Figure 3

Time course of IP-10 transcription in MRL/lpr, MRL/+, and B6 mice. Whole RNA was isolated from mouse lung tissues at the indicated times (months); transcribed mRNA was amplified by RT-PCR. (a) Representative expression of IP-10 mRNA and Southern blot hybridization with internal probes; GAPDH primers were used as an internal control. Data are representative of three independent experiments. Lane M: molecular weight markers (100-base-pair ladder). (b) IP-10 transcripts were quantitated and normalized to GAPDH as IP-10/GAPDH transcripts ratio. Data are expressed as mean ± SEM of three independent experiments; *P < 0.05 vs individual age (4 months) of MRL/+ mice and B6 mice. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IP-10, interferon-γ-inducible protein 10; PCR, polymerase chain reaction; RT-PCR, reverse transcriptase PCR.