Interferon-gamma inducible protein-10 as a potential biomarker in localized scleroderma
© Magee et al.; licensee BioMed Central Ltd. 2013
Received: 5 September 2013
Accepted: 7 November 2013
Published: 13 November 2013
The purpose of this study was to evaluate the presence and levels of interferon-gamma inducible protein-10 (IP-10) in the plasma and skin of pediatric localized scleroderma (LS) patients compared to those of healthy pediatric controls and to determine if IP-10 levels correlate to clinical disease activity measures.
The presence of IP-10 in the plasma was analyzed using a Luminex panel in 69 pediatric patients with LS and compared to 71 healthy pediatric controls. Of these patients, five had available skin biopsy specimens with concurrent clinical and serological data during the active disease phase, which were used to analyze the presence and location of IP-10 in the skin by immunohistochemistry (IHC).
IP-10 levels were significantly elevated in the plasma of LS patients compared to that of healthy controls and correlated to clinical disease activity measures in LS. Immunohistochemistry staining of IP-10 was present in the dermal infiltrate of LS patients and was similar to that found in psoriasis skin specimens, the positive disease control.
Elevation of IP-10 levels in the plasma compared to those of healthy controls and the presence of IP-10 staining in the affected skin of LS patients indicates that IP-10 is a potential biomarker in LS. Furthermore, significant elevation of IP-10 in LS patients with active versus inactive disease and correlations between IP-10 levels and standardized disease outcome measures of activity in LS strongly suggest that IP-10 may be a biomarker for disease activity in LS.
Scleroderma, a connective tissue disease characterized by cutaneous sclerosis, is a broad term that encompasses both forms of the disease: systemic sclerosis (SSc) and localized scleroderma (LS), also known as morphea. SSc is a systemic disorder characterized by skin, vascular and visceral organ sclerosis, which more commonly affects adults. LS, which is more prevalent in children, is characterized by sclerosis that is typically limited to the skin, subcutis, and underlying bone and tissue without vascular or internal organ involvement. Although both SSc and LS share a common underlying pathophysiology of excessive production and deposition of collagen and sclerosis in an autoimmune setting, they are clinically different with unique morbidities and prognoses. LS has a different pattern of morphology and skin lesion distribution than SSc; it encompasses several subtypes including plaque morphea (circumscribed superficial), generalized morphea, linear scleroderma of the trunk/extremities or head, deep morphea (circumscribed deep), pansclerotic morphea and mixed morphea .
Though the dermatopathology of these entities is similar and sometimes difficult to differentiate, there are a few characteristics which dermatopathologists document as occurring more frequently in LS compared to SSc, such as more overlying epidermal atrophy, more intense inflammation and more diffuse dermal sclerosis . Both LS and SSc share findings of an earlier active disease phase with newer lesions demonstrating a lymphocytic infiltrate with a variable number of plasma cells and eosinophils . As lesions evolve, inflammation density decreases as collagen bundles thicken and skin sclerosis increases in the later fibrotic phase of the disease .
The inflammatory pathway of scleroderma has been associated with several cytokines and chemokines. In addition to playing a role in the physiological process of immune cell maturation and trafficking, chemokines induce, maintain and amplify inflammatory and immune reactions . Previous reports suggest that cytokines of various T-helper cell lineages contribute to both forms of scleroderma, LS and SSc [4, 5]. An inflammatory chemokine of interest in this study, interferon-gamma inducible protein-10 (IP-10, CXCL10), belongs to the CXC chemokine subfamily and is known to play a role in inflammatory responses in several autoimmune diseases, including systemic lupus erythematosus , juvenile dermatomyositis  and SSc [5, 8]. IP-10 acts through CXCR3 receptors that attract Th-1-type lymphocytes to inflammatory sites in the skin and contributes to several skin diseases, including psoriasis . Interferon-gamma (IFN-γ) stimulates the secretion of IP-10 from keratinocytes and other immune cells, including leukocytes, neutrophils, eosinophils, macrophages and monocytes, which induces an inflammatory response . Activated Th cells, B cells, macrophages and NK cells express CXCR3 and are then attracted to the inflamed tissue areas by IP-10 , which may account for the inflammatory infiltrate present in the skin of scleroderma patients.
