Open label study of escalating doses of oral treprostinil diethanolamine in patients with systemic sclerosis and digital ischemia: pharmacokinetics and correlation with digital perfusion
- Ami A Shah†1Email author,
- Elena Schiopu†2,
- Laura K Hummers1,
- Michael Wade3,
- Kristine Phillips2,
- Cynthia Anderson1,
- Robert Wise1,
- Francesco Boin1,
- James R Seibold4,
- Fredrick Wigley1 and
- Kristan D Rollins3
© Shah et al.; licensee BioMed Central Ltd. 2013
Received: 29 October 2012
Accepted: 18 April 2013
Published: 18 April 2013
Treprostinil diethanolamine is an innovative salt form of the prostacyclin analogue, treprostinil sodium, developed as an oral sustained release (SR) osmotic tablet. The availability of a formulation permitting convenient systemic delivery might have applicability to scleroderma vascular complications. We evaluated pharmacokinetics and perfusion in scleroderma patients with digital ischemia following escalating twice-daily doses of treprostinil diethanolamine SR.
Scleroderma patients with digital ulcers were enrolled in this dual-center, open-label, phase I pharmacokinetic study. Drug concentrations and perfusion, quantified by laser Doppler imaging, were measured over 12 hours at the 2 mg and 4 mg (or maximally tolerated) doses. Pharmacokinetic parameters were determined from individual plasma concentration versus time profiles using non-compartmental analysis methods. Digital perfusion and skin temperature were modeled as a function of log-transformed drug concentration and other covariates by performing repeated measures analyses using random effects models.
Nineteen scleroderma patients (84% female, 53% limited scleroderma) received treprostinil diethanolamine SR with dose titration up to 4 mg twice daily as tolerated. Peak concentrations (mean maximum plasma concentration (Cmax) = 1,176 and 2,107 pg/mL) occurred approximately 3.6 hours after dose administration, and overall exposure (under the plasma concentration-time curve from time 0 to 12 hours post dose (AUC0-12) = 7,187 and 12,992 hr*pg/mL) was linear between the 2 mg and 4 mg doses. Perfusion and digital skin temperature were positively associated with log-transformed plasma concentration at the 4 mg dose (P = 0.015 and P = 0.013, respectively). The most frequent adverse events were similar to those seen with prostacyclin analogues.
Oral treprostinil diethanolamine was effectively absorbed in patients with scleroderma. Drug administration was temporally associated with improved cutaneous perfusion and temperature. Treprostinil diethanolamine may provide a new therapeutic option for Raynaud's phenomenon and the peripheral vascular disease of scleroderma.
Treprostinil is a chemically stable analog of prostacyclin. The major pharmacological actions of treprostinil are direct vasodilation of pulmonary and systemic arterial vascular beds, inhibition of platelet aggregation, and inhibition of vascular smooth muscle cell proliferation [1–4]. Treprostinil, as the sodium salt, has shown clinical effectiveness in the treatment of pulmonary arterial hypertension (PAH) when administered by the continuous subcutaneous and intravenous (via infusion pump) and inhaled (via nebulizer device) routes of administration as the approved products Remodulin Injection and Tyvaso® Inhalation Solution, respectively . Treprostinil diethanolamine is an innovative salt form developed for oral delivery as a sustained release (SR) osmotic tablet. Both treprostinil sodium and treprostinil diethanolamine disassociate in blood and exist as the ionized, bioactive form of treprostinil.
Patients with diseases, such as systemic sclerosis (scleroderma, SSc), which involve perturbation of the peripheral vasculature, experience abnormalities in the blood vessels and mal-distribution of blood flow away from the nutritive capillaries that supply the skin. Raynaud's phenomenon is an episodic vasoconstriction of small arteries in response to cold or stress that occurs in more than 90% of patients with SSc . An associated small vessel obliterative vasculopathy frequently leads to the development of ischemic digital ulcers , a complication occurring in more than 40% of SSc patients . As a chemically stable tricyclic benzindine analog of prostacyclin, treprostinil inhibits platelet aggregation, induces vasodilation, and suppresses smooth muscle proliferation. Improvement in microvascular arterial blood flow and velocity in lower limbs and reduction in ischemic pain and healing of digital ulcers and other ischemic wounds has been observed with infused prostacyclin and prostacyclin analogs [9–19]. As an orally available formulation, treprostinil diethanolamine SR avoids the need for infusion devices and the associated demands and risks associated with the intravenous or subcutaneous route of delivery and may provide a treatment option for the peripheral vascular disease seen in SSc.
