Volume 4 Supplement 1

22nd European Workshop for Rheumatology Research

Open Access

Electrotransfer of low doses of plasmid encoding interleukin-10 in gene therapy of collagen-induced arthritis

  • Mc Boissier1
Arthritis Research & Therapy20024(Suppl 1):111

DOI: 10.1186/ar447

Received: 15 January 2002

Published: 4 February 2002

Gene therapy is extremely promising in rheumatoid arthritis (RA). Electrotransfer (ET) is a recent method reported to enhance the in vivo effects of intra-muscular DNA injection. Interleukin-10 (IL-10) has anti-inflammatory effects in RA and in collagen-induced arthritis (CIA), a murine model of RA. We used ET to enhance penetration into skeletal muscle of plasmids encoding IL-10. CIA was induced in DBA/1 mice by immunization with bovine type II collagen. Injection into the tibial cranial muscle of low-dose (200 ng) pCOR plasmid encoding murine IL-10 (pCOR-CMV-mIL-10) was immediately followed by application of square-wave electric pulses (8 pulses of 200 V/cm, 20 ms duration at 2 Hz). Control groups received empty plasmid or saline before ET. When ET was performed twice on days 10 and 25 post-immunization, CIA was significantly delayed (P < 0.05) and attenuated (P < 0.001) in pCOR-CMV-mIL-10 ET groups vs. control groups. When pCOR-CMV-mIL-10 ET was started at disease onset (days 25 and 40), the clinical severity of CIA was reduced (P < 0.05). All groups treated early or late by pCOR-CMV-mIL-10 ET showed dramatic suppression of histological signs of arthritis compared to control groups. Taken together, these data indicate that administration of an anti-inflammatory gene by ET of naked DNA is effective in vivo in an arthritis model in preventive and curative protocols, even when low doses were given.

Authors’ Affiliations

(1)
Hôpital Avicenne

Copyright

© BioMed Central Ltd 2002

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