In regards to scleroderma, most studies to date have focused on IP-10 expression in SSc. IP-10 expression is elevated in the serum , plasma  and skin  of SSc patients compared to that of healthy controls, and levels reflect active disease [5, 8]. Furthermore, recent studies in SSc have shown that elevated plasma IP-10 levels correlate significantly with the Medsger Severity Index for muscle, skin and lung involvement and thus act as a serological marker of disease severity . However, the expression of IP-10 in the peripheral circulation and skin of LS patients has not been studied. Thus, this study was designed to evaluate IP-10 in the circulation and local tissue in LS patients, with the additional goal of comparing IP-10 to standardized disease activity parameters.
The University of Pittsburgh Institutional Review Board (IRB) approved four separate protocols for (1) blood sample and clinical data collection of LS patients, (2) blood sample collection from de-identified healthy controls, (3) skin sample analysis of LS patients and (4) skin sample analysis of de-identified controls. Blood sample and clinical data collection of LS patients was performed through the National Registry of Childhood Onset Scleroderma (NRCOS) which has been approved by the University of Pittsburgh IRB since 2003. Consent was obtained from parents for their children’s participation in the NRCOS study and assent was obtained when appropriate. Healthy plasma control samples were obtained through an IRB approved protocol to collect and store discarded ‘waste’ samples resulting from well child visits at the University of Pittsburgh. The study team was not involved in this sample collection. After making a request to this protocol, an honest broker provided the study team with de-identified samples. Only basic demographic information associated with the healthy plasma was given to the study team, including age and sex.
For skin sample analysis, waivers of informed consent were granted by the IRB for two separate protocols; one to access retrospective LS patient skin specimens and one to access healthy and disease control samples. The IRB approved the waivers of informed consent after determining the protocols were minimal risk and the waivers did not adversely affect the rights and welfare of the subjects. Samples from LS patients were collected as part of clinical care, stored in the dermatopathology laboratory and accessed retrospectively by the study team. An honest broker was used to procure the control skin samples from the dermatopathology laboratory, which were given to the study team in a de-identified manner.
The study of IP-10 in the plasma of LS subjects was performed first and included subjects with both active and inactive disease states. Subjects from this study who had available skin biopsy specimens with concurrent clinical and serological assessment during the active phase of disease were included in the immunohistochemisty IP-10 staining study.
Blood specimen collection and clinical data
All LS patients had clinic visits from 2003 to 2012, during which standardized demographic, clinical and laboratory data were collected. Research blood samples were obtained from subjects by venipuncture at routine clinical visits as part of their participation in the pediatric scleroderma registry at the Children’s Hospital of Pittsburgh. Plasma was separated from whole blood via density-gradient centrifugation at 4°C within four hours of blood collection and promptly stored in 200 μL aliquots at -80°C until experimentation.
At the concurrent visit in which plasma was obtained, standardized clinical LS outcome measures were collected, including two disease activity measures: physician global assessment of activity (PGA-A) and the modified Localized Scleroderma Skin Score (mLoSSI) . The PGA-A is usually completed in conjunction with the mLoSSI, and both outcome measures were used to determine if the patient’s disease status at the time of sample collection was ‘active’ or ‘inactive’. The PGA-A is graded on a 100-mm analog scale and includes the following cutaneous variables: new lesions within the previous month, enlargement of existing lesion within the previous month, erythema/violaceous color at the border of lesion and skin thickening/induration at the border of lesion . The mLoSSI includes the sum of three separate scores from the following domains: erythema (none, mild, moderate, severe), skin thickness (none, mild, moderate, severe) and new lesion/lesion extension (present, not present) . A patient was classified as having active disease if they had a PGA-A greater than zero and a mLoSSI greater than zero. The only available demographic information obtained for the de-identified healthy plasma controls was their age and gender. These samples were obtained at well child evaluations at the University of Pittsburgh, and, therefore, should represent a similar population.
