Impact of interleukin-21 in the pathogenesis of primary Sjogren's syndrome: increased serum levels of interleukin-21 and its expression in the labial salivary glands
- Kwi Young Kang1,
- Hyun-Ok Kim2,
- Seung-Ki Kwok3,
- Ji Hyeon Ju3,
- Kyung-Su Park3,
- Dong-Il Sun4,
- Joo Yeon Jhun5,
- Hye Jwa Oh5,
- Sung-Hwan Park†3Email author and
- Ho-Youn Kim†3Email author
© Kang et al.; licensee BioMed Central Ltd. 2011
Received: 1 May 2011
Accepted: 26 October 2011
Published: 26 October 2011
Interleukin (IL)-21 is a cytokine that controls the functional activity of effector T helper cells and the differentiation of Th17 cells, and promotes B-cell differentiation. To test whether IL-21 participates in the pathogenesis of primary Sjögren's syndrome (SS), serum IL-21 level was measured and IL-21 expression in the labial salivary glands (LSG) was examined.
Serum IL-21 levels in 40 primary SS, 40 rheumatoid arthritis (RA), and 38 systemic lupus erythematosus (SLE) patients and 20 healthy controls were measured. Serum IL-21 levels of SS patients were assessed for correlations with laboratory data, including anti-nuclear antibody, anti-Ro/La antibodies, globulin, immunoglobulin (Ig) class, and IgG subclass. LSGs from 16 primary SS and 4 controls with sicca symptoms were evaluated for IL-21 and IL-21 receptor (IL-21R) expression by immunohistochemistry. Confocal microscopy was performed to further characterize the IL-21 positive cells.
Primary SS patients had significantly higher serum IL-21 levels than controls, and these increments correlated positively with levels of IgG, IgG1. Serum IgG1 levels correlated with anti-Ro antibody titers. Immunohistochemical analyses showed that lymphocytic foci and the periductal area of the LSGs from SS patients expressed high levels of IL-21 and lower levels of IL-21R, whereas the control LSGs showed minimal expression of both antigens. The more the lymphocyte infiltrated, IL-21expression in LSGs showed a tendency to increase. Confocal microscopic analyses revealed that IL-21 expressing infiltrating lymphocytes in the LSGs of SS patients also expressed CXCR5.
Primary SS is associated with high serum IL-21 levels that correlate positively with serum IgG, especially IgG1, levels. The expression of IL-21 is increased as more lymphocytes infiltrated in LSGs. These observations suggest that IL-21 may play an important role in primary SS pathogenesis.
KeywordsIL-21 IL-21 receptor Sjogren's syndrome Immunoglobulin G1 Labial salivary gland
IL-21 is a pleiotropic cytokine that belongs to the common cytokine receptor γ chain (γc)-dependent cytokine family, which is produced by activated CD4+ T cells and NKT cells . The IL-21 receptor (IL-21R) consists of the IL-21R α chain and the γc chain and is expressed on T cells, NK cells, NKT cells, B cells, dendritic cells (DCs) and macrophages as well as on non-hematopoietic cells, including keratinocytes and fibroblasts . The activation of IL-21R by the binding of IL-21 enhances the proliferation of T cells after their prior stimulation with anti-CD3 . IL-21 also controls the functional activity of effector T helper (Th) cells and the differentiation of Th17 cells, and counteracts the suppressive effects of regulatory T cells . IL-21 alone is capable of directly inducing both B lymphocyte-induced maturation protein-1 (Blimp-1), which is required for plasma-cell differentiation, and Bcl-6, which is required for germinal center reactions . IL-21 also promotes B-cell differentiation by synergizing with BAFF and enhancing the CD40-mediated induction of activation-induced deaminase (AID) and Blimp1 . Overexpression of IL-21 in mice results in hypergammaglobulinemia and autoantibody production .
Primary Sjögren's syndrome (SS) is a systemic autoimmune disease characterized by keratoconjunctivitis sicca, xerostomia, and extraglandular abnormalities . At the immunological level, it is characterized by both T-cell lymphocytic infiltration of the exocrine glands and B-cell hyper-reactivity. Hypergammaglobulinemia is a common laboratory finding in primary SS. Since previous observations suggest that IL-21 and IL-21R may be associated with immunoglobulin production, autoantibody production, and B-lymphocyte hyperactivity [3, 9], it is thought that IL-21 is involved in the pathogenesis of autoimmune disease. However, the precise roles IL-21 and IL-21R in human autoimmune disease are still poorly understood. To determine whether IL-21 participates in primary SS pathogenesis, we examined whether the serum IL-21 levels of patients with primary SS correlate with various laboratory parameters. The expression of the IL-21/IL-21R cytokine/receptor pair by the salivary glands of patients with primary SS was also investigated.
