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Figure 2 | Arthritis Res Ther

Figure 2

From: Does protein kinase R mediate TNF-α- and ceramide-induced increases in expression and activation of matrix metalloproteinases in articular cartilage by a novel mechanism?

Figure 2

Quantitative analysis of (a) MMP-2 and (b)MMP-9 synthesis by bovine articular cartilage. Medium was collected 24 hours after treatment of explants with 100 ng/ml TNF-α or 50 μM C2-ceramide in the presence or absence of 2-AP (10 mM) and analysed by gelatin substrate zymography. The area (absorbance units) of substrate gel cleared by proMMP-9 and active MMP-9 was measured by scanning densitometry. Each area obtained was related to the gel clearance obtained with the fibroblast-conditioned-medium standard in order to facilitate comparisons between gels. Data shown are arbitrary units per milligram of wet weight of tissue and are expressed as means ± SEM. *Significantly different from control explants at P < 0.05; **P < 0.01. 2-AP, 2-aminopurine; MMP, matrix metalloproteinase; TNF, tumour necrosis factor.

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Arthritis Research & Therapy

ISSN: 1478-6362

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