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Table 1 Species of fibronectin bearing the N-terminal heparin-binding domain in samples of synovial fluid from patients with osteoarthritis (OA) and rheumatoid arthritis (RA)a

From: Electrophoretic characterization of species of fibronectin bearing sequences from the N-terminal heparin-binding domain in synovial fluid samples from patients with osteoarthritis and rheumatoid arthritis

 

Fibronectin species bearing N-terminal heparin-binding domain

Synovial fluid sample

~200+ kDa pI ~4.9–5.9

~200+ kDa pI ~4.0–4.4

~170 kDa pI ~6.0–7.0

~170 kDa pI ~4.3

~185 kDa pI ~5.8–6.4

~180 kDa pI ~5.5–5.8

~130 kDa pI ~9.1

From OA

       

1

+

-

+

-

+

+

NT

2

+

+

-

-

-

-

NT

3

+

-

+

-

+

+

+

4

+

+

+

+

-

+

NT

5

+

+

+

-

+

-

+

6

+

-

+

-

-

-

NT

7

+

-

+

-

-

+

NT

8

+

-

+

-

+

+

+

9

+

+

+

+

-

-

+

+/totalb

9/9

4/9

8/9

2/9

4/9

5/9

4/4c

From RA

       

10

+

-

+

-

+

-

NT

11

+

+

+

-

+

+

NT

12

+

+

+

-

-

+

NT

13

+

+

+

-

+

+

NT

14

+

-

+

-

+

+

NT

15

+

+

+

-

+

+

NT

16

+

+

-

-

-

-

NT

17

+

+

+

+

-

-

NT

18

+

+

+

-

+

-

NT

19

+

+

-

-

-

-

NT

+/totalb

10/10

8/10

8/10

1/10

6/10

5/10

NT

  1. aSamples of synovial fluid were subjected to two-dimensional electrophoresis in linear pH 4–7 isoelectric focusing gradients followed by reduced 5% SDS–PAGE and Western blot analysis using mAb 1936 specific for the N-terminal heparin-binding domain of fibronectin. OA samples 3, 5, 8 and 9 were additionally subjected to analysis using pH 3–10 linear first-dimension gradients, which permitted detection of a ~130-kDa N-terminal species (rightmost column). bThe numerator is the number of samples in which a particular species of FN was detected (+) and the denominator is the total number of samples tested. cFour OA samples and no RA samples were subjected to pH 3–10 gradients, which permitted detection of the ~130-kDa species. -, species not detected; NT, not tested.