Materials and methods
Patients and samples
The participants were selected from patients at Seoul Saint Mary's hospital at the Catholic University of Korea. Written informed consent was obtained from all patients and controls. The study was approved by the ethical committee of the Seoul St. Mary's Hospital (KC09FZZZ0522). For serological studies, serum samples were obtained from 40 patients with primary SS, 38 patients with systemic lupus erythematosus (SLE), 40 patients with rheumatoid arthritis (RA), and 20 healthy controls. All SLE and RA patients fulfilled American College of Rheumatology (ACR) classification criteria [10, 11]. The consent form was approved by the Hospital Ethics Committee. In addition, labial salivary gland (LSG) biopsy specimens were collected from 16 patients (all women) that matched the histological criteria for a diagnosis of SS  and had severe cellular infiltration (focus score ≥1). All patients were female. The mean age and the duration of disease were 51.7 ± 9.5 years and 0.3 ± 1.0 years. The biopsies were performed for routine diagnostic purposes after obtaining the patient's consent. All patients diagnosed with primary SS fulfilled the American-European Consensus Group Criteria for this diagnosis . There were also four control LSG specimens from subjects who did not fulfill the classification criteria for primary SS but had sicca symptoms, such as dry mouth or dry eyes. The controls were matched for sex and age to the primary SS patients and had been examined for the presence of rheumatic disease, including secondary SS.
Clinical and immunological data
All patients underwent extensive medical examinations and serological evaluations, including measurements of anti-nuclear antibody (ANA), rheumatoid factor (RF), globulins and Ig classes, along with the erythrocyte sedimentation rate (ESR). In addition, the anti-Ro/SSA, anti-La/SSB, and IgG subclass titers in primary SS patients were measured.
The anti-Ro/SSA and anti-La/SSB antibodies were tested by using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (BIO QUANT, San Diego, CA and USA). To measure IgG1, IgG2, IgG3 and IgG4 titers, a Human IgG Subclass Profile ELISA kit (Invitrogen, Camarillo, CA, USA) was used.
Labial salivary gland biopsy
LSG biopsies were taken from primary SS patients at an otorhinolaryngology clinic. For this, local anesthetic was injected into the lower lip and a small incision to the right or left of the lip midline was made. Five or six LSG lobules were harvested carefully and placed into 10% phosphate-buffered formalin for 24 hours. Standard paraffin preparations were prepared, sectioned at a thickness of 5 μm, and stained with hematoxylin and eosin. The slides were examined for the presence of lymphocytic infiltrates and/or foci by three observers using standardized criteria. The focus score was reported as the number of foci per 4 mm2 of tissue.
Measurement of serum IL-21
The IL-21 concentrations in the sera from the 40 primary SS patients, 38 SLE patients, 40 RA patients, and 20 healthy controls were measured by sandwich ELISA. Briefly, monoclonal capture antibodies (eBioscience, San Diego, CA and USA) were added to a 96-well plate (Nunc) and incubated overnight at 4°C, after which the plates were washed five times with PBS containing 0.05% Tween20. Following incubation with blocking solution for one hour at room temperature, the test samples and recombinant IL-21 (eBioscience) standards were added to the plates. The plates were incubated for two hours at room temperature, after which they were washed five times. Biotin-conjugated anti-human IL-21 antibodies (eBioscience) were added and, incubated for an hour at room temperature and the plates were washed. Avidin-horseradish peroxidase (eBioscience) was added and the reaction was allowed to proceed for 30 minutes at room temperature. The plates were then washed five times, and TMB solution was added to induce the color reaction, which was stopped by adding 2N H2SO4. The optical density at 450 nm was measured by using an automated microplate reader (VERSAmax, Molecular Devices, Palo Alto, CA, USA). A standard curve was drawn by plotting the optical density against the log of the concentration of IL-21.
Immunohistochemical staining for IL-21 and IL-21R
The fixed LSG biopsy specimen slides were deparaffinized by immersion in xylene, followed by dehydration in ethanol. The sections were incubated for 30 minutes at room temperature with blocking solution containing normal sera and avidin block (Vector Laboratories, Burlingame, CA, USA), and then incubated overnight at 4°C with either anti-IL-21 (Santa Cruz Technology Inc., Santa Cruz, CA, USA) or anti-IL-21R, both of which were diluted 1:100 (R&D Systems, Minneapolis, MN and USA). Mouse IgG or goat IgG served as isotype controls. The slides were washed for 5 minutes, followed by a 20-minute incubation with biotinylated anti-rabbit IgG or biotinylated anti-goat IgG (Vector). Following a 15-minute wash, the slides were incubated for 1 h with horseradish peroxidase-conjugated avidin-biotin by using the Vectastain ABC Elite (Vector). The stain was developed by using diaminobenzidine substrate (DAKO, Carpinteria, CA, USA). Counterstaining was performed with hematoxylin. Enumeration of IL-21-positive cells was performed in the vicinity of LSG lymphocytic infiltration and in the interstitium. One lymphocytic focus per specimen was randomly selected for enumerating the number of positively stained cells. Quantification of IL-21-positive cells was performed with the HistoQuest analysis software (TissueGnostics, Los Angeles, CA and USA).
