CD25brightCD4+regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease

Table 2 Cytokine production and proliferation of CD25^- CD4⁺ T cells in in vitro cultures

Patient	IFN-γ(pg/ml)	IL-10 (pg/ml)	IL-13 (pg/ml)	TNF (pg/ml)	Proliferation (c.p.m.)
PsA 1^a	6656	459	952	na	67,331
PsA 2^b	4775	67	na	468	42,662
SpA 1^a	879	bdl	bdl	na	13,086
SpA 2^b	3681	bdl	na	806	42,469
JIA 1^b	1924	53	na	355	105,210
JIA 2^b	1656	30	na	530	12,621
RA 1^a	5677	385	293	na	106,269
RA 2^b	608	bdl	na	109	103,724

Production of cytokines and proliferation of responder cells in patients with psoriatic arthritis (PsA), spondyloarthopathies (SpA), juvenile idiopathic arthritis (JIA) and rheumatoid arthritis (RA) are shown. Changes in cytokine and proliferation levels after coculture with CD25^brightCD4⁺ T cells are shown in Fig. 5. bdl, below detection limit; IFN, interferon; IL, interleukin; na, not analyzed; TNF, tumor necrosis factor. ^aEnzyme-linked immunosorbent assay; detection limit is 50 pg/ml; 20,000 cells per well in coculture. ^bCytometric bead array; detection limit is 3 pg/ml; 5000 cells per well in coculture.

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