Staphylococcus aureus and Wegener's granulomatosis
© BioMed Central Ltd 2002
Received: 17 September 2001
Accepted: 18 October 2001
Published: 26 October 2001
Wegener's granulomatosis (WG) is a form of systemic vasculitis. It is characterized by granulomatous inflammation in the upper and lower airways, vasculitis and necrotizing glomerulonephritis, and is strongly associated with antineutrophil cytoplasmic antibodies against proteinase 3. Since the etiology of the disease is not clear, treatment, consisting of corticosteroids and immunosuppressives, is nonspecific and associated with severe side effects. Pinpointing the trigger(s) of the disease would highly improve treatment. Clinical evidence shows that an infectious agent, the bacterium Staphylococcus aureus, is a risk factor for disease relapse, suggesting its involvement in the pathogenesis of WG. Here we review both clinical and experimental data that either indicate or support a role for S. aureus in WG.
Keywordsautoimmunity co-trimoxazole Staphylococcus aureus superantigens Wegener's granulomatosis
Wegener's granulomatosis (WG) is a form of systemic vasculitis that predominantly affects small- and medium-sized blood vessels. Frequently commencing with general, aspecific symptoms, the disease is frequently hallmarked by the presence of autoantibodies (anti neutrophil cytoplasmic antibodies [ANCA]) [1,2] directed predominantly against the myeloid enzyme proteinase 3 (in 80% of WG patients) and, in a minority of cases, against myeloperoxidase [3,4]. Manifestations of granulomatous inflammation in the upper and lower airways, vasculitis and necrotizing glomerulonephritis characterize the classical clinical picture of WG .
In WG, respiratory tract infections frequently precede or accompany initial symptoms [6,7]. Moreover, treatment of WG limited to the airways with the antibiotic co-trimoxazole often leads to achievement of stable remission  and prophylactic treatment with this drug reduced the incidence of disease relapses . Although the beneficial effect of co-trimoxazole may also be explained by its immunosuppressive effect  and although a bacterial agent was originally not pinpointed [9,11], these findings were taken as indications that bacterial infections may be essentially implicated in the pathogenesis of WG. In search of a specific pathogenic bacterial agent we have shown that chronic nasal carriage of Staphylococcus aureus is approximately three times higher in WG patients than in healthy individuals , which may, at least in part, be due to hospitalization and/or immunosuppressive treatment. In contrast to healthy individuals who are frequently exposed to S. aureus (e.g. hospital personnel), but for whom carriage of this bacterium remains inconsequential, in WG patients S. aureus constitutes a risk factor for disease exacerbation . This may possibly be due to a dysregulated immune system and frequent nasal lesions present in these patients. Thus, these data ask for exploration of the potential mechanisms mediating staphylococcal pathogenicity. Recently obtained data supporting various pathogenic mechanisms related to S. aureus will be reviewed below.
Staphylococcal superantigens and WG
In vitro and in animal models S. aureus acts as a potent immunostimulator, effecting polyclonal proliferation of T and B cells, secretion of immunoglobulins, and cytokine production. The main influence of S. aureus on immunocompetent cells is exerted by its exotoxins. Three classes of staphylococcal exotoxins have been described so far: the staphylococcal enterotoxins, exfoliative toxins, and the toxic-shock-syndrome-toxin-1 (TSST-1) . Based on their exceptionally strong capacity to stimulate T cells in a non antigen specific way, exotoxins were termed 'super-antigens' (SAg) . Moreover, staphylococcal protein A (SpA), a common component of the staphylococcal cell wall, has been recognized as a B-cell SAg .
T-cell SAg can simultaneously bind to MHC class II molecules outside the peptide-binding groove and to conserved regions of specific families of T-cell receptor V-beta chains (TCR Vβ) , independently of ligand specificity of T cells. As a consequence, virtually all T cells expressing a SAg-reactive TCR Vβ chain are stimulated to proliferate.
In the absence of more formal proof, exaggerated proliferation of SAg-specific T-cell subsets has been regarded as an indicator of SAg involvement in disease. Indeed, the presence of abnormal expansions of Vβ2-expressing T cells during the active phase of Kawasaki disease (a form of large- to medium-size vessel vasculitis) together with the detection of TSST-1-producing S. aureus in this disease, strongly suggested a causal link between immune dysbalance, disease activity and SAg [15,16,17,18]. In WG, several groups have reported expansions of various T-cell subsets but they found no obvious preferential skewing of any particular Vβ subset [19,20]. Moreover, we were unable to demonstrate a link between staphylococcal SAg and peripheral blood T-cell expansions in patients with WG, since expansions of peripheral blood T-cell subsets were not associated with the simultaneous presence of SAg-positive S. aureus strains (Popa et al. manuscript in preparation).
