Figure 4From: Increased AP-1 and NF-κB activation and recruitment with the combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocytesDetermination of osteoprotegerin (OPG) levels in supernatants obtained from rheumatoid arthritis (RA) synoviocytes stimulated by proinflammatory cytokines alone or in combination. RA synoviocytes were cultured with cytokines at the cited concentrations. After 72 hours of culture, supernatants were collected and OPG levels were measured by ELISA. Results are expressed as the mean of three independent experiments. TNF, tumor necrosis factor.Back to article page