Volume 14 Supplement 1

Proceedings of the 8th Global Arthritis Research Network (GARN) Meeting and 1st Bio-Rheumatology International Congress (BRIC)

Open Access

Association between serum level of Vitamin D with autoantibodies expression, disease activity (SLEDAI) and bone mineral density (BMD) in patients with Systemic Lupus Erythematosus (SLE)

  • Handono Kalim1,
  • Singgih Wahono1,
  • Putra Suryana BP1,
  • Lenny Puspitasari1,
  • Fajar Hadi Wijayanto1 and
  • Kusworini Handono2
Arthritis Research & Therapy201214(Suppl 1):P23

DOI: 10.1186/ar3624

Published: 29 February 2012


Vitamin D defficiency has been reported to have negative association with clinical manifestation and disease activity of SLE [1, 2]. Vit D has an important role in the pathogenesis of SLE [3] and it is necessary to give vit D supplementation to the patients [4]. The objective of our study was to determine the association between serum vitamin D level with auto antibodies expression, disease activity and bone mineral density in SLE patients.

Patients and methods

55 female patients with SLE were recruited from Clinic of Rheumato-Immunology, Saiful Anwar Hospital, Malang, Indonesia. Mean age of the patients 31.12 years (12-64 yo) with duration of illness 18,4 months (2-54 mo). Serum vitamin (25 (OH)D3 level was assayed using ELISA method (Cusabio, normal value>30 ng/mL). Anti ds-DNA and Anti Cardiolipin antibodies were assayed using ELISA method (Diagnostic Automation, Inc, USA). Disease activity assessed by SLE disease activity index (SLEDAI) and BMD was assessed by bone densitometry using DEXA. Association between variables (serum vitamin D and autoantibodies level, BMD and SLEDAI) were analyzed using Spearman correlation.


The mean of serum 25(OH)D3 level was 22.80 ± 16,23 ng/mL.14 patients (25.5%) had vitamin D deficiency (<10 ng/mL), 34 patients (61.8%) had vitamin D insufficiency (10-30 ng/mL), and 7 patients (14.7%) had normal vitamin D levels. There were significant difference level of anti-dsDNA antibodies (112.46 vs 267.13 U/ml; p < 0.05) and IgM ACA (16.40 vs 29.7 IU/ml; p < 0,05) in patients with vitamin D insufficiency and vitamin D defisiency. Serum level of 25(OH)D3 were negatively related with level of anti-dsDNA and IgM ACA (r: - 0, and r: - 0,72 respectively). The mean of SLEDAI was 15,0 + 10.46. Serum vitamin D levels were inversely correlated with SLEDAI (R=-0.319, p<0.05). Normal BMD at lumbal spine found in 21 (38.2%) patients. 26 patients (47.3%) were osteopenia, and 8 (14.5%) patients were osteoporosis. At femoral neck, 25 (45.5%) patients had normal BMD, 23(41.8%) patients were osteopenia, 7 (12.7%) patients were osteoporosis. There were no significant correlation between vitamin D level and BMD at lumbal spine (p = 0,531) and at femoral neck (p = 0,175).


A large proportion of SLE patients had low vitamin D levels. There were positive association between vit D level and autoantibodies expression in SLE and negative association between serum vitamin D levels with SLEDAI. No association was found between serum vit D level and BMD.

Authors’ Affiliations

Rheumato-Immunology Division, Department of Internal Medicine, Brawijaya University
Department of Clinical Pathology Faculty of Medicine, Brawijaya University


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© Kalim et al.; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.