Epistasis with HLA DR3 implicates the P2X7 receptor in the pathogenesis of primary Sjögren's syndrome
- Susan Lester1,
- Leanne Stokes2, 3,
- Kristen K Skarratt2,
- Ben J Gu2, 4,
- Kathy L Sivils5,
- Christopher J Lessard5,
- James S Wiley†2, 4 and
- Maureen Rischmueller†1, 6Email author
© Lester et al.; licensee BioMed Central Ltd. 2013
Received: 6 November 2012
Accepted: 2 June 2013
Published: 2 June 2013
The aim of this study was to examine the association between functional polymorphisms in the pro-inflammatory P2X7 receptor and the Ro/La autoantibody response in primary Sjögren's syndrome (pSS).
Twelve functional P2RX7 polymorphisms were genotyped in 114 pSS patients fulfilling the Revised American-European Consensus Criteria for pSS, and 136 controls. Genotyping of the A1405G (rs2230912) polymorphism was performed on a replication cohort consisting of 281 pSS patients and 534 controls. P2X7 receptor function in lymphocytes and monocytes was assessed by measurement of ATP-induced ethidium+ uptake. Serum IL-18 levels were determined by ELISA.
The minor allele of P2RX7 A1405G is a tag for a common haplotype associated with gain in receptor function, as assessed by ATP-induced ethidium+ uptake. A positive association between 1405G and anti-Ro±La seropositive pSS patients was observed in Cohort 1. Although not replicated in Cohort 2, there was a consistent, significant, negative epistatic interaction effect with HLA-DR3 in seropositive pSS patients from both cohorts, thereby implicating this gain of function variant in the pathogenesis of pSS. Serum IL-18 was elevated in seropositive pSS patients, but was not influenced by P2RX7 A1405G.
The P2RX7 1405G gain-of-function haplotype may be a risk factor for seropositive pSS in a subset of subjects who do not carry HLA risk alleles, but has no effect in subjects who do (epistasis). Potential mechanisms relate to autoantigen exposure and inflammatory cytokine expression. The observed elevation of IL-18 levels is consistent with P2X7 receptor activation in seropositive pSS patients. Collectively these findings implicate P2X7 receptor function in the pathogenesis of pSS.
Primary Sjögren's syndrome (pSS) is a systemic autoimmune disease characterized by lymphocytic and monocytic infiltration into the salivary and lacrimal glands with inflammation, destruction of acini and failure of exocrine secretion. A wide range of extraglandular features also manifest in a subset of patients. Autoantibodies targeting the Ro and La components of a ribonucleoprotein (RNP) complex are highly specific for pSS and constitute one of the classification criteria for this disease . Once considered an epiphenomenon, these autoantibodies are now recognized to be involved in the systemic inflammation of pSS .
A substantial polygenic component underlies susceptibility to the Ro/La autoantibody response in pSS [3, 4], however the strongest individual association is observed with HLA-DRB1*03:01 (DR3, Chr 6p21.3) . Additional genes are also likely to be involved, and one candidate is the P2X7 receptor (Chr 12q24.31), whose chromosomal location lies within mapped human and murine systemic lupus erythematosus (SLE) susceptibility loci . The purinergic P2X7 receptor has been implicated in several murine models of autoimmune disease, including experimental autoimmune encephalomyelitis , rheumatoid arthritis (RA)  and autoimmune hepatitis .
The P2X7 receptor belongs to a two-transmembrane family of receptors, which are trimeric in the membrane and are attached via non-muscle myosin to the actin cytoskeleton [10–12]. Activation of P2X7, in response to the binding of extracellular ATP, opens a non-selective cation channel allowing an immediate influx of Ca2+ and Na+, and efflux of K+ ions. Continued activation, over tens of seconds, allows a larger permeability state to develop (termed pore formation), which is accompanied by extensive membrane blebbing, and ultimately cell death, with features of both apoptosis and necrosis [13, 14]. Extracellular ATP is normally maintained at low nanomolar levels by a ubiquitous ecto-ATPDase (CD39), although in an inflammatory milieu, ATP may be released from dead or injured cells, as well as by cell swelling or from autonomic nerves .
