Enhanced susceptibility to lipopolysaccharide-induced arthritis and endotoxin shock in interleukin-32 alpha transgenic mice through induction of tumor necrosis factor alpha
© Nakayama et al.; licensee BioMed Central Ltd. 2011
Received: 19 December 2011
Accepted: 21 May 2012
Published: 21 May 2012
The present study assessed the potential functions of interleukin (IL)-32α on inflammatory arthritis and endotoxin shock models using IL-32α transgenic (Tg) mice. The potential signaling pathway for the IL-32-tumor necrosis factor (TNF)α axis was analyzed in vitro.
IL-32α Tg mice were generated under control of a ubiquitous promoter. Two disease models were used to examine in vivo effects of overexpressed IL-32α: Toll-like receptor (TLR) ligand-induced arthritis developed using a single injection of lipopolysaccharide (LPS) or zymosan into the knee joints; and endotoxin shock induced with intraperitoneal injection of LPS and D-galactosamine. TNFα antagonist etanercept was administered simultaneously with LPS in some mice. Using RAW264.7 cells, in vitro effects of exogenous IL-32α on TNFα, IL-6 or macrophage inflammatory protein 2 (MIP-2) production were assessed with or without inhibitors for nuclear factor kappa B (NFκB) or mitogen-activated protein kinase (MAPK).
Single injection of LPS, but not zymosan, resulted in development of severe synovitis with substantial articular cartilage degradation in knees of the Tg mice. The expression of TNFα mRNA in inflamed synovia was highly upregulated in the LPS-injected Tg mice. Moreover, the Tg mice were more susceptive to endotoxin-induced lethality than the wild-type control mice 48 hours after LPS challenge; but blockade of TNFα by etanercept protected from endotoxin lethality. In cultured bone marrow cells derived from the Tg mice, overexpressed IL-32α accelerated production of TNFα upon stimulation with LPS. Of note, exogenously added IL-32α alone stimulated RAW264.7 cells to express TNFα, IL-6, and MIP-2 mRNAs. Particularly, IL-32α -induced TNFα, but not IL-6 or MIP-2, was inhibited by dehydroxymethylepoxyquinomicin (DHMEQ) and U0126, which are specific inhibitors of nuclear factor kappa B (NFκB) and extracellular signal regulated kinase1/2 (ERK1/2), respectively.
These results show that IL-32α contributed to the development of inflammatory arthritis and endotoxin lethality. Stimulation of TLR signaling with LPS appeared indispensable for activating the IL-32α-TNFα axis in vivo. However, IL-32α alone induced TNFα production in RAW264.7 cells through phosphorylation of inhibitor kappa B (IκB) and ERK1/2 MAPK. Further studies on the potential involvement of IL-32α-TNFα axis will be beneficial in better understanding the pathology of autoimmune-related arthritis and infectious immunity.
Interleukin-32 (IL-32) was originally identified as natural killer (NK) transcript 4, which is induced by IL-18 in NK cells . NK transcript 4 showed cytokine-like characteristics and played a critical role in inflammation and was therefore renamed IL-32. This cytokine is reportedly produced by NK cells, T cells, epithelial cells, monocytes, and fibroblasts after stimulation by IL-2, IL-12, and IL-18 and interferon-gamma . Initially, four isoforms of IL-32 (IL-32α, β, γ, and δ) derived from alternative splicing of a single gene. Among these, IL-32α is the shortest transcript, whereas IL-32 γ is the longest isoform and has the strongest biological activity [2, 3]. Two additional isoforms, IL-32ε and ζ, have recently been identified, but these isoforms are not ubiquitously expressed except in T cells .
IL-32 has been shown to exhibit properties typical of a proinflammatory cytokine and to drive the induction of other proinflammatory cytokines and chemokines, such as tumor necrosis factor-alpha (TNFα) and IL-1, IL-6, and IL-8. Owing to such proinflammatory properties, IL-32 has been considered to play a key role in the development of various inflammatory diseases, including rheumatoid arthritis (RA), inflammatory bowel disease , mycobacterial [6, 7] or viral [8–10] infection, chronic obstetric pulmonary disease , and pancreatic tumor [12, 13]. Although no receptor or analog of IL-32 has yet been identified in mice, human IL-32 reportedly exerts proinflammatory effects as an inducer of TNFα and other inflammatory cytokines in mice both in vitro and in vivo [2, 14–16].
