Correction: FoxP3 and Bcl-xL cooperatively promote regulatory T cell persistence and prevention of arthritis development

  • Rizwanul Haque1,

    Affiliated with

    • Fengyang Lei1,

      Affiliated with

      • Xiaofang Xiong1,

        Affiliated with

        • Yuzhang Wu2 and

          Affiliated with

          • Jianxun Song1, 2Email author

            Affiliated with

            Arthritis Research & Therapy201214:401

            DOI: 10.1186/ar3790

            Published: 10 April 2012

            Correction

            Following publication of our recent article [1], it was brought to our attention that there are errors in Figure 5b and Supplementary Table 2.

            The corrected Figure and table are given here as Figure 1 (a corrected version of Figure 5b [1]) and Table 1 (a corrected version of Supplementary Table 2 [1]).
            http://static-content.springer.com/image/art%3A10.1186%2Far3790/MediaObjects/13075_2012_3532_Fig1_HTML.jpg
            Figure 1

            Adoptive cell transfer of FoxP3- and Bcl-xL-transduced regulatory T cells suppresses collagen-induced arthritis (CIA).

            Table 1

            Summary of the mouse arthritis scores of the three experiments

              

            Score

             

            Days (post immunization)

            Mig

            Mig-FoxP3

            Mig-Bcl-xL-2A-FoxP3

            10

            0, 0, 0

            0, 0, 0

            0, 0, 0

            20

            0, 0, 0

            0, 0, 0

            0, 0, 0

            22

            1.00, 0, 0.83

            0, 0, 0

            0, 0, 0

            24

            2.00, 0, 1.67

            0, 0, 0

            0, 0, 0

            28

            2.67, 1.67, 2.67

            0.50, 0, 0.50

            0, 0, 0

            30

            3.30, 2.83, 3.30

            0.50, 0, 1.17

            0, 0, 0

            32

            3.67, 3.00, 3.50

            0.50, 1.17, 1.50

            0, 0, 0

            34

            3.83, 3.00, 3.67

            1.00, 1.67, 1.50

            0.83, 0, 0

            38

            3.83, 3.00, 3.67

            1.17, 1.67, 1.83

            0.83, 0.50, 0

            40

            3.83, 3.30, 3.67

            1.50, 1.67, 2.00

            1.00, 0.50, 0

            42

            3.83, 3.30, 3.83

            1.50, 2.00, 2.17

            1.17, 0.50, 0.50

            48

            3.83, 3.50, 3.83

            2.00, 2.00, 2.67

            1.50, 0.83, 0.83

            50

            3.83, 3.50, 3.83

            2.00, 2.17, 2.67

            1.67, 1.00, 1.00

            60

            3.83, 3.50, 3.83

            2.00, 2.40, 3.00

            1.67, 1.00, 1.17

            Naive CD4+ T cells from DBA/1J mice were stimulated with anti-CD3 plus anti-CD28 antibodies. On days 2 and 3, the cells were transduced with retroviral constructs: vector (Mig), FoxP3 (Mig-FoxP3), or FoxP3 with Bcl-xL (Mig-Bcl-xL-2A-FoxP3). On day 6, green fl uorescent protein-positive (GFP+) T cells were sorted and prepared for adoptive cell transfer. CIA was induced in male DBA/1J mice (>4 months old) by one (day 0) intradermal immunization in the base of the tail with 100 μg of bovine type II collagen in complete Freund's adjuvant, containing 5 mg/mL killed Mycobacterium tuberculosis (H37Ra). On day 15 after the immunization, the mice received transduced GFP+ cells (2.5 × 106 per mouse, six mice per group). In the following days, the arthritis clinical score was evaluated by examining the paws and using a 4-point scale: 0, normal paw; 1, minimal swelling or redness; 2, redness and swelling involving the entire forepaw; 3, redness and swelling involving the entire limp; 4, joint deformity or ankylosis or both. Values are the mean ± standard error of the mean of data obtained in three experiments, and in each experiment, six mice per group were used.

            Authors’ Affiliations

            (1)
            Department of Microbiology & Immunology and Penn State Hershey Cancer Institute, The Pennsylvania State University College of Medicine
            (2)
            Institute of Immunology, The Third Military Medical University

            References

            1. Haque R, Lei F, Xiong X, Wu Y, Song J: FoxP3 and Bcl-xL cooperatively promote regulatory T cell persistence and prevention of arthritis development. Arthritis Res Ther 2010, 12:R66.PubMedView Article

            Copyright

            © BioMed Central Ltd 2012

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