Figure 1

Cytokine and activation marker expressions by CD4⁺CD45RO⁺T cells from RA synovial tissue (RA1). RA synovial tissue CD4⁺CD45RO⁺ Tcells were sorted, and cytokine and activation marker expressions were immediately determined in the TCR Cβ (TCRBC) mRNA-positive wells employing single-cell RT-PCR. Amplified PCR products were separated by electrophoresis through 2.0% agarose. As negative controls, one well that did not contain a cell (C1) and one well that was not prepared with reverse transcriptase (C2) were analyzed.