Skip to main content

Table 2 Sequences of primers used for estrogen receptor-α genotyping

From: Estrogen receptor-α gene haplotype is associated with primary knee osteoarthritis in Korean population

Polymorphism site Primer sets Annealing temperature (°C) Restriction enzyme Allele size (bp)
Intron 1 T/C 5'-ctgccaccctatctgtatcttttcctattctcc-3'
5'-tctttctctgccaccctggcgtcgattatctga-3'
64 PvuII T: 936 + 438
C: 1374
Intron 1 A/G 5'-ctgccaccctatctgtatcttttcctattctcc-3'
5'-tctttctctgccaccctggcgtcgattatctga-3'
64 XbaI A: 981 + 393
G: 1374
Exon 8 G/A 5'-gaggagacggaccaaagccac-3'
5'-gccattggtgttggatgcatg-3'
63 BtgI G: 129 + 98
A: 227
  1. bp, base pairs.