Open Access

Analysis of the association between CD40 and CD40 ligand polymorphisms and systemic sclerosis

  • María Teruel1Email author,
  • Carmen P Simeon2,
  • Jasper Broen3,
  • Madelon C Vonk3,
  • Patricia Carreira4,
  • Maria Teresa Camps5,
  • Rosa García-Portales6,
  • Esmeralda Delgado-Frías7,
  • Maria Gallego8,
  • Gerard Espinosa9,
  • the Spanish Scleroderma Group10,
  • Lorenzo Beretta11,
  • Paolo Airó12,
  • Claudio Lunardi13,
  • Gabriela Riemekasten14,
  • Torsten Witte15,
  • Thomas Krieg16,
  • Alexander Kreuter17,
  • Jörg HW Distler18,
  • Nicolas Hunzelmann16,
  • Bobby P Koeleman19,
  • Alexandre E Voskuyl20,
  • Annemie J Schuerwegh21,
  • Miguel Ángel González-Gay22,
  • Timothy RDJ Radstake23 and
  • Javier Martin1
Arthritis Research & Therapy201214:R154

DOI: 10.1186/ar3890

Received: 16 March 2012

Accepted: 25 June 2012

Published: 25 June 2012

Abstract

Introduction

The aim of the present study was to investigate the possible role of CD40 and CD40 ligand (CD40LG) genes in the susceptibility and phenotype expression of systemic sclerosis (SSc).

Methods

In total, 2,670 SSc patients and 3,245 healthy individuals from four European populations (Spain, Germany, The Netherlands, and Italy) were included in the study. Five single-nucleotide polymorphisms (SNPs) of CD40 (rs1883832, rs4810485, rs1535045) and CD40LG (rs3092952, rs3092920) were genotyped by using a predesigned TaqMan allele-discrimination assay technology. Meta-analysis was assessed to determine whether an association exists between the genetic variants and SSc or its main clinical subtypes.

Results

No evidence of association between CD40 and CD40LG genes variants and susceptibility to SSc was observed. Similarly, no significant statistical differences were observed when SSc patients were stratified by the clinical subtypes, the serologic features, and pulmonary fibrosis.

Conclusions

Our results do not suggest an important role of CD40 and CD40LG gene polymorphisms in the susceptibility to or clinical expression of SSc.

Introduction

Systemic sclerosis (SSc) is an autoimmune disease of the connective tissue characterized by excessive fibrosis of the dermis and vascular damage. It also affects internal organs, such as the lung, gastrointestinal, and vascular systems [1]. SSc is a complex polygenic disease in which environmental and genetic factors are involved in the susceptibility to this disease. Candidate gene and genome-wide association studies (GWASs) performed in SSc have identified new loci implicated in the susceptibility to SSc [2]. Nevertheless, the complete genetic components of SSc remain unknown.

CD40 is a member of the tumor necrosis factor receptor superfamily (TNFR), and it is expressed on the surface of several immune and nonhematopoietic cells, such as B cells, macrophages, dendritic cells, fibroblasts, and endothelial cells in certain pathogenic conditions [3]. Its ligand, CD40LG (CD154), is expressed mainly on the surface of CD4+ T cells. CD40-CD40LG interactions are necessary for the activation of both humoral and cellular immune responses [3]. The CD40-CD40LG pathway has been suggested to play an important role in the pathogenesis of autoimmune diseases [4]. An increase of soluble CD40LG (sCD40LG) has been observed in many autoimmune diseases, such as systemic lupus erythematosus (SLE) [5], rheumatoid arthritis (RA) [6], and Graves disease (GD) [7]. Interestingly, patients with SSc and limited cutaneous disease have higher levels of sCD40LG in the plasma than those of the diffuse cutaneous disease [8]. Similarly, high levels of CD40 protein were observed in both the plasma [9] and the cell surface of skin fibroblasts [10] from SSc patients. In addition, previous studies reported the association of CD40 polymorphisms with susceptibility to a number of autoimmune diseases, such as GD [11], multiple sclerosis [12], RA [13, 14], Crohn disease [15], and with visual ischemic manifestations in individuals with biopsy-proven giant cell arteritis [16]. Nevertheless, in the case of SLE, contradictory data exist [1719]. In addition, mutations of CD40LG were observed in patients with the hyper-immunoglobulin M (IgM) syndrome [20], and genetic variations located at the 3UTR of the CD40LG gene were associated with two autoimmune diseases, SLE [21] and RA [22].

