Citrullination of fibronectin modulates synovial fibroblast behavior
© Shelef et al.; licensee BioMed Central Ltd. 2012
Received: 3 July 2012
Accepted: 30 October 2012
Published: 5 November 2012
Rheumatoid arthritis is an autoimmune arthritis characterized by joint destruction. Anti-citrullinated protein antibodies are pathologic in rheumatoid arthritis, but the role of the citrullinated proteins themselves is much less clear. Citrullination is the conversion of the arginine residues of a protein to citrulline. In the inflamed rheumatoid joint there is increased protein citrullination. Several proteins are citrullinated in rheumatoid arthritis, including collagen type II, fibrinogen, and fibronectin. Fibronectin is thought to mediate the adhesion of joint-invading synovial fibroblasts to the rheumatoid cartilage in addition to regulating other synovial fibroblast functions. However, the effect of citrullinated fibronectin on synovial fibroblasts is unknown.
To investigate the effect of citrullinated fibronectin on synovial fibroblast behavior, we cultured normal murine, arthritic murine, and human rheumatoid synovial fibroblasts. We then compared several synovial fibroblast functions in the presence of fibronectin versus citrullinated fibronectin. We assessed adhesion with time-lapse microscopy, migration with transwell assays, focal adhesion kinase and paxillin phosphorylation by western blot, and focal matrix degradation by fluorescent gelatin degradation.
Normal synovial fibroblasts have impaired adhesion, spreading, migration, and integrin-mediated phosphorylation of focal adhesion kinase and paxillin on citrullinated fibronectin. Murine arthritic and human rheumatoid synovial fibroblasts also have impaired adhesion and spreading on citrullinated fibronectin, but focal matrix degradation is unaffected by citrullinated fibronectin.
Citrullination of fibronectin alters synovial fibroblast behavior and may affect how these cells adhere to and invade the joint and travel through the bloodstream. This work suggests an important role for the interaction of synovial fibroblasts with citrullinated matrix in the pathophysiology of rheumatoid arthritis.
Rheumatoid arthritis is a chronic, debilitating arthritis characterized by painful joint inflammation and destructive erosions. Rheumatoid arthritis has long been known to be an inflammatory arthritis, but only recently has it been shown to be a true autoimmune disease with an immune response generated against self-antigens. The key to this finding was the identification of anti-citrullinated protein antibodies (ACPAs), which contribute to arthritis by forming immune complexes that are deposited in the joint  and by activating complement . ACPAs are specific for rheumatoid arthritis and predict severe, erosive arthritis .
Despite the rapidly increasing volume of information about ACPAs, the role of the citrullinated proteins themselves is less clear. Citrullination is the conversion of a protein's arginine residues to citrulline, resulting in a loss of charge and often abnormal protein conformation and function. Citrullination is catalyzed by a family of enzymes called peptidyl arginine deiminases (PADs). Protein citrullination in the rheumatoid joint is increased  and PAD4 and PAD2 are upregulated in rheumatoid synovium [5, 6] and synovial fluid . Inflammation appears to play a role in the level of citrullination since local administration of glucocorticoids reduces citrullination in the rheumatoid joint . Despite the specificity of ACPAs to rheumatoid arthritis, however, citrullination is a more generalized phenomenon - with increased citrullination seen in the synovial fluid of inflamed joints affected by spondyloarthropathy  as well as in inflamed muscle in myositis and  myelin basic protein in multiple sclerosis .
The role of protein citrullination in rheumatoid arthritis is enigmatic, although most evidence supports a pathologic role. Citrullinated fibrinogen  and citrullinated collagen type II  are more immunogenic and arthritogenic in mouse models of arthritis, and citrullinated fibrinogen activates macrophages more than unmodified fibrinogen . Further, treatment with Cl-amidine, a pan-PAD inhibitor, improves collagen-induced arthritis . In contrast, citrullinated CXCL10, CXCL11 , IL-8 , and CXCL12  lose inflammatory function, but these proteins have not been shown to be citrullinated in rheumatoid arthritis.
Some proteins that have been shown to be citrullinated in rheumatoid arthritis include type II collagen, vimentin, fibrinogen, and fibronectin [17, 18]. Fibronectin is interesting because it modulates numerous cellular behaviors including migration, adhesion, invasion, and survival. More specific to rheumatoid arthritis, fibronectin is deposited on the surface of articular cartilage in the rheumatoid joint  and increases the ability of synovial fibroblasts to adhere to cartilage .
