Figure 3

Expression of membrane-associated prostaglandin E synthase-1 (mPGES-1) and cyclooxygenase-2 (COX-2) mRNA in cytokine-stimulated chondrocytes. Chondrocytes from patients with osteoarthritis were incubated with interleukin-1β (IL-1β; 0.01–10 ng/ml) or tumor necrosis factor-α (TNF-α; 0.01–10 ng/ml) for 12 h. Total RNA from the cells was subjected to Northern blot analysis for mPGES-1, COX-2, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), as described in Materials and methods. Representative results from three patients are shown.