Membrane-associated prostaglandin E synthase-1 is upregulated by proinflammatory cytokines in chondrocytes from patients with osteoarthritis

Table 1 Oligonucleotide primers used in the reverse transcription-polymerase chain reaction

Target gene encoding	Product length (bp)	Primer (5' → 3')^a	Cycle
Type I collagen	614	CAA CGG TGC TCC TGG TGA AG GCT GGT CAG CCC TGT AGA AG	32
Type II collagen	507	TCA ACA ACC AGA TTG AGA GCA ACG TGA ACC TGC TAT TGC CCT	32
mPGES-1	459	ATG CCT GCC CAC AGC CTG GT TCA CAG GTG GCG GGC CGC TT	26
mPGES-2	502	GCA GCT GAC CCT GTA CCA GT CTC GCG GAC AAT GTA GTC AA	32
cPGES	485	ATG CAG CCT GCT TCT GCA AA CCT TAC TCC AGA TCT GGC AT	30
COX-1	401	CTT TCT CCA ACG TGA GCT ATT GTG CAT CAA CAC AGG CGC CTC TTC	32
COX-2	1157	AGA CAG ATC ATA AGC GAG GGC C ACT TGC ATT GAT GGT GAC TGT	28
GAPDH	598	CCA CCC ATG GCA AAT TCC ATG GCA TCT AGA CGG CAG GTC AGG TCC ACC	26

COX, cyclooxygenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mPGES, membrane-associated prostaglandin E synthase; cPGES, cytosolic prostaglandin E synthase. ^aFor each target gene, the upper primer is the forward primer and the lower primer is the reverse primer.

ISSN: 1478-6362