Inhibition of established collagen-induced arthritis with a tumour necrosis factor-α inhibitor expressed from a self-contained doxycycline regulated plasmid

Table 2 Cytokine release from draining lymph node cells of collagen-induced arthritis mice

	Cytokine release from DLN cells
Treatment	IFN-γ (ng/ml): 48 hour CII	IFN-γ (ng/ml): 48 hour ConA	IL-4 (pg/ml): 48 hour CII	IL-4 (pg/ml): 48 hour ConA
GTRTEmpty + doxycycline	0.41 ± 0.03	4.2 ± 1.1	11.9 ± 3.2	33.2 ± 8.8
GTRTT – doxycycline	0.66 ± 0.15	2.4 ± 0.8	6.4 ± 1.5	24.4 ± 6.7
GTRTT + doxycycline	0.49 ± 0.04	2.8 ± 0.9	9.0 ± 1.6	21.5 ± 2.4

Results are presented from at least six animals in each group. Cytokine release from draining lymph node (DLN) cells was induced by 48 hours of stimulation with CII or ConA and was measured by ELISA. CII, collagen type II.

ISSN: 1478-6362