The aim of the present study was to determine the pharmacokinetic, safety and tolerability profiles following escalating twice-daily doses of treprostinil diethanolamine SR in patients with SSc. During this pharmacokinetic trial, we also quantified changes in digital perfusion by laser Doppler imaging and correlated levels of perfusion with plasma drug concentration.
The study was a two-center, open-label, pharmacokinetic study in adult subjects with scleroderma who were enrolled at the Johns Hopkins Scleroderma Center and the University of Michigan Scleroderma Program at Ann Arbor (http://ClinicalTrials.gov Identifier: NCT00848939). Approval from the Johns Hopkins Medicine Institutional Review Board and the University of Michigan Medical School Institutional Review Board for the study and written informed consent from subjects was obtained prior to performing any study-related procedures. This study was generally conducted in accordance with the ethical principles that have their origins in the Declaration of Helsinki and the ICH GCP E6 Good Clinical Practice guidance.
Male and female subjects were eligible for participation in the study based on the following criteria: subjects were older than 18 years of age, met American College of Rheumatology criteria  for SSc, and had either an active digital ulcer or history of digital ulceration occurring within the past six months and at least six to ten Raynaud's phenomenon episodes per week. Conventional therapies for SSc and digital ulcers, including calcium channel blockers, were permitted at stable doses throughout the study.
Exclusion criteria included an established diagnosis of PAH, low body weight (less than 40 kg), concomitant other connective tissue disease, postural hypotension or syncope, anemia (hemoglobin less than 75% of lower limit of normal), moderate to severe hepatic impairment or abnormal transaminases (greater than three times the upper limit of normal), intractable diarrhea, severe malabsorption, severe organ failure, cigarette smoking within six months, pregnancy, or lactation. Additionally, subjects who had an upper limb sympathectomy in the last year, or received treatment with a parenteral prostanoid within the previous three months, local botulinum toxin injection within one month, or systemic antibiotics to treat infected digital ulcers within two weeks of the baseline visit were excluded. Subjects receiving treatment with a phosphodiesterase inhibitor therapy except for erectile dysfunction were also excluded. Use of gemfibrozil, cyclophosphamide, glitazones, rifampin and grapefruit-containing products was not permitted over the course of the study. The investigators recorded other comorbid conditions and organ system involvement that did not meet these exclusion criteria.
Treprostinil diethanolamine SR was provided as 0.25 mg, 0.5 mg and 1 mg strengths for administration in the study. Oral dosing of treprostinil diethanolamine SR was initiated at 0.25 mg twice daily (BID) and the dose escalated in 0.25 mg increments every 48 hours at the evening dose, based on tolerability, up to a target dose of 4 mg BID (or maximum dose tolerated by each subject at the end of the eight-week escalation period). Subjects were instructed on the appropriate combination of tablets to administer for each dose and to take doses approximately every 12 hours within 10 to 15 minutes following a morning and evening meal containing approximately 500 calories.
Patients underwent 12-hour pharmacokinetic sampling upon reaching doses of 2 mg and 4 mg (or at the maximum achieved dose at the end of the titration period). Subjects who did not reach a dose of 2 mg BID during the study had assessments performed only at the end of study visit at their maximally tolerated dose. All pharmacokinetic blood sampling took place in the General Clinical Research Center (GCRC) and occurred after the subject had maintained the dose for at least five days. Subjects consumed a standardized breakfast (totaling approximately 500 calories) 10 to 15 minutes prior to the administration of treprostinil diethanolamine SR on pharmacokinetic sampling days.
Venous blood samples for pharmacokinetic assessments were obtained by venipuncture or in-dwelling cannula with a saline lock (no heparin). Eight venous blood samples of approximately 5 mL were collected in K3-ethylenediaminetetraacetic acid (EDTA) vacutainer tubes over the 12-hour dosing interval at the following time points: prior to dosing, and at 1, 2, 4, 6, 8, 10, and 12 hours post-dose for determination of treprostinil plasma concentrations.