During the last decade, TNFα and IL-6 became widely perceived as substantial therapeutic targets in RA given that the use of either anti-TNFα or anti-IL-6 therapy could successfully control chronic inflammation in RA. As IL-32 is capable of inducing TNFα and IL-6, this cytokine is increasingly becoming a focus as a potential therapeutic target in RA and other inflammatory disorders. Mounting evidence regarding upstream signaling regulators for IL-32 production has been accumulating in the literature [12, 17–20]. However, signaling pathways that are downstream of IL-32 and that lead to TNFα production have yet to be fully elucidated. Most investigators advocate the position that IL-32 augments Toll-like receptor (TLR) signaling, and TLR-2, -3, and -4 are associated with the effects of IL-32 signaling, although the detailed mechanisms remain to be clarified. Only a few studies to date have reported the implications of mitogen-activated protein kinase (MAPK) or nuclear factor kappa B (NF-κB) pathways in IL-32 signaling [2, 21–23].
The present study generated IL-32α transgenic (Tg) mice that overexpressed human IL-32α under a control of ubiquitous CAG promoter, and it assessed the in vivo effects of IL-32α on TLR signaling in the induction of arthritis and endotoxin shock models using the Tg mice. In addition, the potential signaling pathway of the IL-32α-TNFα axis was analyzed in vitro.
Materials and methods
Lipopolysaccharide (LPS) from Escherichia coli 0111:B4 and zymosan A from Saccharomyces cerevisiae were purchased from Sigma-Aldrich (St. Louis, MO, USA), and D-galactosamine was purchased from Wako Pure Chemical Industries (Osaka, Japan). Etanercept was obtained from Wyeth (Tokyo, Japan). Recombinant human IL-32α protein (rIL-32α) was obtained from Takara Bio (Shiga, Japan). IL-32α-specific enzyme-linked immunosorbent assay (ELISA) (Human IL-32α ELISA MAX Deluxe Set) was purchased from BioLegend (San Diego, CA, USA), and TNFα-specific ELISA (Quantikine Mouse TNFα) and anti-IL-32α antibody were purchased from R&D Systems (Minneapolis, MN, USA). All other antibodies were purchased from Cell Signaling Technology Japan (Tokyo, Japan). Dehydroxymethylepoxyquinomicin (DHMEQ) (an inhibitor for NF-κB) was provided as previously described [24, 25]. MAPK inhibitors U0126, SB203580, and SP600125 - which are inhibitors for ERK1/2, p38, and JNK, respectively - were purchased from Sigma-Aldrich. DHMEQ and MAPK inhibitors were dissolved in 100% dimethyl sulfoxide (DMSO) at 100 mg/mL and were stored in aliquots at -30°C. Before use in cell culture, they were diluted with the medium to a final DMSO concentration of not more than 0.05%.
Real-time polymerase chain reaction analysis
Primers used in real-time polymerase chain reaction
Mice organs or cell lysates were prepared by using RIPA buffer: 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 150 mM NaCl, 10 mM Tris-HCl, pH 7.5, 5 mM ethylenediaminetetraacetic acid (EDTA), and a protease inhibitor cocktail. An equal volume of protein from each lysate was separated by SDS-PAGE, and separated proteins were transferred to nitrocellulose membranes. After transfer, the membrane was blocked with 5% non-fat dried skim milk for 1 hour at room temperature and then incubated with the primary antibody against the target molecule overnight. Next, the membrane was incubated with horseradish peroxidase-conjugated secondary antibody for 1 hour at room temperature. After washing, protein was detected by using an enhanced chemiluminescence (ECL) system. The same membrane was then stripped by Tris-HCL (62.5 mM), 2% SDS, and β-mercaptoethanol and blocked with skim milk and incubated with primary antibody against another molecule, following the same procedure mentioned above.