Confocal microscopic analysis of LSG co-expression of CD4, CD20, CXCR5, and IL-21
Salivary gland pieces from eight patients were analyzed. Cryosections (7-μm thick) were fixed with acetone, blocked with 10% goat serum, and stained with anti-CD4-FITC, anti-CD20-FITC (BD Biosciences, Franklin Lakes, NJ and USA), anti-CXCR5-PerCP-Cy5.5 (Biolegend, San Diego, CA and USA), and anti-IL-21-PE (eBioscience). Fluorescence images were acquired by using a LSM 510 confocal microscope (Zeiss, Berlin, Brandenburg and Germany).
Statistical analyses were performed by using SAS software (Version 9) (Cary, North Carolina and USA). The data were expressed as individual values with median. Differences in the mean IL-21 levels of various groups were analyzed by using the Kruskal-Wallis test. Differences between the two groups were analyzed by using the Mann-Whitney test (two-tailed). Spearman's rank test was used to assess the correlation between the levels of IL-21 and laboratory data. Values of P < 0.05 were considered significant.
Primary SS patients have higher serum IL-21 levels than healthy controls
The serum IL-21 levels in patients with primary SS correlate with their globulin, IgG, and IgG1 levels
Multivariate regression analysis between IL-21, IgG1 and Anti-Ro Antibody
1.218 to 2.200
0.000 to 0.001
0.004 to 0.022
Expression of IL-21 and IL-21R in LSGs from patients with primary SS
Immunohistochemical analysis of IL-21 and IL-21R
The primary SS patients were divided according to their grade of lymphocytic infiltration (grades 1 to 4; described in the Methods). A grade 2 specimen is shown in Figure 4a; this specimen was obtained from a primary SS patient who fulfilled the American-European Consensus Group Criteria, except LSG biopsy. Moderate lymphocytic infiltration was observed but a definite lymphocytic focus was not seen. The specimens that had grade 3 or 4 had one or more foci. IL-21 positive cell number was increased more in both grade 3 and grade 4 than in the control, as shown in Figure 4d. Though there are no significant differences between grade 3 and grade 4, the more severe the lymphocytic infiltration was, the more IL-21 was expressed on both the infiltrating lymphocytes and periductal area (Figure 4e). However, we could not show a significant relationship between IL-21R positive cell number and the severity of lymphocytic infiltration.
Co-expression of IL-21 and CXCR5 in the LSGs of patients with primary SS
In this study, we assessed the expression pattern of IL-21 and IL-21R in LSG tissues of primary SS patients. Our results encouraged the original observation demonstrating the correlation between IL-21 expression and IgG levels in association with disease severity of SS.
The role of IL-21 in the pathogenesis of SS is poorly understood, but several lines of thought support the notion that it may contribute to a pivotal role in the process of disease. First, it is known that IL-21 is involved in the proliferation and survival of B cells, promoting their differentiation into Ig-producing plasma cells . The latter ability of IL-21 [7, 18] suggests that this cytokine may play important roles in B cell-mediated autoimmune diseases and allergies. Second, naïve IL-21R KO mice have diminished serum IgG1 levels and immunization of these mice with T-dependent antigen results in lower antigen-specific IgG1 levels compared to wild-type mice . Third, SS patients have increased IgG levels in their serum, although the reason for this increment has not been explained. The B-cell infiltration in the LSGs from primary SS patients is less than the T-cell infiltration; however, B-cell hyperactivity is an important characteristic of primary SS. Indeed, a prominent humoral autoimmune response in patients with primary SS is the production of anti-Ro/SSA antibodies. Moreover, with regard to the IgG subclasses of the anti-Ro antibodies, IgG1 predominates . In this study, we found that the serum IL-21 levels correlated with the serum globulin, IgG, and, in particular, IgG1 levels in primary SS patients. These observations are consistent with the role IL-21 plays in plasma-cell differentiation . In addition, we found the IgG1 levels correlated with the serum anti-Ro/SSA antibody titers. Thus, our observations support the notion that IL-21 may promote autoantibody production by inducing IgG class switch recombination in B cells.