Within one hour of blood collection, plasma was separated by centrifugation at 4°C for 10 to 15 minutes at 3,000 g, harvested and immediately stored frozen at -20 to -25°C until shipment on dry ice for analysis. Plasma samples were analyzed by Enthalpy Analytical, Inc. (Durham, NC, USA). Treprostinil concentrations were determined by a validated method using solid-phase extraction followed by ultra-performance liquid chromatography coupled with tandem mass spectrometry detection (HPLC/MS/MS) . The lower limit of quantitation was 10 pg/mL using a 150 µL aliquot of human plasma, and the linear concentration range for the calibration curve was 10 to 5,000 pg/mL. The accuracy and precision were determined during validation by analyzing the quality control (QC) sample concentrations to ensure that the calibration standards that were analyzed were suitable for generating calibration curves. The acceptance criteria required the average accuracy be within ±15% and the precision should be ≤15% for low, mid, high, and dilution QC levels. For the lower limit of quantification QC level, the average accuracy needed to be within ±20% and the precision had to be ≤20%. Greater than or equal to half of the QC samples at any given concentration had to meet accuracy acceptance criteria. In addition, greater than or equal to two-thirds of all QC samples had to meet accuracy acceptance criteria.
Pharmacokinetic parameters were determined from individual plasma concentration versus time profiles using non-compartmental analysis methods and WinNonlin version 5.2.1 (Pharsight Corporation, Mountain View, CA, USA). Pharmacokinetic analysis included determination of the observed maximum plasma concentration (Cmax), time to maximum concentration (tmax), area under the plasma concentration-time curve from time zero to the last time point with measurable drug concentration as calculated by the linear trapezoidal method (AUC0-t), area under the plasma concentration-time curve from time zero to 12 hours post dose (AUC0-12), and apparent terminal elimination half-life (t1/2). Pharmacokinetic parameters were summarized by calculating the mean, median and coefficient of variation (CV%) by dose regimen. Pharmacokinetic parameters derived from subjects' treprostinil concentration profiles at doses other than 2 mg BID or 4 mg BID were excluded from the statistical summary and comparisons. Actual sampling times were used in the calculations of pharmacokinetic parameters. Plasma concentrations that were below the lower limit of quantitation were set to zero when calculating summary statistics for plasma concentration at a given nominal time point, except when a single value fell between two measurable concentrations, then this value was set to be missing.
Dose proportionality in treprostinil AUC0-12 and Cmax following repeat doses of 2 mg and 4 mg BID was assessed by analysis of covariance (ANCOVA). Cmax and AUC0-12 were dose normalized (to 1 mg) and loge-transformed values were analyzed using a mixed-effect model by restricted maximum likelihood (REML) using SAS Proc Mixed, with dose regimen as a fixed effect and subject as a random effect. The least squares mean (LSM) difference in the parameter estimates between the two dose regimens and the 90% confidence interval (CI) for the difference were calculated. The difference and CI were then transformed back to the original scale to provide an estimate of the geometric LSM (GLSM) and 90% CI as the parameter ratio, 4 mg BID versus 2 mg BID.
Measurement of digital perfusion and skin temperature
Subjects enrolled at the Johns Hopkins Scleroderma Center also had serial measurements of digital perfusion performed at each pharmacokinetic visit pre-dose and 1, 2, 4, 6, 8, 10 and 12 hours post-dose. Laser Doppler imaging was performed using the Moor Instruments LDI2-IR imaging system (Axminster, Devon UK). Perfusion was expressed as arbitrary perfusion units (PU). Laser Doppler imaging was performed in a quiet, temperature controlled room (20 to 23°C) following an acclimation period of 15 minutes prior to initiation of testing. Digital skin temperature was measured immediately prior to each laser Doppler imaging scan by affixing a thermistor (YSI, Yellow Springs, OH, USA) to the pad of the third distal phalanx of the hand under study.
Mean perfusion of the middle three fingers of both hands and mean digital skin temperatures at pre-dose trough (time 0) were compared to 12 hours post-dose for each pharmacokinetic visit using the paired t-test after the normality of these variables' distributions was confirmed by the Shapiro-Wilk test. Repeated measures analyses using random effects models were performed using perfusion and skin temperature as dependent variables of interest and perfusion obtained at drug trough, log-transformed drug concentration, visit number, hour of Doppler assessment, and left versus right hand as potential explanatory variables. Covariates with P-values <0.15 were retained in the statistical model. All data were used across time points, visits, and hands. AUC for the perfusion parameter was calculated using the trapezoidal rule on the observed perfusion values.