Synovial fibroblasts are cells that normally line the joint. These fibroblasts play a significant role in rheumatoid arthritis by increasing in number as part of a pannus and by degrading cartilage and bone using matrix metalloproteases and invasive structures called invadopodia . Synovial fibroblasts also can migrate in the bloodstream to invade distant cartilage in mouse models , which may explain how multiple joints are involved in rheumatoid arthritis.
Fibronectin plays an important role in synovial fibroblast behavior. Synovial fibroblasts express the integrins α5β1 and αvβ3, which are important for fibronectin binding , and fibronectin is present throughout the pannus . Further, fibronectin mediates synovial fibroblast adhesion to cartilage, stabilizes invadopodia , and modulates matrix metalloprotease production .
Despite the importance of fibronectin in synovial fibroblast and arthritis pathology, almost nothing is known about the function of citrullinated fibronectin. We know only that there is increased affinity of citrullinated fibronectin for vascular endothelial growth factor but decreased affinity for β1 integrin and decreased ability of citrullinated fibronectin to cause apoptosis of HL-60 cells .
Given the presence of citrullinated fibronectin in rheumatoid arthritis, the paucity of information about citrullinated fibronectin, and the importance of the interactions of fibronectin and synovial fibroblasts in rheumatoid arthritis, we decided to investigate how citrullinated fibronectin modulates synovial fibroblast behavior. We report in this paper that citrullinated fibronectin leads to impaired adhesion, spreading, migration, and integrin-mediated signaling of synovial fibroblasts, yet focal degradation is unchanged.
Materials and methods
De-identified synovial fluid samples from patients given a diagnosis of rheumatoid arthritis by a rheumatologist were provided by physicians not associated with this research. Joint aspiration was part of routine care for the patients. This research does not meet the definition of human subjects research or require informed consent as confirmed by the University of Wisconsin Institutional Review Board.
TNF transgenic mice (line 3647)  were generously provided by Dr Edward Schwarz and permission for their use was generously provided by Dr George Kollias at the Alexander Fleming Biomedical Sciences Research Centre. TNF transgenic and C57BL/6 mice were cared for and euthanized in a manner approved by the University of Wisconsin Animal Care and Use Committee.
Synovial fibroblast cultures
Synovial fibroblast cultures were prepared from mouse ankle joints using standard methods and from human synovial fluid according to published protocols . Briefly, mice were euthanized and ankle joints were removed, minced, and incubated for 2 to 3 hours in a collagenase solution at 37°C to release cells for plating in a media of DMEM with 10% fetal bovine serum (FBS), L-glutamine, nonessential amino acids, essential amino acids, penicillin, streptomycin, and β-mercaptoethanol. Human synovial fluid was centrifuged for 10 to 20 minutes and the cell pellet resuspended in the above media. Sometimes trypsin was used to help disrupt cell clumps in synovial fluid. All synovial fibroblasts were used between passages 5 and 9. Before use, mouse synovial fibroblasts were confirmed to express vascular cell adhesion molecule-1 and not F4/80 or CD45 and human synovial fibroblasts were confirmed to express CD90 and not CD68 (data not shown). Cells were serum starved overnight before all experiments in media identical to above, but with only 0.1% FBS.
Citrullination in vitro
Fibronectin was purified from human plasma by affinity chromatography as described previously . This fibronectin was citrullinated as previously described by incubating 500 mg overnight at 37°C in a buffer of 100 mM Tris, pH 7.4, and 5 mM CaCl2 with 5 units PAD isolated from rabbit skeletal muscle (Sigma-Aldrich St. Louis, MO, USA) in a total of 1 ml . As controls, fibronectin was incubated in buffer alone or in buffer with PAD that had been heat-inactivated at 95°C for 20 minutes. Heat-inactivation of citrullinating capability was confirmed by a colorimetric assay using published protocols . Fibronectin samples were subjected to SDS-PAGE followed by staining with Coomassie Blue using standard methods to evaluate mobility shift.
