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Table 3 Epitope mapping of giantin

From: Giantin is the major Golgi autoantigen in human anti-Golgi complex sera

Recombinant fragments

Total positive sera (n [%])

Low-positive sera (%)

High-positive sera (%)

P1, aa 39–1040 (1001 aa)

12 (30.0)

7/12 (58.3)

5/12 (41.7)

P2, aa 851–1450 (599 aa)

11 (27.5)

8/11 (72.7)

3/11 (27.3)

P3, aa 1435–2204 (769 aa)

11 (27.5)

7/11 (63.6)

4/11 (36.4)

P4, aa 2019–2568 (549 aa)

7 (17.5)

3/7 (42.9)

4/7 (47.1)

P5, aa 2550–2843 (293 aa)

15 (37.5)

15/15 (100)

0

P6, aa 2818–3259 (441 aa)

22 (55.0)

22/22 (100)

0

  1. A total of 40 antigiantin positive human sera were analyzed for reactivity in ELISA. Cutoff value for a positive reaction: the mean optical density (OD) of normal human sera +3 standard deviations (SDs). Low-positive: the OD between the mean of normal sera +3 SDs to +15 SDs. High-positive: OD greater than the mean of normal sera +15 SDs. aa, amino acids.