Taking into account these considerations, we aimed to investigate the potential association of CD40 and CD40LG genes polymorphism with SSc.

Materials and methods

Patients

In total, 2,670 SSc patients and 3,245 healthy individuals from four European populations were included in this study (Spain: cases, 1,103; controls, 1,610; Germany: cases, 554; controls, 437; The Netherlands: cases, 380; controls, 489; Italy: cases, 633; controls, 709). All the patients fulfilled the 1980 American College of Rheumatology (ACR) classification criteria for SSc [23]. Limited cutaneous disease (lcSSc) was defined as definite skin thickening confined to the distal extremities, whereas cases of diffuse cutaneous disease (dcSSc) required also the involvement of skin proximal to the knees and elbow [24, 25]. Measurement of main SSc-specific autoantibodies, anti-centromere antibodies (ACAs), and anti-topoisomerase I antibodies (ATAs), was performed by using standard methods. Pulmonary fibrosis data were investigated by using a computed tomography scan. The main features of all populations included in this study were previously reported [2628].

Patients and controls were included in the study after written informed consent, according to the declaration of Helsinki. The study was approved by local ethical committees from all the participating centers.

SNPs selection and genotyping

DNA from patients and controls was obtained by using standard methods. CD40 is located on chromosome 20q13.12. Three single-nucleotide polymorphisms (SNPs) of CD40 associated with other autoimmune diseases were selected. rs1883832 had been associated with GD [11]; whereas rs4810485, in linkage disequilibrium with rs1883832 (r 2 = 0.95), has been identified as a new risk factor for RA [13]. Furthermore, rs1535045 has been associated with subclinical atherosclerosis in diabetes families [29]. CD40LG is found on chromosome Xq26.3. Two genetic variants located in 5 UTR (rs3092952) and 3 UTR (rs3092920) of CD40LG (r 2 = 0.38) were selected. These SNPs are located in different haplotype blocks of CD40LG [17]. The variant rs3092920 is located near the 3 UTR microsatellite, which was previously associated with RA and SLE [21, 22]; whereas rs3092952 is a functional variant related to the levels of sCD40LG in plasma [30].

All SNPs were genotyped in the same center by using a TaqMan SNP genotyping assay in a 7900HT Real-Time polymerase chain reaction (PCR) system, by following the conditions recommended by the manufacturer (Applied Biosystems, Foster City, CA, USA). About 10% of the patients were genotyped twice to verify the genotyping consistency, showing 99% identical genotypes. The genotyping call-rate success was >95% for both cases and controls in all populations.

Statistical analysis

The Hardy-Weinberg equilibrium (HWE) was tested by means of the Fisher Exact test or χ2 when necessary. The case-control association study was performed by using a 2 × 2 contingency table with χ2 to obtain P values, odds ratios (ORs), and 95% confidence intervals (CIs). Combined OR was calculated according to a fixed-effects model (Mantel-Haenszel meta-analysis), and the heterogeneity of OR among all populations was calculated by using the Breslow-Day test. P values <0.05 were considered statistically significant. Statistical analyses were carried out with PLINK [31].

The estimation of the power of the study to detect an effect of a polymorphism in disease susceptibility was performed by using The CaTS Power Calculator software [32].