The P2X7 receptor is found on many cells and tissues throughout the body. The receptor is most highly expressed on cells of the macrophage lineage, including dendritic cells and microglia [13, 16], and both P2X7 receptors and HLA Class II molecules are upregulated in parallel when monocytes differentiate to these mature cell types. P2X7 receptors are also abundant on both acinar and ductal cells from submandibular and other salivary glands [17, 18]. A special role for this receptor in salivary glands has been highlighted by its direct involvement in the regulation of fluid secretion [19, 20]. In cells of the monocyte-macrophage lineage, there is much evidence that P2X7 is pro-inflammatory [16, 21], and P2X7 gene-deleted mice show major reductions in cytokine responses to various inflammatory stimuli . Activation of the P2X7 receptor results in maturation and release of the pro-inflammatory cytokines, IL-1β and IL-18 [21–23], a response that has been linked to formation of the cryopyrin (NALP3) inflammasome and subsequent caspase-1 activation [21, 24]. Elevated serum levels of both IL-1β and IL-18 have been reported in pSS [25, 26], and these cytokines have been implicated in salivary gland pathology in both mouse models  and human disease [28, 29].
There is considerable variability in P2X7 function between individuals, which can be largely attributed to polymorphisms of the P2RX7 gene. To date, one dozen polymorphisms have been identified in P2RX7, which alter receptor function [30, 31]. We and others have shown that polymorphisms that increase or decrease P2X7 receptor function confer a corresponding increase or decrease in ATP-stimulated secretion of IL-1β or IL-18 from monocytes primed with lipopolysaccharide [31–33]. We have now examined these functional P2RX7 SNPs in patients with pSS, and have identified an epistatic interaction between the major P2RX7 gain-of-function haplotype and HLA-DR3 in patients with seropositive pSS.
Materials and methods
Cohort 1 included 114 Caucasian pSS patients (88% female) from the South Australian Sjögren's Syndrome research registry, and 136 Caucasian population-based controls. The replication cohort (cohort 2) included 281 Caucasian pSS patients (95% female) obtained from the Oklahoma Medical Research Foundation (OMRF) plus 534 controls.
All patients met the revised 2002 American-European consensus research classification criteria for pSS . Anti-Ro±La status was performed as part of routine diagnosis, and was available for all patients. Autoantibody testing using recombinant protein was performed by ELISA in cohort 1 , and by immunodiffusion in cohort 2 . The majority of patients were autoantibody-positive, with 82% of pSS patients from the first cohort, and 71% of pSS patients from the second cohort positive for anti-Ro±La.
HLA DR3 status was available for all patients and controls for cohort 1 (medium resolution DRB1 genotyping performed by the Tissue Typing Laboratory, Adelaide Red Cross Blood Transfusion Service, South Australia), and for the majority of pSS patients from cohort 2 (a DR3 sequence-specific priming assay). A DR3 proxy SNP, rs2187668 , was used for analyses of the controls from cohort 2. The use of the minor allele of rs2187668 as a proxy for DR3 was validated against individuals with known DR3 status in each cohort, with observed kappa agreements of 0.95, and 0.92 respectively (data not shown).
The study was approved by the Human Ethics Committees of The Queen Elizabeth Hospital, the Sydney West Area Health Service and OMRF, in accordance with the Helsinki Declaration, and all participants gave informed, written consent.
P2RX7 SNPs genotyped in pSS patients and normal subjects
Intron 1 splice site
Genotype data for the P2X7 A1405G SNP (rs2230912) and the DR3 proxy, rs2187668 were obtained for cohort 2 using the Illumina OMNI-1 Quad array (San Diego, CA, USA) following manufacturer's protocols. These subjects were also evaluated for possible population stratification using principal component analysis implemented in Eiganstrat  utilizing approximately 30,000 independent SNPs (r2 <0.20) scattered thought the genome. Samples were plotted alongside the HapMap populations to determine which ones were genetic outliers.
Serum IL-18 assays
Serum IL-18 levels were assayed by sandwich ELISA (Human IL-18 ELISA Kit, Medical and Biological Laboratories Company Limited, Nagaoya, Japan, catalogue number 7620), according to the manufacturer's instructions.
