CD14 mediates peptidoglycan–polysaccharide complex (PG-PS)-induced secretion of IL-6 and tumor necrosis factor (TNF)-α. Peritoneal macrophages were stimulated for 4 hours with the indicated concentrations of PG-PS. Concentrations of TNF-α and IL-6 were determined in supernatants by ELISA. Each determination was done in duplicates. (a) TNF-α production by peritoneal macrophages. Data represent means ± standard error of the mean from three independent experiments. (b) IL-6 production by peritoneal macrophages. Data represent mean ± SEM from two independent experiments.