Tolerability and safety
Throughout all treatment phases, vital signs, the occurrence of adverse events and use of concomitant medication was monitored for each subject. The rating of intensity of adverse events was by subject report and physician determination. Samples for clinical laboratory tests were collected prior to the screening visit and at study exit. Treatment-emergent clinically significant abnormalities in vital signs and clinical laboratory data were examined.
A total of 20 patients with SSc (ten at each center) were enrolled between March and December 2009. Nineteen subjects received at least one dose of study drug, and 16 subjects completed the eight week study in its entirety. Four subjects discontinued the study prematurely. Two subjects discontinued due to adverse events, one subject was withdrawn due to protocol violation, and another subject was lost to follow-up after undergoing the baseline visit. Two of these subjects withdrew after the first pharmacokinetic visit, and two subjects withdrew prior to undergoing any pharmacokinetic assessments.
Summary of Baseline Characteristics.
Number = 19
Age in years, mean (SD)
Gender: Male/Female (number)
Race: Caucasian/African American (number)
Weight in kg, mean (SD)
BMI in kg/m2, mean (SD)
SSc subtype, number
Duration of disease in years, mean (SD)
Organ involvement, number
Autoantibody status, number positive
Seventeen subjects reached a 2 mg BID dose, and 12 subjects achieved the 4 mg BID dose and underwent pharmacokinetic assessments. At the end of the eight week dose escalation period, four subjects completed the study at a maximum tolerated dose less than 4 mg BID: one subject each completed the study at 1 mg BID, 2 mg BID, 2.5 mg BID and 0.5 mg BID. The overall average duration of exposure in the study was 47.7 days (range 15 to 62 days), with an overall mean of 21 and 42 days to reach the 2 mg BID and 4 mg BID doses levels, respectively.
Pharmacokinetic parameters of treprostinil in SSc patients following dose escalation to 2 mg and 4 mg BID.
2 mg BID
(Number = 17)
4 mg BID
(Number = 12)
Ninety percent CIs for the GLSM ratios for dose-normalized AUC0-12 (ratio 1.10; 90% CI 0.97 to 1.25) and Cmax (ratio 1.03; 90% CI 0.89 to 1.19) demonstrated dose proportionality between the 2 mg and 4 mg BID regimens.
Digital perfusion and skin temperature
Of the 10 subjects enrolled at the Johns Hopkins Scleroderma Center, nine subjects underwent a laser Doppler imaging assessment upon reaching a 2 mg BID dose (first pharmacokinetic PK) visit). Pre-dose perfusion data were not available in one subject due to laser Doppler device malfunction. Eight subjects participated in laser Doppler imaging assessment at the end of study (second PK) visit, six subjects at the 4 mg BID dose and one subject each at the 0.5 mg BID and 1 mg BID doses, respectively. Average time on therapy at the first and second PK visits was 26 ± 4 days and 54 ± 4 days, respectively.
Safety and tolerability
The most frequent adverse events considered probably or possibly related to the study drug following dose escalation (up to 4 mg BID, or maximally tolerated dose) of repeated twice daily doses of treprostinil diethanolamine SR were headache (17 subjects; 89%), diarrhea (nine subjects; 47%), nausea (eight subjects; 42%), fatigue (four subjects; 21%), vomiting (four subjects; 21%), flushing (three subjects; 16%) and jaw pain (three subjects; 16%). There were no serious adverse events or deaths reported during this study. The majority of adverse events were reported to be either mild or moderate in intensity. Adverse events attributed to the study drug rated as severe were headache, reported by four subjects, and nausea, jaw pain and muscle spasms, each reported by one subject. Two subjects permanently discontinued the study drug due to adverse events. One subject discontinued due to severe headache after reaching a dose of 2 mg BID. A second subject discontinued the study at 3 mg BID, following a dose reduction after exceeding the target dose and reaching 4.25 mg BID, reporting myalgia (mild), nausea (mild) and vomiting (mild). There were no clinically relevant effects on hematology, biochemistry or urinalysis parameters.
