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Figure 3 | Arthritis Res Ther

Figure 3

From: Decreased response to IL-12 and IL-18 of peripheral blood cells in rheumatoid arthritis

Figure 3

Effect of IL-12 and IL-18 on rheumatoid arthritis synovium cells. (a) Rheumatoid arthritis synovium cells (1 × 106/ml in RPMI 1640 medium with 10% fetal calf serum) were cultured in triplicate for 7 days with or without 1 ng/ml of IL-12, 5 ng/ml of IL-18, 2 μg/ml of IL-18 binding protein (IL-18BP), or a combination of these. (b) Cells were activated with 200 ng/ml of phytohemagglutinin (PHA) and 2 ng/ml of phorbol 12-myristate 13-acetate (PMA). IFN-γ concentrations in culture supernatants were measured by ELISA. *P < 0.05.

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