These findings, however, do not exclude a pathophysiologic role for staphylococcal SAg in WG. In a study including 709 S. aureus strains (isolated from nasal cultures from 63 WG patients followed over a time period of 6 years) we screened for genes encoding the staphylococcal enterotoxin SAg SEA-E, exfoliative toxin A and TSST-1. We assessed the risk for development of a disease relapse within three months from the appearance of one of these SAgs. We again confirmed the association of the presence of S. aureus with a risk for relapse of WG. In addition, we found that the risk for relapse was modulated according to the presence and type of SAg, with tsst-1 being associated with a higher risk for relapse (relative risk 14.5, 95% confidence interval 2.4–85.4). In WG, a pathogenic role for S. aureus in general, and the SAg TSST-1 in particular, thus cannot be excluded and the mechanisms of pathogenicity should be investigated further. An important goal for investigation will be the demonstration of SAg in the blood stream and lymphoid organs of WG patients.
The B-cell SAg, SpA, is a component of most clinical strains of S. aureus. The superantigenic activity of SpA is induced after binding to membrane immunoglobulin heavy chains encoded by VH3 family genes , which constitute 15–50% of total human IgM, IgG and IgA, and effects mitogenicity and induction of polyclonal antibody responses.
In WG, the possible implication of SpA can be conjectured from the amino acid sequence of WG-associated ANCA. Thus, the presence of ANCA with a VH3-encoded heavy chain would indicate potential binding capacity of SpA. Based on an anecdotic report of VH3-encoded ANCA  it is conceivable that, in the presence of SpA, ANCA-producing B cells could be induced to proliferate, resulting in an amplification of the pool of autoantibodies. The fact that carriage of S. aureus in patients with WG was associated with increased ANCA positivity during follow-up  supports this potential mechanism.
Staphylococcal acid phosphatase
In patients with S. aureus-associated glomerulonephritis, high levels of circulating immune complexes are present which decrease during antibiotic treatment [22,23]. These findings raise the possibility that specific staphylococcal molecules may be nephritogenic. One of these molecules is the cationic acid phosphatase, SAcP , which has a high affinity for the glomerular basement membrane and can induce glomerulonephritis when perfused in the kidney of naïve rats  or rats immunized with SAcP . Based on these data, we hypothesized that, in WG, SAcP may act as a planted antigen by binding to glomerular basement membrane and vascular endothelium, thus initiating glomerulonephritis and vasculitis. We found that SAcP can bind to human umbilical vein endothelial cells and glomerular endothelial cells in vitro  in a charge-dependent manner, since binding can be blocked by the negatively charged heparin. Moreover, sera of WG patients were able to bind SAcP bound to endothelial cells in vitro . Together with our finding that antibodies to SAcP are present in WG patients who are S. aureus carriers, these data suggest that, in vivo, the presence of SAcP in conjunction with anti-SAcP antibodies may be nephritogenic and vasculitogenic in WG patients. Moreover, in the presence of SAcP–anti-SAcP immune complexes in the kidney, ANCA may be able to aggravate glomerulonephritis, as suggested by our studies on the effect of myeloperoxidase-specific autoantibodies on anti-glomerular-basement-membrane-mediated glomerular injury in the rat .
To date, despite clinical evidence suggesting that S. aureus may be implicated in the pathophysiology of WG, laboratory investigation of the possible mechanisms by which S. aureus is involved in WG is still scarce. Besides staphylococcal SAg and SAcP, which have received some attention, other staphylococcal molecules, such as hemolysins, teichoic acid, proteoglycans and DNA-containing CpG motifs, to name only a few, are currently known as immunomodulators and certainly deserve further investigation in the context of vasculitis. Moreover, there are ample in vitro and in vivo data available on the effect of S. aureus on immunocompetent cells, such as B and T cells, monocytes and neutrophillic granulocytes, but also on other cell types, such as endothelial and epithelial cells. Keeping in mind that in WG, immune dysbalance can eventually lead to vascular damage, S. aureus as a trigger and mediator of various pathophysiologic mechanisms is a very attractive target for investigation.