Nontissue culture-treated 35 mm petri dishes (BD Biosciences San Jose, CA USA) were coated by incubation at 37°C for 1.5 hours with 10 μg/ml fibronectin, citrullinated fibronectin, or fibronectin that had been incubated with heat-inactivated PAD. Dishes were washed with PBS, blocked with 2% BSA, and washed again with PBS. Equivalent coating was assessed by coating and washing plastic dishes, and then incubating with BCA reagent (Thermo Scientific Rockford, IL USA) according to the manufacturer's instructions using a protein standard.
Cell spreading and attachment assay
Synovial fibroblasts were incubated on plates coated with BSA, fibronectin, citrullinated fibronectin, or fibronectin exposed to heat-inactivated PAD. A 10× field that contained an average of 30 cells was chosen and time-lapse microscopy was used to image the field every minute. Every 5 minutes the percentage of cells that were no longer floating and had begun to spread and attach was assessed. Cells that were touching other cells or were not completely in the field of view were excluded.
Murine synovial fibroblasts (2 × 104) in 0.1% FBS media were placed in the upper chamber of a transwell. The lower chamber contained 0.1% FBS media with no additive, 10 μg/ml fibronectin, 10 μg/ml citrullinated fibronectin, or 10 μg/ml fibronectin that had been incubated with heat-inactivated PAD. After 6 hours, nonmigrated cells were removed with a cotton swab and the membranes were fixed in methanol and stained with H & E.
For western blot of fibronectin, the fibronectin samples were run by SDS-PAGE under nonreducing conditions, transferred to nitrocellulose and blotted with the anti-fibronectin antibodies 4D1 and 5C3 . For western blots of cell lysates, adherent cells were scraped into lysis buffer (5 mM Tris, pH 7.4; 500 mM NaCl; 0.1% SDS; 0.5% sodium deoxycholate; 0.5 mM MgCl2) on ice containing 2 μg/ml aprotinin, 1 μg/ml leupeptin, 1 μg/ml pepstatin, 0.2 mM phenylmethylsulfonyl fluoride and 1 mM sodium vanadate. For the BSA control, floating cells were used because the cells do not adhere. Equivalent amounts of cell lysates were subjected to SDS-PAGE and transferred to nitrocellulose for western blotting using anti-focal adhesion kinase (anti-FAK, clone 77; BD Biosciences), anti-paxillin (clone 349; BD Biosciences), anti-phospho-Tyr-397 FAK (polyclonal; Life Technologies, Grand Island, NY, USA), and anti-phospho-Tyr-118 paxillin (polyclonal; Life Technologies) followed by imaging with an Odyssey infrared imaging system (LI-COR Biosciences, Lincoln, Nebraska USA).
Gelatin degradation assay
Gelatin coverslips were prepared as published previously . Briefly, coverslips were treated with 50 μg/ml poly-L-lysine, then 0.5% glutaraldehyde, then 0.2% Oregon green gelatin, and then 5 mg/ml NaBH4 with PBS washing steps between each treatment. Coverslips were then coated with 10 μg/ml fibronectin or citrullinated fibronectin as described above. About 10,000 synovial fibroblasts from TNF transgenic mice were plated for 4 hours on the coverslips before fixation with 3% formaldehyde, quenching with glycine, permeabilization with 0.2% Triton-X 100, blocking with goat serum and incubating with mouse anti-cortactin (clone 4F11; Millipore Billerica, MA USA) followed by washing and incubating with goat anti-mouse rhodamine red. Coverslips were mounted and viewed at 60× magnification.
Prism software was used for statistical analysis (Orange County, CA USA). Data were analyzed by one-way analysis of variance or unpaired t test as appropriate.
To assess the effect of citrullinated fibronectin on synovial fibroblasts, we first prepared citrullinated fibronectin. We used the same method of in vitro citrullination as Chang and colleagues, who evaluated the binding of citrullinated fibronectin to β1 integrin . The citrullinated fibrinogen that caused increased macrophage activation, immunogenicity, and arthritogenicity was prepared using similar methods and reagents [10, 12]. In brief, fibronectin was incubated with buffer containing PAD or, as a control, buffer alone. As an additional control, we used fibronectin exposed to PAD that was heat-inactivated at 95°C. Heat-inactivated PAD does not have any detectable citrullinating activity and appears to stay in solution (data not shown).
Since citrullinated fibronectin has a reduced charge, we tested whether it coated plastic equally to untreated fibronectin. Plastic wells were incubated with PBS, fibronectin, or citrullinated fibronectin and washed with PBS. The amount of coated protein on the plastic was quantified and found to be equal for untreated and citrullinated fibronectin (Figure 1C).