Plasma IP-10 levels (detection limit: 7.5 pg/ml to 10,000 pg/ml) were measured using the Luminex bead immunoassay system (BioRad, Hercules, CA, USA), according to the manufacturer’s instructions at the University of Pittsburgh Cancer Institute (UPCI) Luminex Core Facility. The plasma samples were undiluted and compared to a High PMT-Standard Dilution Series (BioRad, Hercules, CA, USA). To investigate the reliability of the samples included in the Luminex analysis, levels of IP-10 in the plasma of subjects (LS and healthy) were measured in duplicate. Inter-panel and intra-assay control plasma samples were included to ensure consistency across panels.
Tissue specimen collection
All skin specimens were accessed retrospectively by the study team per approved IRB protocols. The LS subjects’ skin specimens of interest included those with plasma IP-10 analysis during the active state of disease (typically their first clinical visit to the scleroderma center) with concurrent clinical assessment performed within a few months of the biopsy. Both the biopsy and plasma specimens of these subjects (n = 5) were obtained before any systemic therapy was initiated. At our center, typically the dermatologists will obtain a skin biopsy to confirm the diagnosis and then quickly refer to the pediatric rheumatology service in Pittsburgh for further management. The skin specimens from pediatric LS, psoriasis and healthy subjects consisted of residual tissues from 4 mm punch biopsies obtained for clinical purposes. All of the skin biopsies were performed by dermatologists at the University of Pittsburgh Medical Center, not by the patient’s treating rheumatologist or member of the study team. Samples were processed and stored according to a standard clinical protocol with immediate immersion in formalin for 24 to 48 hours, followed by fixation in paraffin for long term storage. Once requested by the study team, the paraffin blocks were made accessible for immunohistochemistry (IHC) analysis.
Immediately prior to experimentation, the paraffinized block specimens were sectioned into 6 μm sections and fixed onto glass microscopy slides. IHC was performed on serial skin biopsy sections. Tissue was de-paraffinized in xylene and rehydrated sequentially in ethanol. Antigen retrieval was carried out using a steamer and endogenous peroxidases were quenched to reduce non-specific background staining. The tissue was blocked using 5% goat serum 4% BSA blocking buffer according to standard protocol. The tissue was then analyzed for the presence and location of IP-10 staining. To decrease variability, during the analysis of each individual LS patient, a psoriasis disease control and healthy control specimen were studied in tandem. Psoriasis was chosen as a positive disease control because IP-10 staining has been demonstrated in the dermis, as has been shown by Giustizieri et al. .
For each subject, a section of tissue was incubated overnight in the primary antibody, polyclonal IP-10 (1:250; Amgen Inc., Thousand Oaks, CA, USA) and the second section of the corresponding tissue was incubated overnight in the rabbit immunoglobulin G (IgG) isotype control (1:1000; Vector Laboratories, Inc., Burlingame, CA, USA) to serve as an antibody control. Following the primary antibody incubation, all tissue sections were washed in PBS and incubated with secondary antibody, biotinylated anti-rabbit IgG (1:200; Vector), for 30 minutes. The tissue was washed in PBS and incubated in Avidin Biotin Complex (ABC) (Vector) and aminoethylcarbazole solution (AEC) (Life Technologies Corporation, Carlsbad, CA, USA) for detection purposes. Images were acquired using an Olympus Provis microscope (Olympus America Inc., Center Valley, PA, USA).
Hematoxylin and eosin staining
To assess the presence and degree of cellular infiltration in the skin sections, hematoxylin and eosin (H & E) staining was carried out according to standard protocol in skin sections adjacent to those used for IHC.
All analyses were performed using SPSS v. 20 (SPSS, Chicago, IL. USA). Mean or median was used to describe data where appropriate. To determine if variability was present in the Luminex results between duplicate samples, intra-class correlation coefficients (ICC) were calculated. ICC values were interpreted according to the following ranges: excellent reproducibility: 0.6 to 1.0, moderate reproducibility: 0.4 to 0.59, and poor reproducibility: <0.4 .