Generation of human IL-32α transgenic mice
We generated IL-32α Tg mice, which overexpressed human IL-32α under a control of a ubiquitous CAG promoter constructed by the first intron of the chicken β-actin gene and a portion of the rabbit β-globin gene . The background of these mice was C57BL/6 Jcl (obtained from CLEA Japan, Tokyo, Japan). Wild-type C57BL/6 Jcl (Wt) mice were also obtained from CLEA Japan. All mice were 18 to 20 weeks old when they were used. All animal experiments were conducted in accordance with institutional and national guidelines. IL-32α insertion was confirmed by amplification of the genome DNA isolated from mouse organs by using real-time PCR. The reason real-time PCR was employed was that we wanted to simultaneously detect and quantify transgene-derived IL-32α in multiple organs of Tg mice. The amount of TNFα expression in multiple organs was measured by real-time PCR. IL-32α protein in multiple organs was detected by immunoblot analysis. Levels of IL-32α in blood serum, knee, and liver lysate were measured by specific ELISA.
Inflammatory arthritis model
Inflammatory arthritis was induced as described previously [27–29]. As in these reports, intra-articular injection of LPS was employed in IL-32α Tg mice to elucidate the arthritogenic capacity of IL-32α. Knees of Tg and Wt mice were injected once with LPS (1 μg, much less than the reported amount [27, 28]) or zymosan (20 μg, much less than the reported amount ) without any other material as a booster. Injection of phosphate-buffered saline (PBS) served as a control to the contralateral knee of the same mouse. Two weeks after injection, a histopathological examination was performed, and TNFα mRNA expression in synovia of knees was quantitatively measured by using real-time PCR.
Endotoxin shock model
Endotoxin shock was induced by intraperitoneal injections of 5 μg of LPS and 10 mg of D-galactosamine . Half of the mice were injected with 100 μg of etanercept at the same time. All injected mice were closely monitored every hour for the first 16 hours and every 3 to 6 hours thereafter for 48 hours. In an additional experiment, we sacrificed mice at 1, 3, and 6 hours after intraperitoneal injection and extracted the liver, spleen, and blood serum. TNFα mRNA of liver and spleen lysates was measured by real-time PCR, and serum TNFα was measured by ELISA.
Bone marrow (BM) was extracted from the leg bones of Tg or Wt mice and cultured for 72 hours in alpha-minimum essential medium (Sigma-Aldrich) containing 10% fetal calf serum (FCS), 100 U/mL penicillin, 100 mg/mL streptomycin, and 1% Gluta Max (Gibco Invitrogen, Carlsbad, CA, USA) supplemented with macrophage colony-stimulating factor (50 ng/mL; Kyowa Hakko Kirin, Tokyo, Japan). RAW 264.7 cells were cultured in Dulbecco's modified Eagle's medium (high-glucose) (Gibco Invitrogen) containing 10% FCS, 100 U/mL penicillin, and 100 μg/mL streptomycin.
Stimulation of cells
BM cells from Tg and Wt mice (106 cells) were incubated under stimulation of LPS (50 ng/mL) for 1, 3, 6, and 24 hours, and the amount of TNFα was measured by real-time PCR. TNFα concentration in culture media of BM cells (105 cells) after 24-hour incubation with LPS (5, 10, or 50 ng/mL) was measured by specific ELISA. RAW 264.7 cells (2 × 104 cells) were cultured with rIL-32α or LPS for 24 hours, and the TNFα concentration in culture media was measured by ELISA. For the analysis of signaling pathway, RAW 264.7 cells (2 × 104 cells) were stimulated with rIL-32α in combination with the specific inhibitors of NF-κB and MAPKs, such as DHMEQ (0.4, 2, or 10 μg/mL), U0126 (0.2, 1, or 5 μM), SB203580 (0.2, 1, or 5 μM), and SP600125 (0.4, 2, or 10 μM). DMSO (final concentration of 0.05%) served as a control. After 24 hours of culture, the level of TNFα in culture media was measured by ELISA. For immunoblot analysis, RAW 264.7 cells (2 × 106 cells) were cultured with rIL-32α for 5, 10, 30, 60, 90, 120, or 180 minutes, followed by washing with ice-cold PBS and lysis in RIPA detergent buffer. The resultant cell lysates were then immunoblotted by using affinity-purified antibodies against phospho-IκB, IκB, phospho-ERK1/2, ERK1/2, phospho-p38, p38, phospho-JNK, JNK, and β-actin. mRNA expressions for IL-6 and macrophage inflammatory protein 2 (MIP-2) as well as TNFα in IL-32-stimulated RAW 264.7 cells (2 × 104 cells) were measured by real-time PCR, and the inhibitory effects of specific signaling inhibitors, including DHMEQ, U0126, SB203580, and SP600125, were analyzed.