IL-21 regulates the activation, proliferation and survival of CD4+ T cells as well as that of B cells [20, 21]. Specifically, it up-regulates the expression of the ROR γt transcription factor, promotes Th17-cell differentiation, and induces IL-23R expression on Th17 cells, thus facilitating the ability of IL-23 to expand the Th17-cell response [22, 23]. IL-21 also regulates the functional activity of CD8+ T cells and NK cells [20, 21]. Naive CD8+ T cells express low levels of IL-21R  and, although IL-21 does not by itself induce CD8+ T-cell proliferation, it acts synergistically with IL-15 or IL-7 to induce the proliferation of both naïve and memory phenotype CD8+ T cells .
Our study showed that IL-21 correlates with the degree of lymphocytic infiltration in the LSGs of patients with primary SS. The lymphocytic foci and periductal areas of the LSGs had many IL-21 and IL-21R positive cells. In contrast, the LSGs of healthy control subjects did not express IL-21 or IL-21R. Together these data suggest that IL-21 and IL-21Rpositive cells may play an important role in the sialoadenitis suffered by patients with primary SS.
Early studies showing that two mouse models of SLE have elevated IL-21 levels suggested that IL-21 may play a role in autoimmunity. Herber et al. showed that, in the MRL-Faslpr mouse model of SLE, blocking IL-21 with IL-21R-Fc reduced renal disease lymphadenopathy, skin lesions, and circulating autoantibodies and IgG . Similarly, administration of an IL-21R-Fc fusion protein to BXSB.B6-Yaa+/J mice, the second SLE animal model, decreased IL-21 production, lymphocyte activation and circulating IgG1 levels . These data are in agreement with the results from our study, suggesting the notion that IL-21 may participate in autoimmune disease pathogenesis by influencing lymphocytic activation and IgG production. Further supporting this possibility is that the blockade of endogenous IL-21 activity by an IL-21R-Fc fusion protein in the cultured RA synovial membrane cells significantly inhibited the production of inflammatory cytokines . Moreover, administration of IL-21R-Fc to collagen-induced arthritis mice and adjuvant-induced arthritis rats alleviated their clinical and histological signs of inflammation .
Additional lines of evidence also suggest that IL-21 may promote human autoimmune disease in general. Yuan et al. have reported that patients with SS have higher serum IL-21 levels than healthy control subjects, which correlated positively with gamma-globulin levels . Moreover, by using a large sample of SLE patients and healthy controls, Sawalha et al. found that SLE is associated with two single nucleotide polymorphisms of the IL-21 gene . In addition, patients with SLE have elevated IL-21 serum levels that correlate with the severity of the disease . Similarly, Astrid et al. have reported that synovial fibroblasts and synovial macrophages of patients with RA express IL-21R (IL-21 was not tested) . In addition, the epidermis of patients with systemic sclerosis expressed IL-21R (but not IL-21) . Recently, genome-wide association studies have provided convincing evidence that the chromosomal 4g 27 region that harbors the IL-21 and IL-21 genes is associated with chronic inflammatory disorders, including SLE, inflammatory bowel disease and psoriasis [30, 34, 35].
Interestingly, two previous studies reported that they were not able to detect IL-21 in the synovium of RA patients or the skin of systemic sclerosis patients [32, 33]. This may relate to an observation made in the experimental autoimmune encephalitis (EAE) animal model; while the treatment of mice with IL-21 after EAE was induced did not affect the severity of the disease, treatment before disease induction greatly enhanced the inflammatory influx into the central nervous system and the severity of the disease . The latter mice also exhibited higher levels of circulating myelin-specific antibodies and IFN-γ, but the level of IL-4 remained similar to that of control mice. Thus, the effects of IL-21 may be specific to the early stages of the autoimmune response. We found what may support this hypothesis. When we measured the serum levels of proinflammatory cytokines, such as IL-6, IL-17 and IL-23, to determine whether serum IL-21 expression correlates with systemic inflammation, we failed to detect any significant correlations (data not shown). Thus, it is possible that IL-21 is involved at the onset of autoimmune disease rather than later during the active systemic inflammation stage. In the present study, the LSG biopsies were performed for diagnostic purposes, thus the biopsy specimens are likely to be tissues from the early phase of primary SS. Since we found that IL-21 and IL-21R are expressed in these LSGs, it may be that IL-21 plays an important role in the onset and early development of primary SS.