P2X7 function measured by ATP-induced ethidium uptake (pore assay)
Peripheral venous blood was obtained from healthy volunteers and mononuclear cells were isolated by Ficoll-Paque gradient centrifugation. P2X7 receptor function was measured by the ATP-induced ethidium+ uptake in a mixed cell (B and T lymphocytes and monocytes) suspension using time-resolved two-colour flow cytometry . Briefly, mononuclear cells were labelled with a phycoerythrin (PE)-conjugated monoclonal antibody to CD3 and an allophycocyanin (APC)-conjugated monoclonal antibody to CD14 (Dako). Ethidium+ (25 μM) was added to a cell suspension maintained at 37°C with stirring in a time-zero module, and 1 mM ATP was added 40 s later. The cells were analysed at 1000 events/s on a FACSCalibur flow cytometer and gated by forward and side scatter and by cell type-specific monoclonal antibody. The linear mean channel of fluorescence intensity for each gated subpopulation over successive 10-s intervals was analysed by WinMDI version 2.8 software  and plotted against time to estimate the maximum rate of ethidium+ uptake. The linear portion of the ethidium+ uptake plot was used for slope calculations over a period of 1 minute.
Differences in allele frequencies between patients and controls were analysed by logistic regression using the default additive model for allele coding and odds ratios (OR) were derived from exponentiation of the regression coefficients. Epistasis was analysed using a two-factor interaction logistic regression model, with the significance of the epistasis determined by the significance of the coefficient for the interaction term. Analyses for cohort 2 were ancestry-adjusted by the inclusion of the principal components as covariates. Serum IL-18 levels were log-normally distributed and were therefore analysed by lognormal regression and data presented as medians. These analyses were performed using Statistica v6.1 (StatSoft Inc, Tulsa, Oklahoma, USA). Haplotype inference was performed using Phase version 2 software .
Functional P2RX7polymorphism in pSS patients and population based controls
Frequency of the minor allele of P2RX7 A1405 G allele in pSS patients and controls
Odds ratio (95% CI)
1.46 (0.91, 2.34)
1.78 (1.09, 2.92)
1.07 (0.79, 1.45)b
1.35 (0.87, 2.10) b
0.96 (0.68, 1.36) b
The minor allele of P2RX7A1405G exhibits a negative epistatic interaction with HLA DR3 in Ro±La seropositive pSS patients
We further investigated the role of the A1405G allele in anti-Ro±La seropositive pSS by considering epistasis with HLA DR3. The HLA region confers a strong genetic risk for seropositive pSS, and DRB1 alleles are markers of this risk. In Caucasian populations, the strongest association is observed with HLA DR3, although other DRB1 alleles are also involved, and the associated alleles are known to differ across different racial groups.
Epistasis between P2RX7 1405G and HLA DR3 in anti Ro±La seropositive primary Sjögren's syndrome (pSS)
Seropositive pSS (n= 95) versus controls (n= 136)
Seropositive pSS (n= 199) versus controls (n= 534)
Seropositive (n= 191) versus seronegative pSS (n= 80)
Seronegative pSS (n= 82) versus controls (n= 534)
Cohort 1: DRB1-typing
Cohort 2: DR3 proxy SNPa
Cohort 2: DR3 SSPa
Cohort 2: DR3 proxy SNPa
β0 (Intercept 1)
These analyses confirm that HLA DR3 is a strong risk factor for seropositive, but not seronegative pSS. However this association with DR3 appears somewhat greater in cohort 1 compared to cohort 2, which used a proxy SNP for DR3 status. For example, the regression coefficients (log-OR) reported in Table 3 equate to OR of 9.3 (95% CI 4.5, 19.2) for cohort 1, compared to 2.6 (95% CI 2.6, 5.8) for cohort 2. Further, there is remarkably consistent evidence of a statistically significant negative epistatic interaction between HLA DR3 (or proxy) and the P2RX7 1405G allele in seropositive pSS patients, whether comparing these patients to controls (in both cohorts), or seropositive pSS to seronegative pSS patients (sufficient data for cohort 2 only) (Table 3). This negative epistasis, which is assessed by β3 coefficient in Table 3, implies that the proportion of seropositive pSS patients who carry both alleles is significantly less than expected. However, the association between the P2RX7 1405G allele and seropositive pSS patients who do not carry DR3 (the β2 coefficient in Table 3) observed in cohort 1 (OR 3.0, 95% CI 1.4, 6.4) was not replicated in cohort 2 (OR 1.3, 95% CI 0.8, 2.0). This epistasis effect with DR3 was specific for the P2RX7 1405G allele, and was not observed with any other P2RX7 SNPs genotyped in cohort 1 (data not shown).
