Figure 7

Time course of TARC and CCR4 transcription in the lungs of 1- and 4-month-old MRL/lpr, MRL/+, and B6 mice. Whole RNA was isolated from lung tissues, and transcribed mRNA was amplified by RT-PCR. (a) Representative expression of TARC and CCR4 mRNA; GAPDH primers were used as an internal control. Data are representative of three independent experiments. Lane M: molecular weight markers (100-base-pair ladder). (b) TARC and (c) CCR4 transcripts were quantitated and expressed relative to GAPDH as the TARC/GAPDH or CCR4/GAPDH transcripts ratio. Data are expressed as mean ± SEM of three independent experiments. (b) *P < 0.05 vs 4-month-old B6 mice; **P < 0.01 vs 1-month-old B6 mice. (c) *P < 0.05 vs 1-month-old MRL/+ mice and 4-month-old MRL/+ mice and B6 mice, **P < 0.01 vs 1-month-old B6 mice. CCR4, TARC receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-PCR, reverse transcriptase polymerase chain reaction; TARC, thymus- and activation-regulated chemokine.