Table 2 Sequences of primers used for estrogen receptor-α genotyping
Polymorphism site | Primer sets | Annealing temperature (°C) | Restriction enzyme | Allele size (bp) |
|---|---|---|---|---|
Intron 1 T/C | 5'-ctgccaccctatctgtatcttttcctattctcc-3' 5'-tctttctctgccaccctggcgtcgattatctga-3' | 64 | PvuII | T: 936 + 438 C: 1374 |
Intron 1 A/G | 5'-ctgccaccctatctgtatcttttcctattctcc-3' 5'-tctttctctgccaccctggcgtcgattatctga-3' | 64 | XbaI | A: 981 + 393 G: 1374 |
Exon 8 G/A | 5'-gaggagacggaccaaagccac-3' 5'-gccattggtgttggatgcatg-3' | 63 | BtgI | G: 129 + 98 A: 227 |