Figure 3

Flow cytometric analysis for receptor activator of NF-κB (RANK) on splenocytes of immunised interferon-γ receptor knock-out (IFN-γR KO) and wild-type mice. Splenocytes were isolated on day 21 after immunisation. Cells were incubated with phycoerythrin-labelled anti-CD11b antibody, biotinylated anti-RANK antibody and fluorescein isothiocyanate-labelled streptavidin. The grey line represents staining with an irrelevant biotinylated IgG. RANK expression was analysed both within the CD11b^- (left panels) and within the CD11b⁺ (right panels) splenocyte population. The black line represents staining with anti-RANK–biotin/streptavidin–phycoerythrin. The total numbers of RANK⁺ cells within the CD11b⁺ population of the IFN-γR KO (upper right panel) and of the wild-type (lower right panel) splenocytes are indicated, together with the percentage that they represent of the total splenocyte population (each picture is representative for one mouse out of three).