Skip to main content

Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Table 1 Oligonucleotide primers used in the reverse transcription-polymerase chain reaction

From: Membrane-associated prostaglandin E synthase-1 is upregulated by proinflammatory cytokines in chondrocytes from patients with osteoarthritis

Target gene encoding Product length (bp) Primer (5' → 3')a Cycle
Type I collagen 614 CAA CGG TGC TCC TGG TGA AG
GCT GGT CAG CCC TGT AGA AG
32
Type II collagen 507 TCA ACA ACC AGA TTG AGA GCA
ACG TGA ACC TGC TAT TGC CCT
32
mPGES-1 459 ATG CCT GCC CAC AGC CTG GT
TCA CAG GTG GCG GGC CGC TT
26
mPGES-2 502 GCA GCT GAC CCT GTA CCA GT
CTC GCG GAC AAT GTA GTC AA
32
cPGES 485 ATG CAG CCT GCT TCT GCA AA
CCT TAC TCC AGA TCT GGC AT
30
COX-1 401 CTT TCT CCA ACG TGA GCT ATT
GTG CAT CAA CAC AGG CGC CTC TTC
32
COX-2 1157 AGA CAG ATC ATA AGC GAG GGC C
ACT TGC ATT GAT GGT GAC TGT
28
GAPDH 598 CCA CCC ATG GCA AAT TCC ATG GCA
TCT AGA CGG CAG GTC AGG TCC ACC
26
  1. COX, cyclooxygenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mPGES, membrane-associated prostaglandin E synthase; cPGES, cytosolic prostaglandin E synthase. aFor each target gene, the upper primer is the forward primer and the lower primer is the reverse primer.