The primary objective of this study was to characterize treprostinil pharmacokinetics in patients with SSc following repeat doses of treprostinil diethanolamine SR up to a maximum dose of 4 mg BID. Our data show that the oral SR formulation of treprostinil diethanolamine was effectively absorbed and that improvements in skin perfusion and temperature were temporally associated with drug levels during short-term (eight-week) treatment. This study demonstrated that oral administration of treprostinil diethanolamine resulted in systemic treprostinil exposure similar to that achieved with parenteral treprostinil doses of 10 to 20 ng/kg/minute [21–24]. This is the first study to demonstrate that cutaneous perfusion may improve with an oral prostacyclin formulation. Improvements in skin perfusion and temperature were detected at the second PK visit (4 mg dose), but not the first PK visit (2 mg dose). This difference may suggest a dose-response relationship, the effect of vascular remodeling with a longer duration of treprostinil therapy, or a statistical anomaly.
Parenteral treprostinil therapy has been utilized in previous studies in SSc patients with digital ulcer disease [16–19]. Due to the delivery complexities associated with this therapy an alternative was sought to enhance patient acceptance and to improve treatment options for this population. Treprostinil diethanolamine is an innovative salt form of treprostinil that has been developed and formulated into a SR osmotic tablet oral dosage formulation to provide near zero-order release of treprostinil over the majority of a 12-hour period. In the plasma at physiological pH, treprostinil exists in the ionized state. Once ionized, treprostinil will freely associate with the predominant counter-ion in the plasma, predominantly sodium. The bioactive species derived from either salt (treprostinil sodium or treprostinil diethanolamine) exists in the same biologically active form in the plasma as treprostinil; thus, treprostinil diethanolamine is expected to retain the bioactivity and safety profile of the approved infused treprostinil sodium.
Consistent with established clinical use of prostacyclins, treprostinil diethanolamine SR requires dose titration based on signs and symptoms of disease and occurrence of adverse events. Pharmacokinetic sampling was selected at the 2 mg BID and 4 mg BID doses in this study, since they represented doses that could reasonably be achieved within an eight week escalation period. Consistent with studies in PAH patient populations, treprostinil diethanolamine SR provided plasma concentrations over the 12 hour dosing period supporting twice daily dosing in SSc patients. A linear relationship between dose and plasma concentration was observed at the 2 mg BID and 4 BID doses in SSc patients and there was no unexpected accumulation of treprostinil.
In our study following dose escalation to 2 mg BID and 4 mg BID with oral treprostinil diethanolamine SR, an increase in digital perfusion was observed with increased treprostinil blood concentrations, suggesting a dose-response relationship. After controlling for baseline perfusion, hour of assessment, and hand under study, plasma treprostinil concentration was a significant predictor of perfusion and digital skin temperature at the end of study visit. While these data show effective systemic drug absorption following oral delivery and temporally improved blood flow, further investigation with a larger sample size and a control population is needed to confirm this association, to investigate the contribution of other potentially relevant covariates to perfusion, to correct for individual subjects' natural perfusion variations, and to evaluate for dose effects.
During this study, treprostinil diethanolamine SR tablets were administered with a standardized 500 calorie meal. A recent study demonstrated that administration of treprostinil diethanolamine SR tablets following a 250 calorie meal or a 250 calorie liquid meal replacement supplement (Ensure®) did not have a clinically meaningful effect on the extent of treprostinil diethanolamine SR bioavailability or plasma exposure . Smaller meal sizes may be required in some scleroderma patients as a result of esophago-gastric involvement of their disease, and this would not be anticipated to impact dosing in those patients. Bosentan and sildenafil are therapeutic agents used for the treatment of PAH and in Raynaud's phenomenon and digital ulcer disease in SSc [26–28]. No pharmacokinetic interaction was observed when these agents were co-administered with treprostinil diethanolamine SR [29, 30].
The results of this study demonstrated that oral therapy with treprostinil diethanolamine SR initiated at 0.25 mg BID and gradually increased in stepwise 0.25 mg BID increments was reasonably well tolerated in subjects with SSc, and the overall adverse event profile was similar to that known to be associated with prostacyclin and its analogues in other patient populations . The frequency of adverse event reporting was not unexpected as investigators were instructed to continue to dose titrate in the absence of adverse events up to the target dose or a subject's maximally tolerated dose. Dose escalation was initiated in 0.25 mg BID increments every 48 hours. The titration rate was slowed at the investigator's discretion based upon individual subject tolerability. Modulation of the dose escalation rate and increasing the interval between dose increases was an effective strategy for managing adverse events. The most common adverse events reported with too fast of a titration rate or too large of a dose increase are headache, nausea, flushing, diarrhea and vomiting. Although an oral dosage form, management of treprostinil diethanolamine SR dosing closely follows experience with infused prostacyclins. Consistent with established clinical use of prostacyclins, treprostinil diethanolamine SR requires dose titration based on signs and symptoms of disease and occurrence of adverse events. Due to the large inter-patient variability in peak plasma concentration and exposure, selection of an individual dosing regimen may be required in SSc patients to titrate treprostinil diethanolamine SR to achieve clinical benefit and balance adverse effects of prostacyclins, much the same as with parenteral forms.