Our data showed that IL-21 expression was prominent in infiltrating lymphocytes and ductal cells. It is not yet known whether the epithelial cells lining the duct produce IL-21. Therefore, we could not completely exclude the possibility that the high expression of IL-21 in the ductal cell is the specificity of the reagents or the artifact in the staining procedure. However, it is known that IL-21 is expressed in non-immune cells as well as in lymphocyte. IL-21 expression is enhanced at the site of the involved gut in Crohn's disease . IL-21 is also expressed in neurons in the gray matter in multiple sclerosis . This suggested the possibility that IL-21 can be produced in non-immune cells, including epithelial cells.
The glandular destruction in SS has been shown to be mediated mainly by primed CD4+ T cells . Of the lymphocytes that infiltrate the salivary glands in patients with SS, 45 to 50% are CD4+ T cells, 20% are CD8+ T cells, and about 20% are B cells . Interestingly, the confocal microscopic analyses in the present study revealed that the LSG-infiltrating cells that stained for IL-21 also stained for CXCR5. CXCR5 is a chemokine receptor that is expressed by all mature B cells as well as by a subset of antigen-experienced CD4+ T cells in lymphoid tissue . CXCR5 is strongly implicated in the follicular migration of early-activated T cells and their consequent co-localization with B cells . The priming of CD4+ T cells in lymphoid tissue result in their novel expression of CXCR5 [43, 44]. However, CXCR5 is rapidly lost during T-cell proliferation, which indicates that the follicular homing program is an early and transient stage in the T-cell activation and differentiation process [44, 45]. Continued CXCR5 expression after priming may reflect qualitative or quantitative aspects of this stimulation . Since the majority of the T cells in the salivary glands of primary SS patients are CD4+ T cells, and the IL-21 produced by activated CD4+ T cells is known to up-regulate their expression of CXCR5 , IL-21 possibly promotes the co-localization of T cells and B cells in the functional ectopic germinal centers in the salivary glands of SS patients, which in turn elevates autoantibody production. Taken together, these observations suggest that high expression of IL-21 may play an important role in the pathogenesis of primary SS by affecting both T cells and B cells.
Our present study is the first to report that there is a relationship between the elevated serum levels of IL-21 and IgG, (especially IgG1), in primary SS and that the lymphocytic infiltration in the LSGs of patients with primary SS express high levels of IL-21 along with lower levels of IL-21R. Moreover, the expression of IL-21 showed a tendency to increase as more lymphocytes infiltrated into LSGs. Further investigations on the systemic and localized effects of IL-21 in primary SS would provide a basis for targeting this molecule for customized therapy.
B lymphocyte-induced maturation protein-1
experimental autoimmune encephalitis
enzyme-linked immunosorbent assay
erythrocyte sedimentation rate
labial salivary glands
systemic lupus erythematosus
follicular helper T cells
- Th cells:
T helper cells.
This work was supported by the Happy tech. program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology 2010-0020767 and was supported by the National Research Foundation of Korea(NRF) Grant funded by the Korean Government 2005-0048538.
- Spolski R, Leonard WJ: Interleukin-21: basic biology and implications for cancer and autoimmunity. Annu Rev Immunol. 2008, 26: 57-79. 10.1146/annurev.immunol.26.021607.090316.View ArticlePubMedGoogle Scholar
- Coquet JM, Kyparissoudis K, Pellicci DG, Besra G, Berzins SP, Smyth MJ, Godfrey DI: IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 2007, 178: 2827-2834.View ArticlePubMedGoogle Scholar