anti neutrophil cytoplasmic antibody
staphylococcal acid phosphatase
staphylococcal protein A
- TCR Vβ:
T-cell receptor V-beta chains
- van der Woude FJ, Rasmussen N, Lobatto S, Wiik A, Permin H, van Es LA, van der Giessen M, van der Hem GK, The TH: Autoantibodies to neutrophils and monocytes: a new tool for diagnosis and a marker of disease activity in Wegener's gran-ulomatosis. Lancet. 1985, i: 425-429. 10.1016/S0140-6736(85)91147-X.View ArticleGoogle Scholar
- Cohen Tervaert JW, van der Woude FJ, Fauci AS, Ambrus JL, Velosa J, Keane WF, Meijer S, van der Giessen M, The TH, van der Hem GK, Kallenberg CGM: Association between active Wegener's granulomatosis and anticytoplasmic antibodies. Arch Int Med. 1989, 149: 2461-2465. 10.1001/archinte.149.11.2461.View ArticleGoogle Scholar
- Falk RJ, Jennette JC: Anti-neutrophil cytoplasmic autoantibod-ies with specificity for myeloperoxidase in patients with systemic vasculitis and idiopathic necrotizing and crescentic glomerulonephritis. N Engl J Med. 1988, 318: 1651-1657.PubMedView ArticleGoogle Scholar
- Kallenberg CGM, Brouwer E, Weening JJ, Cohen Tervaert JW: Anti-neutrophil cytoplasmic antibodies: Current diagnostic and pathophysiological potential. Kidney Int. 1994, 46: 1-15.PubMedView ArticleGoogle Scholar
- Jennette JC, Falk RJ, Andrassy K, Bacon PA, Churg J, Gross WL, Hagen EC, Hoffman GS, Hunder GG, Kallenberg CG, McCluskey RT, Sinico AR, Rees AJ, van Es LA, Waldherr R, Wiika A: Nomenclature of systemic vasculitides. Proposal of an international consensus conference. Arthritis Rheum. 1994, 37: 187-192.PubMedView ArticleGoogle Scholar
- Fauci AS, Haynes BF, Katz P, Wolff SM: Wegener's granulomatosis: prospective clinical and therapeutic experience with 85 patients for 21 years. Ann Intern Med. 1983, 98: 76-85.PubMedView ArticleGoogle Scholar
- Pinching AJ, Rees AJ, Pussell BA, Lockwood CM, Mitchison RS, Peters DK: Relapses in Wegener's granulomatosis: the role of infection. Br Med J. 1980, 281: 836-838.PubMedPubMed CentralView ArticleGoogle Scholar
- DeRemee RA, McDonald TJ, Weiland LH: Wegener's granulomatosis:observations on treatment with antimicrobial agents. Mayo Clin Proc. 1985, 60: 27-32.PubMedView ArticleGoogle Scholar
- Stegeman CA, Cohen Tervaert JW, de Jong PE, Kallenberg CGM: Trimethoprim-sulfamethoxazole for the prevention of relapses of Wegener's granulomatosis. New Engl J Med. 1996, 335: 16-20. 10.1056/NEJM199607043350103.PubMedView ArticleGoogle Scholar
- Roberts DE, Curd JG: Sulfonamides as anti-inflammatory agents in the treatment of Wegener's granulomatosis. Arthritis Rheum. 1990, 33: 1590-1593.PubMedView ArticleGoogle Scholar
- Stegeman CA, Cohen Tervaert JW, Sluiter WJ, Manson WL, de Jong PE, Kallenberg CGM: Association of chronic nasal carriage of S. aureus and higher relapse rates in Wegener's granulomatosis. Ann Int Med. 1994, 120: 12-17.PubMedView ArticleGoogle Scholar
- McCormick JK, Yarwood JM, Schlievert PM: Toxic shock syndrome and bacterial superantigens: An update. Annu Rev Microbiol. 2001, 55: 77-104. 10.1146/annurev.micro.55.1.77.PubMedView ArticleGoogle Scholar
- Marrack P, Kappler J: The staphylococcal enterotoxins and their relatives. Science. 1990, 248: 705-711.PubMedView ArticleGoogle Scholar
- Sasso EH, Silverman GJ, Mannik M: Human IgM molecules that bind staphylococcal protein A contain VHIII heavy chains. J Immunol. 1989, 142: 2778-2783.PubMedGoogle Scholar
