Figure 2
The effects of 1α,25-hydroxyvitamin D3 (1,25) on matrix metalloproteinase (MMP)-1, MMP-3 and MMP-9 production by rheumatoid synovial fibroblasts (RSFs) after 48 h incubation. (a) MMP-1 production by RSFs (n = 3) showing normalized values for control; + 1,25 (10-8 mol/l); + interleukin (IL)-1β (0.05 ng/ml); and + IL-1β and 1,25 (0.05 ng/ml and 10-8 mol/l, respectively). Before normalization, control values for MMP-1 were in the range 50–200 ng/ml culture medium/106 cells per 48 h. (b) MMP-3 production by RSF (n = 3) showing normalized values for control; + 1,25; + IL-1β; and + IL-1β and 1,25. Before normalization, control values for MMP-3 were in the range 10–40 ng/ml culture medium/106 cells per 48 h. (c) MMP-9 production by RSFs (n = 3) showing normalized values for control; + 1,25; + IL-1β; and +IL-1β and 1,25. Before normalization, control values for MMP-9 were in the range 10-50 ng/ml culture medium/106 cells per 48 h. Values are shown as means ± SEM.