Skip to main content

Table 1 Species of fibronectin bearing the N-terminal heparin-binding domain in samples of synovial fluid from patients with osteoarthritis (OA) and rheumatoid arthritis (RA)a

From: Electrophoretic characterization of species of fibronectin bearing sequences from the N-terminal heparin-binding domain in synovial fluid samples from patients with osteoarthritis and rheumatoid arthritis

  Fibronectin species bearing N-terminal heparin-binding domain
Synovial fluid sample ~200+ kDa pI ~4.9–5.9 ~200+ kDa pI ~4.0–4.4 ~170 kDa pI ~6.0–7.0 ~170 kDa pI ~4.3 ~185 kDa pI ~5.8–6.4 ~180 kDa pI ~5.5–5.8 ~130 kDa pI ~9.1
From OA        
1 + - + - + + NT
2 + + - - - - NT
3 + - + - + + +
4 + + + + - + NT
5 + + + - + - +
6 + - + - - - NT
7 + - + - - + NT
8 + - + - + + +
9 + + + + - - +
+/totalb 9/9 4/9 8/9 2/9 4/9 5/9 4/4c
From RA        
10 + - + - + - NT
11 + + + - + + NT
12 + + + - - + NT
13 + + + - + + NT
14 + - + - + + NT
15 + + + - + + NT
16 + + - - - - NT
17 + + + + - - NT
18 + + + - + - NT
19 + + - - - - NT
+/totalb 10/10 8/10 8/10 1/10 6/10 5/10 NT
  1. aSamples of synovial fluid were subjected to two-dimensional electrophoresis in linear pH 4–7 isoelectric focusing gradients followed by reduced 5% SDS–PAGE and Western blot analysis using mAb 1936 specific for the N-terminal heparin-binding domain of fibronectin. OA samples 3, 5, 8 and 9 were additionally subjected to analysis using pH 3–10 linear first-dimension gradients, which permitted detection of a ~130-kDa N-terminal species (rightmost column). bThe numerator is the number of samples in which a particular species of FN was detected (+) and the denominator is the total number of samples tested. cFour OA samples and no RA samples were subjected to pH 3–10 gradients, which permitted detection of the ~130-kDa species. -, species not detected; NT, not tested.