- Abe J, Kotzin BL, Jujo K, Melish ME, Glode MP, Kohsaka T, Leung DY: Selective expansion of T-cell receptor variable regions Vβ2 and Vβ8 in Kawasaki disease. Proc Natl Acad Sci USA. 1992, 89: 4966-4970.Google Scholar
- Abe J, Kotzin BL, Messner C, Melish ME, Takahashi M, Fulton D, Romagne F, Malissen B, Leung DY: Characterization of T cell repertoire changes in acute Kawasaki disease. J Exp Med. 1993, 177: 791-796.PubMedView ArticleGoogle Scholar
- Leung DYM, Meissner HC, Fulton DR, Murray DL, Kotzin BL, Schlievert PM: Toxic shock syndrome toxin-secreting Staphylococcus aureus in Kawasaki syndrome. Lancet. 1993, 342: 1385-1388. 10.1016/0140-6736(93)92752-F.PubMedView ArticleGoogle Scholar
- Leung DYM, Sullivan KE, Brown-Whitehorn TF, Fehringer AP, Allen S, Finkel TH, Washington RL, Makida R, Schlievert PM: Association of toxic shock syndrome-secreting and exfoliative toxin-secreting Staphylococcus aureus with Kawasaki syndrome complicated by coronary artery disease. Pediatr Res. 1997, 42: 268-272.PubMedView ArticleGoogle Scholar
- Giscombe R, Grunewald J, Nityanand S, Lefvert AK: T cell receptor (TCR) V gene usage in patients with systemic necrotizing vasculitis. Clin Exp Immunol. 1995, 101: 213-219.PubMedPubMed CentralView ArticleGoogle Scholar
- Simpson IJ, Skinner MA, Geursen A, Peake JS, Abbott WG, Fraser JD, Lockwood CM, Tan PL: Peripheral blood T lymphocytes in systemic vasculitis: increased T cell receptor Vβ2 gene usage in microscopic polyarteritis. Clin Exp Immunol. 1995, 101: 220-226.PubMedPubMed CentralView ArticleGoogle Scholar
- Sibilia J, Benlagha K, Vanhille P, Ronco P, Brouet JC, Mariette X: Structural analysis of human antibodies to proteinase 3 from patients with Wegener granulomatosis. J Immunol. 1997, 159: 712-719.PubMedGoogle Scholar
- Pertschuk L, Woda B, Vuletin J, Brigati DJ, Soriano CB, Nicastri AD: Glomerulonephritis due to Staphylococcus aureus antigen. Am J Clin Pathol. 1976, 65: 301-307.PubMedGoogle Scholar
- Sato M, Nakazoro H, Ofuji T: The pathogenic role of Staphylococcus aureus in primary human glomerulonephritis. Clin Nephrol. 1979, 11: 190-195.PubMedGoogle Scholar
- Yousif Y, Schlitz E, Okada K, Batsford S, Vogt A: Staphylococcal neutral phosphatase. A highly cationic molecule with binding properties for immunoglobulin. APMIS. 1994, 102: 891-900.PubMedView ArticleGoogle Scholar
- Yousif Y, Okada K, Batsford S, Vogt A: Induction of glomerulonephritis in rats with staphylococcal phosphatase: new aspects in post-infectious ICGN. Kidney Int. 1996, 50: 290-297.PubMedView ArticleGoogle Scholar
- Brons RH, Klok P, van Dijk N, Kallenberg CGM, Tiebosch A, Cohen Tervaert JW: Staphylococcal acid phosphatase induces severe crescentic glomerulonephritis in immunized Brown-Norway rats: relevance for Wegener's granulomatosis? [Abstract]. Clin Exp Immunol. 2000, 120: 44-Google Scholar
- Brons RH, Bakker HI, van Wijk RT, van Dijk NW, Muller Kobold AC, Limburg PC, Manson WL, Kallenberg CG, Cohen Tervaert JW: Staphyloccal acid phosphatase binds to endothelial cells via charge interaction: a pathogenic role in Wegener's granulomatosis?. Clin Exp Immunol. 2000, 119: 566-573. 10.1046/j.1365-2249.2000.01172.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Heeringa P, Brouwer E, Klok PA, Huitema MG, van den Born J, Weening JJ, Kallenberg CGM: Autoantibodies to myeloperoxidase aggravate mild anti-glomerular-basement-membrane-mediated glomerular injury in the rat. Am J Pathol. 1996, 149: 1695-1706.PubMedPubMed CentralGoogle Scholar