Non parametric analyses were employed to evaluate differences in IP-10 plasma levels between subject groups using Wilcoxon rank sum analyses (α level of 0.05). IP-10 plasma levels were compared between LS and healthy controls, as well as within LS between active and inactive disease (activity classification based on clinical parameters). The relationship of cytokines to clinical measures of disease activity (PGA-A and mLoSSI) were also examined using Spearman’s rho (nonparametric correlation). Correlations of 0.16 to 0.29 were considered weak to low, 0.3 to 0.59 moderate to low, 0.5 to 0.69 moderate, 0.7 to 0.89 strong, and 0.9 to 1 very strong. Finally, IP-10 levels within classification subgroups of LS were compared to determine any differences. Subgroup classification was based on the Padua criteria .
The tissue was analyzed for the presence and location of IP-10 staining. To evaluate the general degree of inflammatory infiltrate in the tissue sectioned, a dermatopathologist (JH) also evaluated the H & E slides which corresponded to the IP-10 stained tissue per subject. He described the cellular infiltrate present and scored the degree of cellular infiltrate in three random high power fields (HPF) per LS tissue section to determine the average amount of inflammation per specimen (mild, moderate, severe). The degree of infiltration in each HPF was quantified according to the following scale: mild: 1 to 50 cells, moderate: 51 to 150 cells, severe: >150 cells.
Plasma analysis of IP-10
Demographic and baseline characteristics of 69 localized scleroderma (LS) subjects and 71 healthy control subjects who were included in the plasma analysis
(number = 69)
(number = 71)
Age at plasma sample in years
12.5 (10.0 to 16.0)
3 (2.0 to 9.0)
Age at disease onset in years
9 (7.0 to 11.0)
Linear scleroderma trunk/extremity
Linear scleroderma head (En coup de sabre / Parry Romberg Syndrome)
Plaque (circumscribed superficial) morphea
Deep (circumscribed deep) morphea
Clinical measures of activity:
41.0 (29.0 to 62.0)
6.0 (3.0 to 13.0)
IP-10 levels were significantly higher in active LS patients (median = 2,087.3 pg/ml, IQR = 956.6 to 3,099.0) compared to inactive LS patients (median = 880.5 pg/ml, IQR = 447.8 to 1,289.8, P = 0.001) (Figure 1). IP-10 levels in the plasma of LS patients had moderate to low, but statistically significant, correlations with scores of both clinical disease activity measures, the PGA-A (r s = 0.45, P <0.001) and mLoSSI (r s = 0.34, P = 0.004).
Tissue analysis of IP-10
Demographics, clinical activity measures and IP-10 analysis of localized scleroderma (LS) patients and controls (psoriasis and healthy) participating in tissue analysis
Clinical and laboratory features of tissue analyses subjects
(number = 5)
(number = 2)
(number = 2)
Plaque (circumscribed superficial)
Ages in years
Ages in years
Age at onset, years
13 (6 to 16)
Age at biopsy, years
13 (10 to 16)
IP-10 staining present in cellular infiltrate
Degree of cellular infiltratea
Mild (1 to 50)
Moderate (51 to 150)
Clinical measures and plasma IP-10 of LS patientsb
48 (41 to 52)
7 (7 to 8)
Plasma level of IP-10 (pg/ml)c
2,814 (745 to 3,081)
This study demonstrated IP-10 to be significantly elevated in the peripheral circulation of pediatric patients with LS in comparison to healthy controls. This corresponds to findings in adult SSc studies in both Caucasian [10, 14] and Japanese cohorts [5, 15]. In addition, IP-10 was also present in the local tissue directly affected by LS. Finally, we found significant positive correlations between validated and prospectively collected disease activity parameters (mLoSSI and PGA-A) and plasma IP-10 levels, supporting a potential role of IP-10 in the active disease phase of LS. This is the first time IP-10 levels have been demonstrated to correspond to disease activity measures in LS. In adults, SSc studies have also demonstrated IP-10 in the plasma to correlate with disease activity and severity parameters, including skin thickness .