Currently prostacyclin analogues are administered via intravenous or subcutaneous routes in scleroderma patients with vascular complications. Our data show that the oral SR formulation of treprostinil diethanolamine was effectively absorbed in patients with SSc, was associated with improved cutaneous perfusion and temperature with short-term treatment, and may provide a new therapeutic option for Raynaud's phenomenon and the peripheral vascular disease of SSc.
area under the plasma concentration-time curve
area under the plasma concentration-time curve from time 0 to 12 hours post dose
maximum plasma concentration
coefficient of variation
geometric least squares mean
least squares mean
pulmonary arterial hypertension
time to maximum concentration
apparent terminal elimination half-life.
This study was sponsored by United Therapeutics Corporation (Research Triangle Park, NC, USA). Additional Johns Hopkins University funding sources include the Scleroderma Research Foundation, the American College of Rheumatology Research and Education Foundation's Clinical Investigator Fellowship Award and Bridge Funding Award [to AAS], and the National Institutes of Health (NIH) (1K23AR061439 to AAS, 1K23AR052742 to LKH, 1K23AR055667 to FB, 1K23AR060241 to KP, 1P50 HL084946-01 to FMW and LKH). Additional University of Michigan funding sources include the Jonathan and Lisa Rye Scleroderma Research Fund, the Marvin and Betty Danto Scleroderma Research Fund, and the Scleroderma Program at the University of Michigan. This publication was made possible by grants (UL1 RR 025005 Johns Hopkins; UL1 RR 024986 University of Michigan) from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH) and NIH Roadmap for Medical Research.
- Clapp LH, Finney P, Turcato S, Tran S, Rubin LJ, Tinker A: Differential effects of stable prostacyclin analogs on smooth muscle proliferation and cyclic AMP generation in human pulmonary artery. Am J Respir Cell Mol Biol. 2002, 26: 194-201. 10.1165/ajrcmb.26.2.4695.View ArticlePubMedGoogle Scholar
- Tuder RM, Zaiman AL: Prostacyclin analogs as the brakes for pulmonary artery smooth muscle cell proliferation: is it sufficient to treat severe pulmonary hypertension?. Am J Respir Cell Mol Biol. 2002, 26: 171-174. 10.1165/ajrcmb.26.2.f230.View ArticlePubMedGoogle Scholar
- Martindale: the complete drug reference: Treprostinil. Micromedex® Healthcare Series. 2003(a), London: Pharmaceutical PressGoogle Scholar
- Vachiery JL, Naeije R: Treprostinil for pulmonary hypertension. Expert Rev Cardiovasc Ther. 2004, 2: 183-191. 10.1586/14779072.2.2.183.View ArticlePubMedGoogle Scholar
- Remodulin (treprostinil) injection [package insert]. 2011, Research Triangle Park, NC: United Therapeutics Corporation
- Medsger, Masi AT, Rodnan GP, Benedek TG, Robinson H: Survival with systemic sclerosis (scleroderma). A life-table analysis of clinical and demographic factors in 309 patients. Ann Intern Med. 1971, 75: 369-76. 10.7326/0003-4819-75-3-369.View ArticlePubMedGoogle Scholar
- Rodnan GP, Myerowitz RL, Justh GO: Morphologic changes in the digital arteries of patients with progressive systemic sclerosis (scleroderma) and Raynaud phenomenon. Medicine (Baltimore). 1980, 59: 393-408.View ArticleGoogle Scholar
- Khimdas S, Harding S, Bonner A, Zummer B, Baron M, Pope J: Associations with digital ulcers in a large cohort of systemic sclerosis: results from the Canadian scleroderma research group registry. Arthritis Care Res (Hoboken). 2011, 63: 142-149. 10.1002/acr.20336.View ArticleGoogle Scholar