Even more physiologically relevant would be an abnormality in adhesion seen with rheumatoid synovial fibroblasts. We therefore isolated synovial fibroblasts from patients with rheumatoid arthritis and repeated our attachment studies. As shown in Figure 5B, adhesion was delayed on citrullinated versus untreated fibronectin for rheumatoid synovial fibroblasts. We also evaluated cell spreading 3 hours after plating cells on fibronectin and citrullinated fibronectin. Rheumatoid synovial fibroblasts had impaired cell spreading and the cells displayed an abnormal morphology with extension of spindly processes on citrullinated fibronectin (Figure 5C). For both murine and human arthritic synovial fibroblasts, results on untreated fibronectin were similar to fibronectin that had been incubated with heat-inactivated PAD (data not shown).
One of the most harmful characteristics of synovial fibroblasts is their invasive capability. Cellular invasion requires cell adhesion, migration, and the formation of invasive structures that focally degrade matrix. We were able to show defects in adhesion and migration on citrullinated fibronectin (Figures 2, 3, and 5). We therefore wanted to assess whether citrullinated fibronectin also altered focal matrix degradation by arthritic synovial fibroblasts. To this end, we plated arthritic synovial fibroblasts from mice that overexpress TNFα on coverslips that had first been coated with fluorescent gelatin and then with either fibronectin or citrullinated fibronectin. After 4 hours, cells were fixed, permeabilized, and stained for cortactin, a marker of invadopodia. As shown in Figure 5D, some cells are able to focally degrade gelatin on either citrullinated or untreated fibronectin, although they tend to move over the course of the experiment and do not align perfectly with their areas of degradation. The percentage of cells that degraded matrix was calculated and was the same for citrullinated versus untreated fibronectin (Figure 5E). Similar results were seen for rheumatoid synovial fibroblasts but a much larger percentage of cells were degrading gelatin on either substrate, making it impossible to determine whether citrullinated fibronectin increased degradation. Of note, each degrading cell had a somewhat different pattern and density of degradation, but there were no differences in the degradation patterns on control versus citrullinated fibronectin.
In this article we provide the first report of how synovial fibroblasts behave on citrullinated matrix, specifically how they adhere, migrate, and invade in the presence of citrullinated fibronectin. Understanding the interactions between synovial fibroblasts and citrullinated fibronectin is important for understanding the pathophysiology of rheumatoid arthritis. Fibronectin mediates many synovial fibroblast functions and is citrullinated in rheumatoid arthritis. Moreover, synovial fibroblasts are part of the destructive process of rheumatoid arthritis. However, until this work, the role of citrullinated fibronectin in synovial fibroblast behavior was unknown.
We have shown that synovial fibroblasts have impaired adhesion (Figures 2 and 5) and migration (Figure 3) on citrullinated fibronectin. Previous work has shown that fibronectin enhances adhesion of synovial fibroblasts to the rheumatoid joint, potentially leading to joint destruction. Further, migration of synovial fibroblasts in the bloodstream may contribute to the spread of rheumatoid arthritis. Given this information, one could hypothesize that citrullination of fibronectin, as is seen in rheumatoid arthritis, might be a mechanism to prevent synovial fibroblast adhesion and joint destruction as well as to inhibit migration of synovial fibroblasts and arthritis spread. Since citrullination occurs in joints affected by other forms of inflammatory arthritis, it could be a general mechanism to limit joint destruction in the setting of inflammation. In this scenario, citrullination has protective functions, which would be a novel role for citrullination since, to date, most studies of proteins citrullinated in rheumatoid arthritis have suggested a harmful role. However, the alternative might also be true with poor adhesion of synovial fibroblasts leading to more metastasis of these cells and the spread of arthritis.
Directly investigating the role of citrullinated fibronectin in vivo is difficult since overexpressing or knocking out PAD2 and/or PAD4 would alter citrullination of many proteins and would not address fibronectin alone. However, such studies are warranted to address the role of citrullination in general since citrullination occurs in rheumatoid arthritis and since ACPAs are pathologic. Further, chemicals such as Cl-amidine are being studied for treating arthritis and it will be important to understand all the potential effects of this chemical and similar others. Finally, citrullination occurs in multiple inflammatory conditions [8, 9], in the lungs of smokers , and in different cancers , and there may be a common role for citrullination in all of these processes that is important to understand. This paper is one of the first steps in understanding the role of citrullination since fibronectin plays a critical role in the behavior of cells in inflammation and cancer.