Results

CD40gene variants in SSc

Three CD40 polymorphisms were genotyped in four European populations. First, we analyzed the cohorts individually and then combined the samples in a pooled analysis. Table 1 describes allelic distribution of the three SNPs in the pooled analysis, and Additional file 1, Table S1-3 contains detailed data for each population. Both cases and controls were confirmed to be in HWE in all populations. No evidence of association between CD40 polymorphisms and susceptibility to SSc was observed in the pooled analysis (allelic P value rs1883832: P = 0.61; OR, 1.02; 95% CI, 0.94 to 1.11; rs4810485: P = 0.42; OR, 1.04; 95% CI, 0.95 to 1.13; rs1535045: P = 0.275; OR, 0.95; 95% CI, 0.88 to 1.04).
Table 1

Pooled analysis of CD40 polymorphisms

SNP

Change

Samples set

N

Minor allele,

no. (frequency)

Pvalue

OR [95% CI]

P-BD

rs1883832

T/C

Controls

3,138

1,427 (0.277)

   
  

SSc

2,605

1,424 (0.274)

0.610

1.02 [0.94-1.11]

0.904

  

lcSSc

1,758

987 (0.276)

0.246

1.06 [0.96-1.16]

0.496

  

dcSSc

860

451 (0.271)

0.683

0.97 [0.86-1.10]

0.446

  

ACA +

1,077

592 (0.261)

0.879

1.01 [0.90-1.13]

0.355

  

ATA +

716

416 (0.293)

0.108

1.11 [0.98-1.26]

0.946

  

Pulmonary fibrosis

702

394 (0.278)

0.433

1.05 [0.92-1.20]

0.802

rs4810485

G/T

Controls

3,122

1,652 (0.272)

   
  

SSc

2,560

1,388 (0.274)

0.420

1.04 [0.95-1.13]

0.935

  

lcSSc

1,736

967 (0.277)

0.155

1.07 [0.97-1.18]

0.481

  

dcSSc

839

436 (0.269)

0.817

0.99 [0.87-1.12]

0.600

  

ACA +

1,071

580 (0.257)

0.844

1.01 [0.90-1.13]

0.399

  

ATA +

699

402 (0.292)

0.080

1.12 [0.99-1.28]

0.890

  

Pulmonary fibrosis

682

375 (0.272)

0.483

1.05 [0.92-1.20]

0.605

rs1535045

T/C

Controls

3,147

1,595 (0.247)

   
  

SSc

2,585

1,272 (0.243)

0.275

0.95 [0.88-1.04]

0.560

  

lcSSc

1,749

860 (0.251)

0.375

0.96 [0.87-1.05]

0.334

  

dcSSc

850

411 (0.228)

0.275

0.93 [0.82-1.06]

0.746

  

ACA +

1,075

525 (0.241)

0.348

0.95 [0.84-1.06]

0.844

  

ATA +

707

369 (0.264)

0.669

1.03 [0.90-1.18]

0.640

  

Pulmonary fibrosis

696

340 (0.250)

0.396

0.94 [0.82-1.08]

0.028

Controls are used as reference for all comparisons. All P values have been calculated for the allelic model by using the Mantel-Haenszel test under fixed effect. P_BD, P value by the Breslow-Day method.

To investigate the possible influence of the CD40 polymorphisms with clinical features, we stratified the patients according to the main SSc manifestations. However, we did not observe evidence of association after comparing lcSSc and dcSSc with healthy subjects (see Table 1). Additionally, we compared the presence of SSc-specific autoantibodies in the patients with the healthy individuals, but no significant differences were observed (Table 1). Likewise, no significant association was observed when patients with pulmonary fibrosis were compared with healthy controls (Table 1).

CD40LGgene variants in SSc

Because CD40LG is located on the X-chromosome and shows the sexual bias of this disease, we performed the analysis separately for each gender. Table 2 shows the allelic frequencies in SSc females of the two CD40LG polymorphisms in the pooled analysis, and Additional file 1, Table S4-5 shows the frequencies for each population. Deviations from HWE were not observed. With the Mantel-Haenzel test, the genotype and allele frequencies were similar between SSc patients and healthy individuals (allelic P value rs3092952: P = 0.44; OR, 0.96; 95% CI, 0.85 to 1.07; rs3092920: P = 0.565; OR, 0.96; 95% CI, 0.83 to 1.11). No statistical differences were observed when SSc patients were stratified by common subtype of the disease, the presence of SSc-specific autoantibodies, and pulmonary fibrosis (Table 2).
Table 2

