Figure 7From: Intramuscular electroporation with the pro-opiomelanocortin gene in rat adjuvant arthritisConfocal micrographs of POMC gene electroporation on endorphin expression in muscle. (A) Immunohistochemical detection of endorphin immunoreactivity in the negative control that omitted the primary antibody. A muscle nucleus is labelled by 4,6-diamidino-2-phenylindole (blue). (B) Overexpression of endorphin immunoreactivity (green) in the POMC-treated group. Scale bar, 40 μm. (C) High-magnification image (from (B)) of endorphin-positive puncta shows the detailed distribution of endorphin in a muscle cell. Scale bar, 20 μm. (D) Endorphin-positive puncta are absent from muscle electroporated with pCMV-Script vector alone. Scale bar, 20 μm. (E, F) No significant inflammatory cell infiltration or muscle damage on haematoxylin/eosin staining of POMC-electroporated muscle (E) and untreated muscle (F). Original magnification, ×200. Scale bar, 100 μm.Back to article page