- Rademaker M, Cooke ED, Almond NE, Beacham JA, Smith RE, Mant TG, Kirby JD: Comparison of intravenous infusions of iloprost and oral nifedipine in treatment of Raynaud's phenomenon in patients with systemic sclerosis: a double blind randomised study. BMJ. 1989, 298: 561-564. 10.1136/bmj.298.6673.561.View ArticlePubMedPubMed CentralGoogle Scholar
- Torley HI, Madhok R, Capell HA, Brouwer RM, Maddison PJ, Black CM, Englert H, Dormandy JA, Watson HR: A double blind, randomised, multicentre comparison of two doses of intravenous iloprost in the treatment of Raynaud's phenomenon secondary to connective tissue diseases. Ann Rheum Dis. 1991, 50: 800-804. 10.1136/ard.50.11.800.View ArticlePubMedPubMed CentralGoogle Scholar
- Wigley FM, Seibold JR, Wise RA, McCloskey DA, Dole WP: Intravenous iloprost treatment of Raynaud's phenomenon and ischemic ulcers secondary to systemic sclerosis. J Rheumatol. 1992, 19: 1407-1414.PubMedGoogle Scholar
- Wigley FM, Wise RA, Seibold JR, McCloskey DA, Kujala G, Medsger TA, Steen VD, Varga J, Jimenez S, Mayes M, Clements PJ, Weiner SR, Porter J, Ellman M, Wise C, Kaufman LD, Williams J, Dole W: Intravenous iloprost infusion in patients with Raynaud phenomenon secondary to systemic sclerosis. A multicenter, placebo-controlled, double-blind study. Ann Intern Med. 1994, 120: 199-206. 10.7326/0003-4819-120-3-199402010-00004.View ArticlePubMedGoogle Scholar
- Clifford PC, Martin MF, Sheddon EJ, Kirby JD, Baird RN, Dieppe PA: Treatment of vasospastic disease with prostaglandin E1. Br Med J. 1980, 281: 1031-1034. 10.1136/bmj.281.6247.1031.View ArticlePubMedPubMed CentralGoogle Scholar
- Pope J, Fenlon D, Thompson A, Shea B, Furst D, Wells G, Silman A: Iloprost and cisaprost for Raynaud's phenomenon in progressive systemic sclerosis. Cochrane Database Syst Rev. 2000, 2: CD000953-PubMedGoogle Scholar
- Mohler ER, Klugherz B, Goldman R, Kimmel SE, Wade M, Sehgal CM: Trial of a novel prostacyclin analog, UT-15, in patients with severe intermittent claudication. Vasc Med. 2000, 5: 231-237.View ArticlePubMedGoogle Scholar
- Engel G, Rockson SG: Treprostinil for the treatment of severe digital necrosis in systemic sclerosis. Vasc Med. 2005, 10: 29-32. 10.1191/1358863x05vm579cr.View ArticlePubMedGoogle Scholar
- Fernandez B, Strootman D: The prostacyclin analog, treprostinil sodium, provides symptom relief in severe Buerger's disease--a case report and review of literature. Angiology. 2006, 57: 99-102. 10.1177/000331970605700114.View ArticlePubMedGoogle Scholar
- Berman S, Quick R, Yoder P, Voigt S, Strootman D, Wade M: Treprostinil sodium (Remodulin), a prostacyclin analogue, in the treatment of critical limb ischemia: open-label study. Vascular. 2006, 14: 142-148. 10.2310/6670.2006.00028.View ArticlePubMedGoogle Scholar
- Chung L, Fiorentino D: A pilot trial of treprostinil for the treatment and prevention of digital ulcers in patients with systemic sclerosis. J Am Acad Derm. 2006, 54: 880-882. 10.1016/j.jaad.2006.02.004.View ArticlePubMedGoogle Scholar
- Preliminary criteria for the classification of systemic sclerosis (scleroderma): Subcommittee for scleroderma criteria of the American Rheumatism Association diagnostic and therapeutic criteria committee. Arthritis Rheum. 1980, 23: 581-590. 10.1002/art.1780230510.View ArticleGoogle Scholar
- Laliberte K, Arneson C, Jeffs R, Hunt T, Wade M: Pharmacokinetics and steady-state bioequivalence of treprostinil sodium (Remodulin®) administered by the intravenous and subcutaneous route to normal volunteers. J Cardiovasc Pharmacol. 2004, 44: 209-214. 10.1097/00005344-200408000-00010.View ArticlePubMedGoogle Scholar