Pooled analysis of CD40LG polymorphisms in female SSc patients and controls

SNP

Change

Samples set

N

Minor allele,

no. (frequency)

Pvalue

OR [95% CI]

P_BD

rs3092952

G/A

Controls

1,795

655 (0.176)

   
  

SSc

2,082

735 (0.186)

0.440

0.96 [0.85-1.07]

0.18

  

lcSSc

1,446

515 (0.188)

0.592

0.97 [0.85-1.10]

0.12

  

dcSSc

635

219 (0.179)

0.307

0.92 [0.77-1.09]

0.73

  

ACA +

924

338 (0.195)

0.978

1.00 [0.87-1.16]

0.06

  

ATA +

533

184 (0.188)

0.375

0.92 [0.77-1.11]

0.50

  

Pulmonary fibrosis

542

198 (0.173)

0.735

0.97 [0.81-1.16]

0.41

rs3092920

T/G

Controls

1,789

376 (0.107)

   
  

SSc

2,104

426 (0.105)

0.565

0.96 [0.83-1.11]

0.89

  

lcSSc

1,456

310 (0.112)

0.929

1.01 [0.86-1.18]

0.97

  

dcSSc

647

116 (0.091)

0.120

0.84 [0.67-1.05]

0.36

  

ACA +

939

201 (0.111)

0.924

1.01 [0.84-1.21]

0.48

  

ATA +

541

106 (0.103)

0.434

0.91 [0.73-1.15]

0.86

  

Pulmonary fibrosis

549

124 (0.106)

0.657

1.05 [0.84-1.31]

0.55

Controls are used as reference for all comparisons. All P values have been calculated for the allelic model by using the Mantel-Haenszel test under fixed effect. P_BD, P value by the Breslow-Day method.

In addition, we analyzed these two polymorphisms in SSc male patients, but no evidence of association was found in the combined analyses (see Additional file 1, Table S6-7). However, these results should be interpreted with caution because the statistical power is insufficient to detect association because of low sample size.

Discussion

The important role of the CD40-CD40LG pathway in autoimmunity [4], together with the association of the CD40 gene with a number of autoimmune diseases, prompted us to investigate for the first time the contribution of CD40 and CD40LG genes in SSc.

Despite the previous findings [810], we observed no evidence of association of the CD40 or CD40LG gene variants analyzed with SSc. It was also the case when SSc patients were stratified by the SSc clinical subtypes, specific autoantibodies, or pulmonary fibrosis. We analyzed a large European population from four different countries to increase the robustness of the study. In this regard, our combined study had an estimated power of 92% to detect the relative risk, with OR of 1.15 obtained for RA susceptibility [13, 14], at the 5% significance level. Therefore, it seems unlikely that the absence of association found in our study would be due to Type II error.

In the present study, we analyzed the functional CD40-1C/T polymorphism (rs1883832). This genetic variant is located at -1 from the ATG, within a Kozak sequence, a stretch of nucleotides essential for translation that flanks the start codon in vertebrate genes [33]. The presence of a major allele (C) in this SNP is associated with the increase of the efficiency of CD40 translations [11]. Although quantitative differences between CD40 mRNA and proteins have been observed in SSc skin fibroblasts [10], the absence of association found for rs1883832 suggests that this variant might not affect the translation of CD40 mRNA. This process may be upregulated in these abnormal skin fibroblasts, or other genes of the CD40 signaling pathway may influence the CD40 expression. However, functional studies in this way should be constructed before excluding an association between this variant and the CD40 expression in SSc.

Although CD40 might be a common susceptibility locus for some autoimmune diseases [1116], our results do not suggest an important role of CD40 in the susceptibility to SSc. Several genes have been recently disclosed to play a function in the susceptibility to autoimmune diseases, suggesting that these diseases share a genetic background [34]. However, these loci may not be universal genetic factors for autoimmune disorders; therefore, the autoimmunity might result from specific and multiple pleiotropic effects [19]. Also, other genes are unique for each disease, reflecting a specific etiology [19, 34]. Additional studies are required for the identification of specific and shared genetic pathways that contribute to a better understanding of the pathogenesis of the autoimmune diseases.

