Table 2 Sequences of primers used for estrogen receptor-α genotyping
| Polymorphism site | Primer sets | Annealing temperature (°C) | Restriction enzyme | Allele size (bp) |
|---|---|---|---|---|
| Intron 1 T/C |
5'-ctgccaccctatctgtatcttttcctattctcc-3' 5'-tctttctctgccaccctggcgtcgattatctga-3' | 64 | PvuII |
T: 936 + 438 C: 1374 |
| Intron 1 A/G |
5'-ctgccaccctatctgtatcttttcctattctcc-3' 5'-tctttctctgccaccctggcgtcgattatctga-3' | 64 | XbaI |
A: 981 + 393 G: 1374 |
| Exon 8 G/A |
5'-gaggagacggaccaaagccac-3' 5'-gccattggtgttggatgcatg-3' | 63 | BtgI |
G: 129 + 98 A: 227 |