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Figure 2 | Arthritis Res Ther

Figure 2

From: Pharmacological disruption of insulin-like growth factor 1 binding to IGF-binding proteins restores anabolic responses in human osteoarthritic chondrocytes

Figure 2

Western ligand blotting analysis of insulin-like growth factor (IGF)-binding proteins (IGFBPs) secreted by human osteoarthritis (OA) chondrocytes or human OA cartilage. Aliquots of the 48-hour secretion medium of confluent OA chondrocytes or cartilage explants were electrophoresed and electroblotted onto a nitrocellulose membrane. IGFBPs were detected by incubating the membrane with a mixture of 125I-labeled insulin-like growth factor (IGF)-1 and 125I-labeled IGF-2, followed by autoradiography. Shown are two and three representative specimens of IGFBP-containing media from chondrocytes and cartilage explants, respectively.

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