The role of the CD40LG in the susceptibility to autoimmune diseases has not been investigated as broadly as that of CD40, mainly because this gene is located on the × chromosome. The different prevalences of these diseases in both genders can suggest that genes located on the × chromosome could be susceptibility factors in autoimmune diseases; however, few studies analyzed polymorphisms on this chromosome. Mutations on this gene are associated with X-linked hyper-IgM syndrome, a familial genetic disorder characterized by an increase of IgM level and a decrease of IgG and IgA [20], but in SLE, no evidence of association has been found [17]. Similarly, our results show that the CD40LG gene may not be SSc susceptibility loci.

Conclusions

Our results do not suggest an important role of CD40 and CD40LG genes in the susceptibility to SSc. Additional studies are required to draw firm conclusions about the exact role of the CD40 and CD40LG genes in SSc susceptibility because other variants might be involved in SSc. Future studies involving other genes of the CD40-CD40LG pathway should be conducted to elucidate fully the contribution of this pathway in the pathogenesis of SSc.

Abbreviations

ACA: 

anti-centromere antibody

ATA: 

anti-topoisomerase I antibody

CI: 

confidence interval

dcSSc: 

diffuse cutaneous subtype

GD: 

Graves disease

GWAS: 

genome-wide association study

HWE: 

Hardy-Weinberg equilibrium

lcSSc: 

limited cutaneous subtype

OR: 

odds ratio

P-BD P

value by Breslow-Day method

RA: 

rheumatoid arthritis

SLE: 

systemic lupus erythematosus

SNP: 

single-nucleotide polymorphism

SSc: 

systemic sclerosis.

Declarations

Acknowledgements

We thank Sofia Vargas, Sonia Garcia, and Gema Robledo for their excellent technical assistance, and all the patients and healthy controls for kindly accepting their essential collaboration. We thank Banco Nacional de ADN (University of Salamanca, Spain) for supplying part of the control material. We also thank EUSTAR (The EULAR Scleroderma Trials and Research Group) and the German Network of Systemic Sclerosis for the facilitation of this project.

This work was supported by the following grants. JM was funded by SAF2009-11110 from the Spanish Ministry of Science, by CTS-4977 and PI-0590-2010 from Junta de Andalucía, and by RETICS Program, RD08/0075 (RIER) from Instituto de Salud Carlos III (ISCIII), within the VI PN de I+D+i 2008-2011 (FEDER). T.R.D.J.R. was funded by the VIDI laureate from the Dutch Association of Research (NWO) and Dutch Arthritis Foundation (National Reumafonds). JM and TRDJR were sponsored by the Orphan Disease Program grant from the European League Against Rheumatism (EULAR). TW was awarded grants by DFG WI 1031/6.1 and DFG KFO 250 TP03. MT was supported by Spanish Ministry of Science through the program Juan de la Cierva (JCI-2010-08227).

Spanish Scleroderma Group:

