TY - JOUR AU - Granet, Corinne AU - Maslinski, Wova AU - Miossec, Pierre PY - 2004 DA - 2004/02/26 TI - Increased AP-1 and NF-κB activation and recruitment with the combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocytes JO - Arthritis Res Ther SP - R190 VL - 6 IS - 3 AB - To determine the contribution of IL-1β, tumor necrosis factor alpha (TNF-α) and IL-17 to AP-1, NF-κB and Egr-1 activation in rheumatoid arthritis, the effect of the cytokines used alone or in combination was measured on TF expression in rheumatoid synoviocytes. Effects on mRNA expression were measured by RT-PCR and effects on nuclear translocation were measured by immunocytochemistry. To assess the functional consequences of cytokine induction, osteoprotegerin levels were measured in synoviocyte supernatants. SN - 1478-6354 UR - https://doi.org/10.1186/ar1159 DO - 10.1186/ar1159 ID - Granet2004 ER -