Results are reported as the mean ± standard deviation. Statistical analysis was undertaken by using a two-tailed Student t test. Differences were considered statistically significant at a P value of less than 0.05. The endotoxin shock model was graphed in Kaplan-Meier format and analyzed by a log-rank test. All experiments were performed in three or four times.
Generation of human IL-32α transgenic mice
Single intra-articular injection of LPS, but not zymosan, induced inflammatory synovitis and cartilage degradation in transgenic mice
Transgenic mice exhibited severe endotoxin lethality after LPS challenge
Endogenously overexpressed IL-32α accelerated production of TNFα upon stimulation with LPS
Exogenous IL-32α enhanced TNFα production in RAW 264.7 cells through NF-κB and ERK1/2 signaling pathways
Exogenous IL-32α stimulated IL-6 and MIP-2 expression in RAW 264.7 cells independently of NF-κB and MAPK signaling pathways
To date, the arthritogenic role of IL-32 has been elucidated on the basis of accumulated evidence that overexpression of IL-32β in a mouse model using bone transplantation exacerbated collagen-induced arthritis in mice  and that intra-articular injection of IL-32γ in mouse knee joints resulted in severe joint inflammation . In this study, although IL-32α Tg mice did not spontaneously exhibit any abnormal phenotype, intra-articular injection of low-dose LPS resulted in the development of inflammatory arthritis. However, injection of zymosan was not capable of sufficiently inducing TNFα and subsequent arthritis. As LPS is known as a specific ligand of TLR-4, interaction of IL-32α with TLR-4 may play a critical role in the development of arthritis, and this was also the case in LPS-triggered endotoxin shock in the Tg mice. This endotoxin shock model provided an excellent means to evaluate the effects of IL-32α on infectious immunity. In the present study, IL-32α overproduction in Tg mice was associated with severe endotoxin lethality; this was shown to be mediated through the induction of TNFα, because etanercept significantly attenuated the endotoxin shock.
Although the present study clearly demonstrated that LPS, as a TLR-4 agonist, but not the TLR-2 agonist zymosan, might play a key role in potentiating the proinflammatory activity of IL-32α, how exactly IL-32α interacted with the TLR-4 signaling pathway remains unclear. Most recently, Heinhuis and colleagues  reported that LPS co-stimulation was mandatory to elicit IL-32 bioactivity in THP-1 cells, and the present study obtained similar findings that TNFα production promoted by IL-32α required co-stimulation with LPS (Figure 4). In terms of the interaction between IL-32 and TLR-2/NOD2 (nucleotide-binding oligomerization domain-containing protein 2) signaling, IL-32 has been reported to stimulate TNFα, IL-6, and IL-8 production by directly increasing expression of TLR-2 and NOD2 . Conversely, the interaction of IL-32 with TLR-4 can be speculated to involve the binding of IL-32 to its putative receptor modulates downstream signaling for TLR-4 or other TLRs, since the proinflammatory activities of IL-32 were present even in macrophages derived from TLR-4-/- mice, and stimulation with IL-32 plus TLR ligand elicited only additive effects rather than synergistic effects . Two candidate molecules potentially connecting IL-32α and TLR-4 signaling are considered. One is proteinase-3 and the other is proteinase-activated receptor 2 (PAR2); the former reportedly acts as an IL-32-binding protein and cleaves all isoforms of IL-32 to generate a more active form [34, 35], and the latter has been shown to be associated with late NF-κB activation and subsequent TNFα production predominantly through a myeloid differentiation factor 88 (MyD88)-independent pathway .