The most plausible interpretation of these findings is that the P2RX7 1405G allele is a risk factor for seropositive pSS in a specific subgroup of patients who do not carry HLA risk alleles, but confers no additional risk in individuals who do. In our analysis, the use of HLA DR3 would only partially adjust for the HLA risk, as other DRB1 alleles are also involved . Given that the OR for the association with DR3 are greater in cohort 1 than in cohort 2 (Table 3), this implies that other DRB1 alleles may be relatively more important in cohort 2, yet the analysis could not adjust for this. This difference in HLA risk adjustment between cohorts may be sufficient to explain the lack of replication of the P2RX7 1405G allele association in cohort 2 (Table 3).
The 1405G allele is a tag for a common P2RX7haplotype
The 1405G allele is associated with gain-of-function of the P2X7 receptor
Serum IL-18 levels are increased in pSS patients with Ro/La autoantibodies
Autoantibodies, which target intracellular antigens, are a feature of systemic autoimmune diseases such as pSS. These autoantibodies react with autoantigen exposed on apoptotic or necrotic cells, or their debris, and resulting immune complexes promote chronic systemic inflammation via upregulation of the type I interferon system . Delayed or defective clearance of apoptotic/necrotic cellular debris is understood to be an important factor in the breakdown of self-tolerance and ongoing immune complex mediated inflammation . Ro±La autoantibodies are typically present in the majority of pSS patients (70 to 82% in the present study) and are associated with higher levels of glandular inflammation, and extraglandular features such as vasculitis, interstitial lung disease and lymphoma . There is increasing evidence that autoantibody-positive pSS has different genetic risk factors to autoantibody-negative pSS [3, 4], which possibly reflects different mechanisms of disease pathogenesis.
In this study we examined the interaction of the pro-inflammatory P2X7 receptor in the pathogenesis of Ro±La autoantibody-positive pSS (seropositive pSS) in two cohorts. The primary focus was on the P2RX7 1405G allele, which is a tag for a common haplotype conferring gain-of-function on the P2X7 receptor [30, 31]. This haplotype also carries minor alleles from two other SNPS, 489T and 1068A (Table 1, Figure 1), and analysis of the functional effects of each in isolation suggests that they contribute, in an additive way, to the increased receptor function observed with this haplotype [30, 43]. We have demonstrated an interaction between this gain of function SNP/haplotype and seropositive pSS, whereas no associations were observed with loss of function SNPs/haplotypes. Previous studies of P2RX7 polymorphism and systemic autoimmune disease have reported no association between the A1513C loss-of-function SNP in Caucasian patients with SLE or RA [44, 45], which is consistent with the results of the present study.
We observed a remarkably consistent negative epistatic interaction between P2RX7 1405G and HLA DR3, the primary genetic risk factor for pSS, in two cohorts of seropositive pSS patients. This negative epistatic interaction was present when seropositive pSS patients were either compared to controls, or to seronegative pSS patients, so that the combined risk for individuals who carry both genetic factors is somewhat less than expected. In cohort 1 we also observed that the P2RX7 1405G allele is a risk factor for seropositive pSS in individuals who do not carry HLA DR3, but this effect was not replicated in cohort 2. The analysis in cohort 2 included principal components (derived from unrelated genetic markers) as covariates to adjust for possible population stratification, which can cause spurious associations in disease studies . Therefore, the observed epistasis cannot be attributed to systematic ancestry differences between cases and controls.
The most plausible interpretation of these findings is that the P2RX7 1405G allele is a risk factor for seropositive pSS in a specific subgroup of patients who do not carry HLA risk alleles, but confers no additional risk in individuals who do, and that differences in results between the cohorts may reflect differences in adjustment for the HLA associated risk, which is only partially reflected by DR3 status. For example, it is unclear whether the use of a surrogate SNP for determining HLA-DR3 status in the cohort 2 controls may have influenced the results by underestimating the prevalence of HLA-DR3; further, HLA-DR2 is also associated with autoantibody-positive pSS , but this information was not available for cohort 2.