We assessed whether citrullinated fibronectin would alter matrix degradation, a pathologic function of synovial fibroblasts in rheumatoid arthritis. We found no difference in the number of degrading cells in the presence of citrullinated versus untreated fibronectin. This was surprising to us. We had predicted either an increase in the number of degrading cells given the increased arthritogenicity of citrullinated collagen and fibrinogen, or a decrease in degrading cells due to the importance of fibronectin in matrix metalloprotease production and invadopodia stability. We performed additional experiments that showed no alteration in invasion through Matrigel invasion chambers incubated with PAD compared with untreated chambers (data not shown), but in those experiments multiple matrix proteins were probably citrullinated and the specific role of citrullinated fibronectin could not be addressed. However, those results are in agreement with the findings of our focal degradation experiments on fibronectin. Citrullination of fibronectin therefore appears to affect some synovial fibroblast behaviors but not all.
We also addressed the mechanism of impaired synovial fibroblast adhesion and migration on citrullinated fibronectin and found reduced phosphorylation of FAK and paxillin. This observation, in combination with the known decreased affinity of citrullinated fibronectin for β1 integrin , suggests that the impaired adhesion and migration is due to decreased binding of citrullinated fibronectin to integrins and subsequent decreased integrin-mediated signaling.
We tried to assess whether the canonical cell-binding RGD domain was involved with poor adhesion on citrullinated fibronectin. In competition experiments using RGD peptide to interfere with synovial fibroblast adhesion to fibronectin, however, we found that even high concentrations of RGD peptide did not significantly alter synovial fibroblast attachment in our assay. We were therefore unable to show lack of competition using a peptide with arginine replaced by citrulline (data not shown). Another domain known to mediate adhesion is the heparan sulfate binding domain. The major heparin binding sequences contain arginines , so this domain may be important for synovial fibroblast adhesion to fibronectin and impaired adhesion to citrullinated fibronectin. A general loss of tertiary structure with citrullination, as has been shown for other proteins , may also impair fibronectin's function and protein interactions and thus be responsible for altered synovial fibroblast behavior on citrullinated fibronectin. Altered tertiary structure or loss of arginines in portions of fibronectin not yet recognized to be involved with synovial fibroblast adhesion may be the most probable explanation for the observed cellular defects on citrullinated fibronectin since, to date, only five arginines in human fibronectin purified from the synovial fluid of two people with rheumatoid arthritis have been identified as citrullinated , none of which are in the RGD domain or the heparan sulfate binding domain.
One important caveat in interpreting our studies, however, is that the effects of in vitro citrullinated human plasma fibronectin by PAD from rabbit skeletal muscle on mouse and human synovial fibroblasts might be different from the effects of physiologic citrullination of fibronectin on synovial fibroblasts in the inflamed human joint. Nonetheless, the alterations of synovial fibroblast behavior on citrullinated fibronectin that we report here open the doors to future studies.
This is the first paper to show how citrullinated fibronectin, which is present in rheumatoid arthritis, alters the behavior of both normal and rheumatoid synovial fibroblasts. Given the pathologic role of ACPAs in rheumatoid arthritis, the presence of citrullination in inflammation and cancer, and the importance of communication between cells and extracellular matrix proteins, the implications of this work may be profound. However, further studies are needed to address the role of citrullinated fibronectin and other citrullinated extracellular matrix proteins in arthritis and other diseases both in vitro and in vivo.
anti-citrullinated protein antibody
bovine serum albumin
chemokine (C-X-C motif) ligand
Dulbecco's modified Eagle's medium
focal adhesion kinase
fetal bovine serum
- H & E:
hematoxylin and eosin
peptidyl arginine deiminase
tumor necrosis factor.
TNF transgenic mice (line 3647) were generously provided by Dr Edward Schwarz and permission for their use was generously provided by Dr George Kollias at the Alexander Fleming Biomedical Sciences Research Centre. Funding for this project was provided by the American College of Rheumatology Research and Education Foundation Rheumatology Scientist Development Award to MAS, National Institutes of Health (NIH) T32 HL007899 to MAS (PI John Sheehan, University of Wisconsin), and NIH R01 CA085862 to AH.
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