In contrast to mounting evidence on upstream signaling regulators for IL-32, downstream signaling pathways of IL-32 toward TNFα production have not yet been analyzed in detail, and only a small number of reports have focused on different signals in different cell types. The first report of IL-32 advocated that IL-32α stimulated TNFα production through activation of NF-κB and p38 MAPK in mouse RAW 267.4 cells; the two peaks of p38 MAPK phosphorylation at 5 and 45 minutes were considered a characteristic finding for this cytokine . Netea and colleagues  subsequently reported that IL-32-induced TNFα production by human peripheral blood mononuclear cells (PBMCs) was similarly regulated through phosphorylation of p38 MAPK. On the other hand, ERK1/2 MAPK was dominant in IL-32-induced osteoclastogenesis for human PBMCs  and in IL-32-induced IL-6 and IL-8 production by human fibroblast-like synoviocytes . The present study demonstrated that IL-32α-induced TNFα production was mediated through phosphorylation of IκB and ERK1/2 in RAW 267.4 cells. Actually, all three components of MAPKs - p38, JNK, and ERK1/2 - were constitutively phosphorylated in RAW 267.4 cells; however, only ERK1/2 phosphorylation was significantly accelerated in response to IL-32α stimulation. This observation corroborated the fact that the addition of inhibitors for ERK1/2 and NF-κB suppressed each phosphorylation (data not shown and Figure S1 of Additional file 1) and consequently canceled IL-32α-induced upregulation of TNFα at the mRNA level (Figures 5b and 6). Given the delayed phosphorylation of IκB and ERK1/2 starting at 30 minutes in this study, IL-32α in RAW 267.4 cells might not directly activate IκB or ERK1/2. Instead, other molecules might play an important role in IκB or ERK1/2 activation of IL-32α, or the IL-32α-TNFα axis might use the MyD88-independent pathway reportedly associated with the late inflammatory response of TLR-4 . Our study also revealed that IL-32α induced IL-6 and MIP-2 as well as TNFα, but their induction was not canceled by inhibitors for NF-κB or MAPKs. This observation indicates that a signaling pathway other than NF-kB or MAPKs might be involved in IL-6 and MIP-2 expressions.
IL-32γ Tg mice obtained by using a promoter similar to that of the present study reportedly exhibited no apparent phenotype, but once inflammatory colitis was induced with dextran sodium sulfate (DSS) in the Tg mice, severe colitis occurred within 4 days . Interestingly, at more than 6 days after DSS challenge, the degree of colonic inflammation in the Tg mice was significantly reduced, and recovery was more rapid than that in Wt mice because of increased IL-10 levels in serum. In another study, IL-32β was reported to promote the production of IL-10 in human cell lines . According to our data on RAW 264.7 cells, the level of TNFα in culture media peaked at 12 hours after stimulation with IL-32α and gradually decreased thereafter, whereas IL-10 levels increased from 24 to 96 hours after stimulation (data not shown and Figure S2 of Additional file 2). IL-32 is thus considered to represent a cytokine possessing contradictory properties according to the different phases of the disease. Such paradoxical effects of IL-32 were not observed in our Tg mice. In fact, a single intra-articular injection of LPS in our Tg mice resulted in a transient flare of inflammatory arthritis, characterized by neutrophil infiltration and synovial proliferation, but such inflammation might cease within 2 weeks, followed by amelioration of synovitis with only mild cartilage erosion remaining. On the other hand, the endotoxin shock model using our Tg mice was suitable for examining short-term effects, but not long-term effects, of IL-32 in vivo since most mice died within several hours after LPS challenge, and TNFα induced by IL-32α and LPS was confirmed as an early mediator of endotoxin lethality . The time-dependent and complicated regulation of IL-32 and the relevant molecules of the IL-32-TNFα axis during the course of autoimmune-related arthritis and infectious immunity should be elucidated in future studies.
This study revealed that IL-32α contributed to the development of LPS-induced inflammatory arthritis and endotoxin lethality; therefore, stimulation of LPS appears indispensable for activating the IL-32-TNFα axis in vivo. However, IL-32α alone induced TNFα production in RAW 264.7 cells through phosphorylation of IκB and ERK1/2 MAPK.
dextran sodium sulfate
enzyme-linked immunosorbent assay
extracellular signal regulated kinase1/2
fetal calf serum
inhibitor kappa B
mitogen-activated protein kinase
macrophage inflammatory protein
myeloid differentiation factor 88
nuclear factor kappa B
nucleotide-binding oligomerization domain-containing protein
peripheral blood mononuclear cell
polymerase chain reaction
recombinant human interleukin-32α protein
tumor necrosis factor
wild-type (C57BL/6 Jcl).
This work was partially supported by Grants-in-aid for Scientific Research (C) (#21591959) from the Japan Society for the Promotion of Science.
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