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Figure 3 | Arthritis Res Ther

Figure 3

From: Increased AP-1 and NF-κB activation and recruitment with the combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocytes

Figure 3

Effects of IL-1β, tumor necrosis factor alpha (TNF-α) and IL-17 used alone and in combination on c-jun mRNA expression. After 12 hours of culture without FCS, rheumatoid arthritis fibroblast-like synoviocytes were stimulated for 20 min with individual or combined cytokines at low concentrations: IL-1 (10 pg/ml), TNF-α (10 pg/ml) and IL-17 (50 ng/ml). Cells were lyzed and total mRNA was extracted. RT-PCR was performed as described in Materials and methods. The intensity of the respective β-actin and c-jun bands was measured by densitometry. Results were expressed as the ratio between c-jun values divided by those of the internal control β-actin and were the mean of three independent experiments. CT, control.

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