From: Patients with systemic lupus erythematosus have abnormally elevated Epstein–Barr virus load in blood
Gene | Primers and probes | Sequence (5'-3') | Expected product size | PCR conditions |
---|---|---|---|---|
EBNA-3C | Forward primer | AGAAGGGGAGCGTGTGTTGT | Type 1: 153 bp Type 2: 246 bp | 94°, 30 s 61°, 60 s 72°, 60 s |
Reverse primer | GGCTCGTTTTTGACGTCGGC | |||
Probe | TCATAGAGGTGATTGATGTT | |||
EBNA-2 | Forward primer | AGGCTGCCCACCCTGAGGAT | Type 1: 168 bp Type 2: 184 bp | 94°, 30 s 64°, 45 s 72°, 30 s |
Reverse primer | GCCACCTGGCAGCCCTAAAG | |||
EBNA-3B | Forward primer | CCCTTGCGGATGCAGCCAAT | Type 1: 125 bp Type 2: 149 bp | 94°, 30 s 62°, 60 s 72°, 60 s |
Reverse primer | GGCTGATATGGAATGTGCCC |