Figure 3
Lapine cartilage: modulation of stimulated and inhibited proteoglycan synthesis by p38 mitogen-activated protein kinase inhibition. Cartilage slices were maintained overnight in Dulbecco's modified Eagle's medium (DMEM)/10% fetal calf serum (FCS), the serum removed, and agonists and inhibitors added 24 hours later. Then 1 μM SB 202190 was added 60 min before 0.1 ng/ml IL-1, and FCS and transforming growth factor beta (TGF-β) were added 6 hours later. Proteoglycan synthesis was evaluated the following day as the amount of 35S incorporated into proteoglycans expressed as pmol/10 mg wet weight. Values are the mean ± standard error of n = 6–12. *P < 0.05 versus vehicle.