The epistatic interaction between DR3 and the P2RX7 1405G allele in seropositive pSS implies overlap in pathogenic mechanisms associated with these alleles. Unfortunately, the pathogenic mechanisms underlying HLA-associated disease susceptibility are not understood, therefore, the interpretation is highly speculative. We propose that the observed epistasis between DR3 and the P2RX7 1405G allele may reflect increased autoantigen exposure as a susceptibility mechanism for seropositive pSS.
Complement deficiency is implicated in the defective clearance of apoptotic/necrotic debris and, therefore, increased autoantigen exposure, in systemic autoimmune disease . It has been proposed that this mechanism may contribute to some of the pSS disease susceptibility associated with HLA DR3, which is in linkage disequilibrium with a C4 null allele . P2X7 receptor activation may also contribute to increased autoantigen exposure in systemic autoimmunity. In the presence of the ATP ligand, P2X7 receptor activation may result in the induction of cell membrane blebbing and release of microparticles. Prolonged activation of the P2X7 receptor by ATP results in cell death with both apoptotic/necrotic features [13, 14], which has also been demonstrated in rat parotid acinar ParC5 cells , accompanied by autoantigen cleavage . Importantly, the A1513C loss of function SNP is associated with a decrease in ATP-induced apoptosis in CD4(+) lymphocytes isolated from SLE patients , although the gain-of-function polymorphism has not yet been evaluated. Membrane microparticles released by the P2X7-induced blebbing process contain not only P2X7 receptors but also Ro-52, a component of the P2X7 membrane complex both in THP-1 macrophages and transfected HEK-293 epithelial cells , and also a target of autoantibodies in pSS. Further, we have recently demonstrated that P2X7, in the absence of ATP, acts as a scavenging receptor for apoptotic cellular debris , although the downstream effects of this have not been elucidated.
In addition to the genetic epistasis, our data suggest that there is P2X7 receptor activation in seropositive pSS. Activation of P2X7 is known to lead to processing and secretion of pro-inflammatory cytokines IL-1β and IL-18 from monocyte/macrophages via activation of the NALP3 inflammasome, which is thought to play a role in a spectrum of inflammatory diseases , including rheumatic inflammatory diseases . We have demonstrated elevated serum IL-18 levels in Ro±La autoantibody-seropositive pSS patients, and activation of the NALP3 inflammasome, via P2X7, may be one of the mechanisms involved. There was no evidence for an effect of DR3 or 1405G status on IL-18 levels, although patient numbers were limited. Previous studies in systemic inflammatory diseases have demonstrated that the ATP-induced monocyte P2X7 receptor response (as measured by IL-1β secretion) is attenuated in SLE, but enhanced in RA [45, 53], and that this response is influenced by P2RX7 polymorphism. However, the A1405G SNP has not been evaluated in this context.
Aside from its pro-inflammatory effects, ATP activation of the P2X7 receptor has also emerged as an important regulator of autonomic function, including salivary gland secretion. While P2X7 receptor activation can stimulate secretion in mouse submandibular bands, activation also results in the inhibition of muscarinic receptor-induced fluid secretion . Therefore, activation of P2X7 in the milieu of salivary gland inflammation in pSS may contribute to the failure of fluid secretion irrespective of the extent of glandular destruction, through interference of autonomic signalling, a well-documented phenomenon in pSS. This dysregulation of autonomic function may also be implicated in the clustering of autonomic symptoms we have previously reported in pSS , and further research into the role of P2X7 and autonomic dysfunction in pSS is warranted.
In summary, we have identified that the P2RX7 1405G gain-of-function allele exhibits a negative epistatic interaction with HLA DR3 on the risk of autoantibody-positive pSS. This, coupled with other studies, implicates P2X7 receptor function in the pathogenesis of primary Sjogren's syndrome.
enzyme-linked immunosorbent assay
Oklahoma Medical Research Foundation
primary Sjögren's syndrome
systemic lupus erythematosus
single nucleotide polymorphism.
The authors gratefully acknowledge the support of the patients who participated in this study.
This study was funded by support from Arthritis Australia, National Health and Medical Research Council (NHMRC, grant number 509184), and The Queen Elizabeth Hospital Research Foundation
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