Figure 2

Multiple alignment of partial peptidylarginine deiminase (PAD) protein sequences based on a large full alignment described in [4] (available online: http://www.mrw.interscience.wiley.com/suppmat/0265-9247/suppmat/2003/25/v25.1106.html). Shown are segments of all five isotypes from the human (Homo sapiens [Hs], PAD1 NP_037490, PAD2 NP_031391, PAD3 NP_057317, PAD4 NP_036519 and PAD6 XP_210118) and segments of PAD4 from the mouse (Mus musculus [Mm], NP_035191), the rat (Rattus norvegicus [Rn], NP_058923) and the cow (Bos taurus [Bt], based on BG364988). Conserved residues that are identical in more than 50% of all known PAD sequences are shaded black; fully conserved residues are shaded cyan. Conserved charged residues are also indicated (shaded light gray). Exon boundaries, based on PAD1 sequences, are annotated above the alignment. The monopartite nuclear localization signal (NLS) of PAD4 is shaded green, and conserved NLS residues are bold [8]. The four exonal single nucleotide polymorphisms are shaded pink. The nonsusceptible haplotype (S A A L) is shown in the alignment, and the susceptible (G V G L) haplotype is indicated below it. a.a., amino acid.