Systemic delivery of Ad-mIL-4. Adenoviruses were injected into thetail vein 24 h after lipopolysaccharide treatment. (a) Arthritis index.Subsequently, severity of disease was assessed every other day using anestablished macroscopic scoring system ranging from 0 to 4: 0, normal; 1,detectable arthritis with erythma; 2, significant swelling and redness; 3,severe swelling and redness from joint to digit; and 4, maximal swelling withankylosis or necrosis. + The average macroscopic score was expressed as acumulative value for all paws, with a maximum possible score of 16 per mouse(n =10). *P <0.001;**P<0.0005. (b) Paw swelling. The thickness of each paw was alsoevaluated using a spring-load caliper. The paw swelling for each mouse wascalculated by adding the four thicknesses of the individual paws.*P <0.05; **P <0.001. (c) Number of arthritis paws. The total number of arthritic paws were determinedfor each experimental group (maximum 40 per group). *P<0.001.