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Table 1 PCR primers and Southern blot probes

From: Patients with systemic lupus erythematosus have abnormally elevated Epstein–Barr virus load in blood

Gene

Primers and probes

Sequence (5'-3')

Expected product size

PCR conditions

EBNA-3C

Forward primer

AGAAGGGGAGCGTGTGTTGT

Type 1: 153 bp

Type 2: 246 bp

94°, 30 s

61°, 60 s

72°, 60 s

 

Reverse primer

GGCTCGTTTTTGACGTCGGC

  
 

Probe

TCATAGAGGTGATTGATGTT

  

EBNA-2

Forward primer

AGGCTGCCCACCCTGAGGAT

Type 1: 168 bp

Type 2: 184 bp

94°, 30 s

64°, 45 s

72°, 30 s

 

Reverse primer

GCCACCTGGCAGCCCTAAAG

  

EBNA-3B

Forward primer

CCCTTGCGGATGCAGCCAAT

Type 1: 125 bp

Type 2: 149 bp

94°, 30 s

62°, 60 s

72°, 60 s

 

Reverse primer

GGCTGATATGGAATGTGCCC

  
  1. EBNA, Epstein–Barr virus nuclear antigen.