- White RJ, Allen R, Torres F, Jerjes C, Pulido T, Carroll J, Yehle D, Laliberte K, Mottola D, Galie N, Simmoneau G, Tapson VF: Sustained plasma concentrations of treprostinil following chronic dosing in patients with pulmonary arterial hypertension [abstract]. Am J Respir Crit Care Med. 2009, 179: A3360-Google Scholar
- Wade M, Baker FJ, Roscigno R, DellaMaestra W, Arneson CP, Hunt TL, Lai AA: Pharmacokinetics of treprostinil sodium administered by 28-day chronic continuous subcutaneous infusion. J Clin Pharmacol. 2004, 44: 503-509. 10.1177/0091270004264638.View ArticlePubMedGoogle Scholar
- McSwain CS, Benza R, Shapiro S, Hill N, Schilz R, Elliott CG, Zwicke DL, Oudiz RJ, Staszewski JP, Arneson CP, Wade M, Zaccardelli D, McLaughlin V: Dose proportionality of treprostinil sodium administered by continuous subcutaneous and intravenous infusion. J Clin Pharmacol. 2008, 48: 19-25. 10.1177/0091270007309708.View ArticlePubMedGoogle Scholar
- Lim A, White RJ, Walker S, Kates J, Laliberte K: The effect of different meal compositions on the pharmacokinetics of treprostinil diethanolamine in healthy volunteers [abstract]. Chest. 2010, 138: 366A-10.1378/chest.10191.View ArticleGoogle Scholar
- Korn JH, Mayes M, Matucci Cerinic M, Rainisio M, Pope J, Hachulla E, Rich E, Carpentier P, Molitor J, Seibold JR, Hsu V, Guillevin L, Chatterjee S, Peter HH, Coppock J, Herrick A, Merkel PA, Simms R, Denton CP, Furst D, Nguyen N, Gaitonde M, Black C: Digital ulcers in systemic sclerosis: Prevention by treatment with bosentan, an oral endothelin receptor antagonist. Arthritis Rheum. 2004, 50: 3985-3993. 10.1002/art.20676.View ArticlePubMedGoogle Scholar
- Gore J, Silver R: Oral sildenafil for the treatment of Raynaud's phenomenon and digital ulcers secondary to systemic sclerosis. Ann Rheum Dis. 2005, 64: 1387-10.1136/ard.2004.034488.View ArticlePubMedPubMed CentralGoogle Scholar
- Brueckner CS, Becker MO, Kroencke T, Huscher D, Scherer HU, Worm M, Burmester G, Riemekasten G: Effect of sildenafil on digital ulcers in systemic sclerosis: analysis from a single centre pilot study. Ann Rheum Dis. 2010, 69: 1475-1478. 10.1136/ard.2009.116475.View ArticlePubMedPubMed CentralGoogle Scholar
- Gotzkowsky SK, Dingemanse J, Lai A, Mottola D, Laliberte K: Lack of a pharmacokinetic interaction between oral treprostinil and bosentan in healthy adult volunteers. J Clin Pharmacol. 2010, 50: 829-834. 10.1177/0091270009351173.View ArticlePubMedGoogle Scholar
- Gotzkowsky SK, Laliberte K, Goetz B, Mottola D: Lack of a pharmacokinetic drug interaction following co-administration of UT-15C (treprostinil diethanolamine) SR and Revatio™ (sildenafil citrate) in healthy volunteers [poster]. San Francisco, CA, Presented at: American Thoracic Society; May 18-23, 2007
- McLaughlin VV, Archer SL, Badesch DB, Barst RJ, Farber HW, Lindner JR, Mathier MA, McGoon MD, Park MH, Rosenson RS, Rubin LJ, Tapson VF, Varga J, American College of Cardiology Foundation Task Force on Expert Consensus Documents, American Heart Association, American College of Chest Physicians, American Thoracic Society, Pulmonary HypertensionAssociation: ACCF/AHA 2009 expert consensus document on pulmonary hypertension: a report of the American College of Cardiology Foundation Task Force on expert consensus documents and the American Heart Association developed in collaboration with the American College of Chest Physicians; American Thoracic Society, Inc.; and the Pulmonary Hypertension Association. J Am Coll Cardiol. 2009, 53: 1573-1619. 10.1016/j.jacc.2009.01.004.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.