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Figure 5 | Arthritis Res Ther

Figure 5

From: Does protein kinase R mediate TNF-α- and ceramide-induced increases in expression and activation of matrix metalloproteinases in articular cartilage by a novel mechanism?

Figure 5

The PKR inhibitor 2-AP blocks TNF-α and C2-ceramide-induced proteoglycan synthesis. Cartilage explants were cultured for 24 hours in the presence of 2-AP (1 mM) alone, TNF-α (100 ng/ml) with or without 2-AP (1 mM) or C2-ceramide (100 μM) with or without 2-AP (1 mM). Explants were digested by papain (300 μg/ml) as described in Materials and methods and the amount of sGAG present determined as described above and expressed as micrograms glycosaminoglycan per milligram wet weight of cartilage. *Significantly different from control explants at P < 0.05; ** P < 0.01. 2-AP, 2-aminopurine; PKR, protein kinase R; sGAG, sulfated glycosaminoglycan; TNF, tumour necrosis factor.

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Arthritis Research & Therapy

ISSN: 1478-6362

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