- Ozaki K, Spolski R, Feng CG, Qi CF, Cheng J, Sher A, Morse HC, Liu C, Schwartzberg PL, Leonard WJ: A critical role for IL-21 in regulating immunoglobulin production. Science. 2002, 298: 1630-1634. 10.1126/science.1077002.View ArticlePubMedGoogle Scholar
- Monteleone G, Pallone F, Macdonald TT: Interleukin-21 (IL-21)-mediated pathways in T cell-mediated disease. Cytokine Growth Factor Rev. 2009, 20: 185-191. 10.1016/j.cytogfr.2009.02.002.View ArticlePubMedGoogle Scholar
- Ettinger R, Kuchen S, Lipsky PE: Interleukin 21 as a target of intervention in autoimmune disease. Ann Rheum Dis. 2008, 67 (Suppl 3): iii83-86. 10.1136/ard.2008.098400.View ArticlePubMedGoogle Scholar
- Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE: IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. J Immunol. 2007, 178: 2872-2882.View ArticlePubMedGoogle Scholar
- Ozaki K, Spolski R, Ettinger R, Kim HP, Wang G, Qi CF, Hwu P, Shaffer DJ, Akilesh S, Roopenian DC, Morse HC, Lipsky PE, Leonard WJ: Regulation of B cell differentiation and plasma cell generation by IL-21, a novel inducer of Blimp-1 and Bcl-6. J Immunol. 2004, 173: 5361-5371.View ArticlePubMedGoogle Scholar
- Moutsopoulos HM: Sjogren's Syndrom. Harrison's Principles of Internal Medicine. Edited by: Fauci AS, Braunwald E, Kasper DL, Hauser SL, Longo DL, Jameson JL, Loscalzo J. 2008, Philadelphia: McGraw-Hill, 2107-2109. 17Google Scholar
- Young DA, Hegen M, Ma HL, Whitters MJ, Albert LM, Lowe L, Senices M, Wu PW, Sibley B, Leathurby Y, Brown TP, Nickerson-Nutter C, Keith JC, Collins M: Blockade of the interleukin-21/interleukin-21 receptor pathway ameliorates disease in animal models of rheumatoid arthritis. Arthritis Rheum. 2007, 56: 1152-1163. 10.1002/art.22452.View ArticlePubMedGoogle Scholar
- Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR, Liang MH, Luthra HS, Medsger TA, Mitchell DM, Neustadt DH, Pinals RS, Schaller JG, Sharp JT, Wilder RL, Hunder GG: The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988, 31: 315-324. 10.1002/art.1780310302.View ArticlePubMedGoogle Scholar
- Hochberg MC: Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1997, 40: 1725-View ArticlePubMedGoogle Scholar
- Chisholm DM, Mason DK: Labial salivary gland biopsy in Sjogren's disease. J Clin Pathol. 1968, 21: 656-660. 10.1136/jcp.21.5.656.PubMed CentralView ArticlePubMedGoogle Scholar
- Vitali C, Bombardieri S, Jonsson R, Moutsopoulos HM, Alexander EL, Carsons SE, Daniels TE, Fox PC, Fox RI, Kassan SS, Pillemer SR, Talal N, Weisman MH, European Study Group on Classification Criteria for Sjögren's Syndrome: Classification criteria for Sjogren's syndrome: a revised version of the European criteria proposed by the American-European Consensus Group. Ann Rheum Dis. 2002, 61: 554-558. 10.1136/ard.61.6.554.PubMed CentralView ArticlePubMedGoogle Scholar
- Salomonsson S, Jonsson MV, Skarstein K, Brokstad KA, Hjelmstrom P, Wahren-Herlenius M, Jonsson R: Cellular basis of ectopic germinal center formation and autoantibody production in the target organ of patients with Sjogren's syndrome. Arthritis Rheum. 2003, 48: 3187-3201. 10.1002/art.11311.View ArticlePubMedGoogle Scholar
- Hansen A, Lipsky PE, Dorner T: New concepts in the pathogenesis of Sjogren syndrome: many questions, fewer answers. Curr Opin Rheumatol. 2003, 15: 563-570. 10.1097/00002281-200309000-00007.View ArticlePubMedGoogle Scholar
- Vogelzang A, McGuire HM, Yu D, Sprent J, Mackay CR, King C: A fundamental role for interleukin-21 in the generation of T follicular helper cells. Immunity. 2008, 29: 127-137. 10.1016/j.immuni.2008.06.001.View ArticlePubMedGoogle Scholar
- Steinmetz OM, Velden J, Kneissler U, Marx M, Klein A, Helmchen U, Stahl RA, Panzer U: Analysis and classification of B-cell infiltrates in lupus and ANCA-associated nephritis. Kidney Int. 2008, 74: 448-457. 10.1038/ki.2008.191.View ArticlePubMedGoogle Scholar
- Ettinger R, Sims GP, Fairhurst AM, Robbins R, da Silva YS, Spolski R, Leonard WJ, Lipsky PE: IL-21 induces differentiation of human naive and memory B cells into antibody-secreting plasma cells. J Immunol. 2005, 175: 7867-7879.View ArticlePubMedGoogle Scholar
