Figure 8
Effect of MK-886 on endogenous prostaglandin E2 (PGE2) production in chondrocytes stimulated with IL-1β (IL-1β). Chondrocytes from patients with osteoarthritis were incubated with IL-1β in the presence of MK-886 (1 or 10 μM) for 48 h. The PGE2 concentration of the medium was then measured by enzyme-linked immunosorbent assay and calculated as a percentage of the control value (IL-1β alone). Data are means ± SD for triplicate experiments; representative results from three patients are shown. #P < 0.01, untreated control compared with IL-1β alone; *P < 0.01, IL-1β alone compared with IL-1β plus MK-886.