A greater understanding of the origin of IP-10 in skin sclerosis is required to develop effective therapies for LS. An increased expression of IFN-inducible chemokines (IP-10) is most likely a response to the high IFN-γ skin levels in early stages of disease . IFN gene expression studies in SSc have shown that SSc patients have higher serum  and plasma IFN-inducible chemokine levels that correlate with microarray and qPCR IFN gene expression scores when compared with those of healthy controls .
In many Th-1-dominant autoimmune disorders, it has previously been determined that IP-10 and its receptor, CXCR3, play a role both in leukocyte recruitment to inflamed tissue and in the process of tissue damage . Both LS and SSc are characterized by a predominant lymphocytic infiltrate during the active phase of the disease. The exact lymphocyte cell type has not yet been identified in LS, but studies in SSc demonstrate a mixture of CD8+ and CD4+ cells in affected skin . It is possible that Th-1 cells, a subset of CD4+ T-helper cells, may be recruited locally to the site of the skin by IP-10. IP-10 belongs to the CXC chemokine family, known lymphocyte attractants, predominantly in areas of inflamed tissue . In LS, the active phase, which has previously been shown to be induced by T cell-derived cytokines, is followed by a damage phase characterized by sclerosis . The result of sclerosis and atrophy of the skin and underlying tissues may cause significant deformity and severe functional impairment . Thus, it is essential to target potential pathogenic inflammatory proteins, such as IP-10, in the active inflammatory phase of LS to minimize cumulative, permanent disease damage.
To our knowledge, this is the first study which provides evidence of IP-10 as a possible biomarker for LS. However, this study is limited due to its cross sectional nature and small sample size, specifically for the results pertaining to skin samples. Also, the healthy control plasma samples were as a whole, younger than the LS patients. It would be interesting to evaluate if these results were replicable in a group of LS patients with age matched controls. A longitudinal approach of the peripheral blood IP-10 expression as disease status changes with systemic therapy is currently being undertaken by our study team and would assist in further defining IP-10’s role as a disease activity biomarker or predictor. Our ultimate goal in the investigation of the biologic contribution of IP-10 and the IFN pathway to disease activity will hopefully aid in the development of more directed and efficacious therapies.
Our study indicates IP-10 as a potential clinically actionable biomarker in localized scleroderma by reflecting active disease, which may be useful for clinical management, such as medication response. Future directions include longitudinal analysis of peripheral IP-10 levels as the disease transitions from active to inactive states and later flares.
Bovine serum albumin
Children’s Hospital of Pittsburgh of the University of Pittsburgh Medical Center
- H & E:
Hematoxylin and eosin
High power fields
Intra-class correlation coefficient
Interferon-gamma inducible protein-10
Institutional review board
Modified localized scleroderma skin score
National registry of childhood onset scleroderma
Polymerase chain reaction
Physician global assessment of activity
University of Pittsburgh Cancer Institute.
This study was made possible by funding through the Nancy Taylor Foundation for Chronic Diseases Inc. (KT), the NIAMS Mentored Patient Oriented Research Award, NIH Grant No. K23 AR059722 (KT), and the NIAMS Mid-Career Investigator Award in Patient-Oriented Research, NIH Grant No. K24 AR060297 (CFB). We appreciate their continued support.
We would like to thank the Research Histology Services at the University of Pittsburgh for their assistance with skin biopsy processing and hematoxylin and eosin (H & E) staining. We would also like to thank the Luminex Core Facility Services at the University of Pittsburgh Cancer Institute (UPCI) for their assistance with plasma analysis and Jason Devlin at the Center for Biologic Imaging at the University of Pittsburgh for his assistance with microscopy. We would like to thank Abbe de Vallejo, PhD for his assistance with the collection of healthy plasma samples. We would like to thank Thaschawee Arkachaisri, MD for establishing the pediatric scleroderma clinic and registry at the University of Pittsburgh. Lastly, we would like to thank Cody Caplinger for his assistance with organizing and maintaining the Pittsburgh Localized Scleroderma Database and Thomas Medsger Jr., MD for his continued mentoring and support. Written informed consent was obtained from the parents of LS patients for publication of this manuscript. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
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