Norberto Ortego-Centeno, José Luis Callejas, and Raquel Ríos, Systemic Autoimmune Diseases Unit, Department of Internal Medicine, Hospital Clínico Universitario San Cecilio, Granada; Nuria Navarrete and Antonio Garcia, Department of Internal Medicine, Hospital Virgen de las Nieves, Granada; Antonio Fernández-Nebro, Department of Rheumatology, Hospital Carlos Haya, Málaga; María F. González-Escribano, Department of Immunology, Hospital Virgen del Rocío, Sevilla; Julio Sánchez-Román and Mª Jesús Castillo, Department of Internal Medicine, Hospital Virgen del Rocío, Sevilla; Mª Ángeles Aguirre and Inmaculada Gómez-Gracia, Department of Rheumatology, Hospital Reina Sofía, Córdoba; Benjamín Fernández-Gutiérrez and Luis Rodríguez-Rodríguez, Department of Rheumatology, Hospital Clínico San Carlos, Madrid; Esther Vicente, Department of Rheumatology, Hospital La Princesa, Madrid; Mónica Fernández Castro and José Luis Andreu, Department of Rheumatology, Hospital Puerta del Hierro, Madrid; Paloma García de la Peña, Department of Rheumatology, Hospital Universitario Madrid Norte Sanchinarro, Madrid; Francisco Javier López-Longo and Lina Martínez-Estupiñán, Department of Rheumatology, Hospital General Universitario Gregorio Marañón, Madrid; Anna Pros, Department of Rheumatology, Hospital Del Mar, 08003 Barcelona; Vicente Fonollosa, Department of Internal Medicine, Hospital Valle de Hebrón, Barcelona; Carlos Tolosa, Department of Internal Medicine, Hospital Parc Tauli, Sabadell; Mónica Rodríguez Carballeira, Department of Internal Medicine, Hospital Universitari Mútua Terrasa, Barcelona; Ivan Castellví, Unidad de Reumatología, Department of Internal Medicine, Hospital de la Santa Creu i Sant Pau, Barcelona; Francisco Javier Narváez, Department of Rheumatology, Hospital Universitari de Bellvitge, Barcelona; Francisco Javier Blanco-García, Natividad Oreiro, and María Ángeles Robles, Department of Rheumatology, INIBIC-Hospital Universitario A Coruña, A Coruña; María Victoria Egurbide, Department of Internal Medicine, Hospital de Cruces, Vizcaya; Luis Sáez-Comet, Systemic Autoimmune Diseases Unit, Department of Internal Medicine, Hospital Universitario Miguel Servet, Zaragoza; Ricardo Blanco, Department of Rheumatology, Hospital Universitario Marqués de Valdecilla, Santander; Bernardino Díaz and Luis Trapiella, Department of Internal Medicine, Hospital Central de Asturias, Oviedo; Federico Díaz and Vanesa Hernández, Department of Rheumatology, Hospital Universitario de Canarias, Tenerife; Emma Beltrán, Department of Rheumatology, Hospital del Doctor Peset Aleixandre, Valencia; and José Andrés Román-Ivorra, Department of Rheumatology, Hospital Universitari i Politecnic La Fe, Valencia.

Authors’ Affiliations

(1)
Instituto de Parasitología y Biomedicina López-Neyra, IPBLN-CSIC
(2)
Department of Internal Medicine, Hospital Valle de Hebron
(3)
Department of Rheumatology, Radboud University Nijmegen Medical Centre
(4)
Department of Rheumatology, Hospital 12 de Octubre
(5)
Department of Internal Medicine, Hospital Carlos Haya
(6)
Department of Rheumatology, Hospital Virgen de la Victoria
(7)
Department of Rheumatology, Hospital Universitario de Canarias
(8)
Department of Internal Medicine, Hospital Central de Asturias
(9)
Department of Autoimmune Diseases, Hospital Clinic
(10)
See Acknowledgements
(11)
Referral Center for Systemic Autoimmune Diseases, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Via Francesco Sforza
(12)
Rheumatology Unit and Chair, Spedali Civili, Università degli Studi
(13)
Department of Medicine, Policlinico GB Rossi, Università degli studi di Verona
(14)
Department of Rheumatology and Clinical Immunology, Charité University Hospital and German Rheumatism Research Centre, a Leibniz Institute
(15)
Clinic for Immunology and Rheumatology Medical School
(16)
Department of Dermatology, University of Cologne
(17)
Department of Dermatology, Allergology, and Venereology, Ruhr University of Bochum
(18)
Department of Internal Medicine 3, Institute for Clinical Immunology, University of Erlangen-Nuremberg
(19)
Section Complex Genetics, Department of Medical Genetics, University Medical Center Utrecht
(20)
Department of Rheumatology, VU University Medical Center
(21)
Department of Rheumatology, Leiden University Medical Center
(22)
Department of Rheumatology, Hospital Universitario Marques de Valdecilla, IFIMAV
(23)
Department of Rheumatology and Clinical Immunology, University Utrecht Medical Center

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