Volume 14 Supplement 1

Proceedings of the 8th Global Arthritis Research Network (GARN) Meeting and 1st Bio-Rheumatology International Congress (BRIC)

Open Access

Active repression by Blimp1 play an important role in osteoclast differentiation

  • Keizo Nishikawa1,
  • Tomoki Nakashima2, 3, 4,
  • Mikihito Hayashi2, 3, 4,
  • Takanobu Fukunaga2, 3, 4,
  • Shigeaki Kato5, 6,
  • Tatsuhiko Kodama7,
  • Satoru Takahashi8,
  • Kathryn Calame9 and
  • Hiroshi Takayanagi2, 3, 4
Arthritis Research & Therapy201214(Suppl 1):P54

DOI: 10.1186/ar3655

Published: 29 February 2012

Regulation of irreversible cell lineage commitment depends on a delicate balance between positive and negative regulators, which comprise a sophisticated network of transcription factors. Receptor activator of nuclear factor-κB ligand (RANKL) stimulates the differentiation of bone-resorbing osteoclasts through the induction of nuclear factor of activated T-cells c1 (NFATc1), the essential transcription factor for osteoclastogenesis. Osteoclast-specific robust induction of NFATc1 is achieved through an autoamplification mechanism, in which NFATc1 is constantly activated by calcium signaling while the negative regulators of NFATc1 are being suppressed. However, it has been unclear how such negative regulators are repressed during osteoclastogenesis. Here we show that B lymphocyte-induced maturation protein-1 (Blimp1; encoded by Prdm1), which is induced by RANKL through NFATc1 during osteoclastogenesis, functions as a transcriptional repressor of anti-osteoclastogenic genes such as Irf8 and Mafb. Overexpression of Blimp1 leads to an increase in osteoclast formation and Prdm1-deficient osteoclast precursor cells do not undergo osteoclast differentiation efficiently. The importance of Blimp1 in bone homeostasis is underscored by the observation that mice with an osteoclast-specific deficiency in the Prdm1 gene exhibit a high bone mass phenotype owing to a decreased number of osteoclasts. Thus, NFATc1 choreographs the cell fate determination of the osteoclast lineage by inducing the repression of negative regulators as well as its effect on positive regulators.

Authors’ Affiliations

Laboratory of Cellular Dynamics Immunology Frontier Research Center, Osaka University
Department of Cell Signaling, Graduate School, Tokyo Medical and Dental University
Global Center of Excellence Program, International Research Center for Molecular Science in Tooth and Bone Diseases
Japan Science and Technology Agency, ERATO, TakayanagiOsteonetwork Project
Institute of Molecular and Cellular Biosciences, Graduate School of Medicine, University of Tokyo
Japan Science and Technology Agency, ERATO
Department of Molecular Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo
Institute of Basic Medical Sciences and Laboratory Animal Resource Center, University of Tsukuba
Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons


© Nishikawa et al.; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.