Table 3 Primers used for PCR amplification
Cytokine/chemokine receptor Primer | |
|---|---|
IL-2 | 5'-TAC AAG AAT CCC AAA CTC ACC AGG A |
3'-GTC AGT GTT GAG ATG ATG CTT TGA CA | |
3' (nested)-TGG TTG CTG TCT CAT CAG CAT ATT CA | |
IL-4 | 5'-CTT TGC TGC CTC CAA GAA CAC AAC T |
3'-TCT CAT GAT CGT CTT TAG CCT TTC CA | |
3' (nested)-TCC TTC ACA GGA CAG GAA TTC AAG C | |
IL-6 | 5'-GTT CCT GCA GAA AAA GGC AAA G |
3'-CTG AGG TGC CCA TGC TAC ATT T | |
5' (nested)-CTA GAT GCA ATA ACC ACC CCT GA | |
IL-10 | 5'-TTA GGA AGA GAA ACC AGG GAG CC |
3'-GCT GGC CAC AGC TTT CAA GAA T | |
5' (nested)-CAG GGA GCC CCT TTG ATG ATT A | |
IL-13 | 5'-ACC CAG GGA TGA CAT GTC CCT A |
3'-CCC AAC GGT GAC AAA CAC ACT C | |
3' (nested)-AAG AAG TGT GCC TGA GCT GCT GT | |
IFN-γ | 5'-AAG GCT TTA TCT CAG GGG CCA ACT |
3'-TGG AAG CAC CAG GCA TGA AAT CTC | |
5' (nested)-CAA GAT CCC ATG GGT TGT GTG TTT | |
TNF-α | 5'-GGC TCA GAC ATG TTT TCC GTG A |
3'-CTC AGC AAT GAG TGA CAG TTG G | |
5' (nested)-CAA TAG GCT GTT CCC ATG TAG CC | |
LT-α | 5'-CCT GAA CCA TCC CTG ATG TCT G |
3'-AAA TAG TCC CCT CCC TGC CTC T | |
3' (nested)-TCT AGT CAT CCC CCA AGC TCC TC | |
CD154 | 5'-AGT CAG GCC GTT GCT AGT CAG T |
3'-GGA AAC AAT GGA GAC TGC AGG TA | |
3' (nested)-TAA TGA GGA GTG GGC AGG CTC AG | |
TRANCE | 5'-GAA AAC TTG CAG CTA AGG AGG GG |
3'-GGT GGC CAA CAT CCT GCT TAT T | |
3' (nested)-CCT GCT TAT TAT TCA AGG CAT CC | |
CCR1 | 5'-GAT TTG GGC TCT TGG AAT CCT G |
3'-GTG CTT AGC CCA CTC CCT GAA T | |
3' (nested)-AGG GCT TTC TTA GTT CCA CTG CC | |
CCR2 | 5'-TGG GAG TTT TGG TGG AGT CCG AT |
3'-GGG GGA TGT GGC CTA AGA AGC AT | |
3' (nested)-GCC TAA GAA GCA TCT GAA CAA TGG | |
CCR3 | 5'-CTA AGG TCA TTA CCA CAG GCC AGG |
3'-AGC AGG GAA AGA ACT AGG CAC ATT | |
5' (nested)-GCA GCG TAC TCA TCA TCA ACC C | |
CCR5 | 5'-CTC AGG GAA TGA AGG TGT CAG A |
3'-TGC TAC TGT TGC ACT CTC CAC AAC T | |
5' (nested)-AGC CTC TGA ATA TGA ACG GTG AGC | |
CCR6 | 5'-CAT GGA ACT CAT GTT TTT AAA GGG C |
3'-CCA TGC CTA GCC CAT GAC AGT A | |
3' (nested)-CCC ATG ACA GTA CCT TCC TAA CA | |
CCR7 | 5'-AGC ACA CTC ATC CCC TCA CTT G |
3'-AGC CAA GAG CTG AGT GCA TGT C | |
3' (nested)-GAG TGC ATG TCA TCC CCA CTC T | |
CXCR1 | 5'-CAG ATC TAT GCC ACA AGA ACC CC |
3'-CTT TCT AGG GAT GCT GAT GCT GC | |
3' (nested)-CTG ATG CTG CAC GCC AGC CTG GA | |
CXCR2 | 5'-ATT ACC AGG GAC TGA GGG GAG GG |
3'-GTG GCA TTA AGT CAC ATT GCG G | |
3' (nested)-GTC ACA TTG CGG TAC AAC TAT CAC | |
CXCR3 | 5'-CAC TGC CCT TCT CAT TTG GAA ACT |
3'-GCA AAT ATA GAG GTC TTG GGG AC | |
5' (nested)-AGT ACA AGG CAT GGC GTA GAG GG | |
CXCR4 | 5'-GGA CCT GTG GCC AAG TTC TTA GTT |
3'-ACT GTA GGT GCT GAA ATC AAC CCA | |
3' (nested)-CAG CTG GGG ATC ATT TCT AGC TTT | |
CXCR5 | 5'-CAG GAC AAC GAG AAA GCC CTA AG |
3'-GGT CTC TGT GCT GCC TGT ACT GT | |
5' (nested)-GTA TCT CCT CGC AAG CTG GGT AA | |
TCR Cβ | 5'-TCA AGT CCA GTT CTA CGG GCT C |
3'-TCA TAG AGG ATG GTG GCA GAC A | |
5' (nested)-CTC TCG GAG AAT GAC GAG TGG AC | |