- Wahren M, Ringertz NR, Pettersson I: IgM and IgG subclass distribution of human anti-Ro/SSA 60 kDa autoantibodies. Scand J Immunol. 1994, 39: 179-183. 10.1111/j.1365-3083.1994.tb03357.x.View ArticlePubMedGoogle Scholar
- Monteleone G, Pallone F, MacDonald TT: Interleukin-21: a critical regulator of the balance between effector and regulatory T-cell responses. Trends Immunol. 2008, 29: 290-294. 10.1016/j.it.2008.02.008.View ArticlePubMedGoogle Scholar
- Spolski R, Leonard WJ: The Yin and Yang of interleukin-21 in allergy, autoimmunity and cancer. Curr Opin Immunol. 2008, 20: 295-301. 10.1016/j.coi.2008.02.004.PubMed CentralView ArticlePubMedGoogle Scholar
- Korn T, Bettelli E, Gao W, Awasthi A, Jager A, Strom TB, Oukka M, Kuchroo VK: IL-21 initiates an alternative pathway to induce proinflammatory T(H)17 cells. Nature. 2007, 448: 484-487. 10.1038/nature05970.PubMed CentralView ArticlePubMedGoogle Scholar
- Yang L, Anderson DE, Baecher-Allan C, Hastings WD, Bettelli E, Oukka M, Kuchroo VK, Hafler DA: IL-21 and TGF-beta are required for differentiation of human T(H)17 cells. Nature. 2008, 454: 350-352. 10.1038/nature07021.PubMed CentralView ArticlePubMedGoogle Scholar
- Jin H, Carrio R, Yu A, Malek TR: Distinct activation signals determine whether IL-21 induces B cell costimulation, growth arrest, or Bim-dependent apoptosis. J Immunol. 2004, 173: 657-665.View ArticlePubMedGoogle Scholar
- Zeng R, Spolski R, Finkelstein SE, Oh S, Kovanen PE, Hinrichs CS, Pise-Masison CA, Radonovich MF, Brady JN, Restifo NP, Berzofsky JA, Leonard WJ: Synergy of IL-21 and IL-15 in regulating CD8+ T cell expansion and function. J Exp Med. 2005, 201: 139-148. 10.1084/jem.20041057.PubMed CentralView ArticlePubMedGoogle Scholar
- Herber D, Brown TP, Liang S, Young DA, Collins M, Dunussi-Joannopoulos K: IL-21 has a pathogenic role in a lupus-prone mouse model and its blockade with IL-21R.Fc reduces disease progression. J Immunol. 2007, 178: 3822-3830.View ArticlePubMedGoogle Scholar
- Bubier JA, Bennett SM, Sproule TJ, Lyons BL, Olland S, Young DA, Roopenian DC: Treatment of BXSB-Yaa mice with IL-21R-Fc fusion protein minimally attenuates systemic lupus erythematosus. Ann N Y Acad Sci. 2007, 1110: 590-601. 10.1196/annals.1423.063.View ArticlePubMedGoogle Scholar
- Andersson AK, Feldmann M, Brennan FM: Neutralizing IL-21 and IL-15 inhibits pro-inflammatory cytokine production in rheumatoid arthritis. Scand J Immunol. 2008, 68: 103-111. 10.1111/j.1365-3083.2008.02118.x.View ArticlePubMedGoogle Scholar
- Yuan SL, Jiang L, Zhang XL, Li SF, Duan HM, Wang XF: [Serum IL-21 level in patients with primary Sjogren's syndrome and clinical significance of IL-21]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007, 23: 124-126.PubMedGoogle Scholar
- Sawalha AH, Kaufman KM, Kelly JA, Adler AJ, Aberle T, Kilpatrick J, Wakeland EK, Li QZ, Wandstrat AE, Karp DR, James JA, Merrill JT, Lipsky P, Harley JB: Genetic association of interleukin-21 polymorphisms with systemic lupus erythematosus. Ann Rheum Dis. 2008, 67: 458-461.View ArticlePubMedGoogle Scholar
- Wang XF, Yuan SL, Jiang L, Zhang XL, Li SF, Guo Y, Wu CL, Chen JJ: [Changes of serum BAFF and IL-21 levels in patients with systemic lupus erythematosus and their clinical significance]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007, 23: 1041-1042.PubMedGoogle Scholar
- Jungel A, Distler JH, Kurowska-Stolarska M, Seemayer CA, Seibl R, Forster A, Michel BA, Gay RE, Emmrich F, Gay S, Distler O: Expression of interleukin-21 receptor, but not interleukin-21, in synovial fibroblasts and synovial macrophages of patients with rheumatoid arthritis. Arthritis Rheum. 2004, 50: 1468-1476. 10.1002/art.20218.View ArticlePubMedGoogle Scholar
