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Figure 1 | Arthritis Res Ther

Figure 1

From: Increased AP-1 and NF-κB activation and recruitment with the combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocytes

Figure 1

Quantification of the effects of individual cytokines and their combinations on TF nuclear translocation. After 12 hours of culture without FCS, rheumatoid arthritis synoviocytes (104 cells/cm2) were stimulated for 30 min with cytokines at the cited concentrations. Immunofluorescence staining was performed with anti-TF specific antibodies and was confirmed by Hoechst H33258 staining. Using an image analysis software, the mean green intensity of nuclear staining was quantified in 10 randomly selected cells. Results are expressed as the mean of three independent experiments. Results were analyzed using the Kruskall–Wallis test. ***P < 0.001, **P < 0.01, *P < 0.05 versus medium alone. See Fig. 2 for an example of an actual picture. TNF, tumor necrosis factor.

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Arthritis Research & Therapy

ISSN: 1478-6362

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