata S, Ochi T, et al.: Accelerated generation of CD14+monocyte-lineage cells from the bone marrow of rheumatoid arthritis patients. Arthritis Rheum 1996, 39:836–843.View ArticlePubMed
    6. Tomita T, Ochi T, et al.: Phenotypic characteristics of bone marrow cells in patients with rheumatoid arthritis. J Rheumatol 1994, 21:1608–1614.PubMed

    Copyright

    © Current Science Ltd 2000

    Advertisement