- Distler JH, Jungel A, Kowal-Bielecka O, Michel BA, Gay RE, Sprott H, Matucci-Cerinic M, Chilla M, Reich K, Kalden JR, Müller-Ladner U, Lorenz HM, Gay S, Distler O: Expression of interleukin-21 receptor in epidermis from patients with systemic sclerosis. Arthritis Rheum. 2005, 52: 856-864. 10.1002/art.20883.View ArticlePubMedGoogle Scholar
- Festen EA, Goyette P, Scott R, Annese V, Zhernakova A, Lian J, Lefebvre C, Brant SR, Cho JH, Silverberg MS, Taylor KD, de Jong DJ, Stokkers PC, Mcgovern D, Palmieri O, Achkar JP, Xavier RJ, Daly MJ, Duerr RH, Wijmenga C, Weersma RK, Rioux JD: Genetic variants in the region harbouring IL2/IL21 associated with ulcerative colitis. Gut. 2009, 58: 799-804. 10.1136/gut.2008.166918.PubMed CentralView ArticlePubMedGoogle Scholar
- Liu Y, Helms C, Liao W, Zaba LC, Duan S, Gardner J, Wise C, Miner A, Malloy MJ, Pullinger CR, Kane JP, Saccone S, Worthington J, Bruce I, Kwok PY, Menter A, Krueger J, Barton A, Saccone NL, Bowcock AM: A genome-wide association study of psoriasis and psoriatic arthritis identifies new disease loci. PLoS Genet. 2008, 4: e1000041-10.1371/journal.pgen.1000041.PubMed CentralView ArticlePubMedGoogle Scholar
- Vollmer TL, Liu R, Price M, Rhodes S, La Cava A, Shi FD: Differential effects of IL-21 during initiation and progression of autoimmunity against neuroantigen. J Immunol. 2005, 174: 2696-2701.View ArticlePubMedGoogle Scholar
- Monteleone G, Monteleone I, Fina D, Vavassori P, Del Vecchio Blanco G, Caruso R, Tersigni R, Alessandroni L, Biancone L, Naccari GC, MacDonald TT, Pallone F: Interleukin-21 enhances T-helper cell type I signaling and interferon-gamma production in Crohn's disease. Gastroenterology. 2005, 128: 687-694. 10.1053/j.gastro.2004.12.042.View ArticlePubMedGoogle Scholar
- Tzartos JS, Craner MJ, Friese MA, Jakobsen KB, Newcombe J, Esiri MM, Fugger L: IL-21 and IL-21 receptor expression in lymphocytes and neurons in multiple sclerosis brain. Am J Pathol. 2011, 178: 794-802. 10.1016/j.ajpath.2010.10.043.PubMed CentralView ArticlePubMedGoogle Scholar
- Xanthou G, Tapinos NI, Polihronis M, Nezis IP, Margaritis LH, Moutsopoulos HM: CD4 cytotoxic and dendritic cells in the immunopathologic lesion of Sjogren's syndrome. Clin Exp Immunol. 1999, 118: 154-163. 10.1046/j.1365-2249.1999.01037.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Skopouli FN, Fox PC, Galanopoulou V, Atkinson JC, Jaffe ES, Moutsopoulos HM: T cell subpopulations in the labial minor salivary gland histopathologic lesion of Sjogren's syndrome. J Rheumatol. 1991, 18: 210-214.PubMedGoogle Scholar
- King C, Tangye SG, Mackay CR: T follicular helper (TFH) cells in normal and dysregulated immune responses. Annu Rev Immunol. 2008, 26: 741-766. 10.1146/annurev.immunol.26.021607.090344.View ArticlePubMedGoogle Scholar
- Ebert LM, Schaerli P, Moser B: Chemokine-mediated control of T cell traffic in lymphoid and peripheral tissues. Mol Immunol. 2005, 42: 799-809. 10.1016/j.molimm.2004.06.040.View ArticlePubMedGoogle Scholar
- Legler DF, Loetscher M, Roos RS, Clark-Lewis I, Baggiolini M, Moser B: B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5. J Exp Med. 1998, 187: 655-660. 10.1084/jem.187.4.655.PubMed CentralView ArticlePubMedGoogle Scholar
- Schaerli P, Loetscher P, Moser B: Cutting edge: induction of follicular homing precedes effector Th cell development. J Immunol. 2001, 167: 6082-6086.View ArticlePubMedGoogle Scholar
- Langenkamp A, Nagata K, Murphy K, Wu L, Lanzavecchia A, Sallusto F: Kinetics and expression patterns of chemokine receptors in human CD4+ T lymphocytes primed by myeloid or plasmacytoid dendritic cells. Eur J Immunol. 2003, 33: 474-482. 10.1002/immu.200310023.View ArticlePubMedGoogle Scholar
- Ansel KM, McHeyzer-Williams LJ, Ngo VN, McHeyzer-Williams MG, Cyster JG: In vivo-activated CD4 T cells upregulate CXC chemokine receptor 5 and reprogram their response to lymphoid chemokines. J Exp Med. 1999, 190: 1123-1134. 10.1084/jem.190.8.1123.PubMed CentralView ArticlePubMedGoogle Scholar
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