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Figure 6 | Arthritis Res Ther

Figure 6

From: Pharmacological disruption of insulin-like growth factor 1 binding to IGF-binding proteins restores anabolic responses in human osteoarthritic chondrocytes

Figure 6

Effect of NBI-31772, a small-molecule inhibitor of the binding of insulin-like growth factor (IGF)-1 to IGF-binding proteins, on 125I-labeled IGF-1 binding to human osteoarthritic chondrocytes. Confluent chondrocytes were incubated with 1 × 105 dpm 125I-labeled IGF-1 with or without excess (150 nM) IGF-1 or 10 μM NBI-31772 for 1 hour at 4°C. Medium was harvested and cells were washed three times with Dulbecco's phosphate-buffered saline. Cells were lysed in 1 N NaOH. The percentage of 125I-labeled IGF-1 bound to the cells was calculated as dpm in NaOH-treated cells over total dpm. Data are expressed as the means and SEM of four replicates from a representative experiment (out of two independent cultures).

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