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Figure 1 | Arthritis Res Ther

Figure 1

From: Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers

Figure 1

Fluorescence activated cell sorting analysis of fresh isolated chondrocytes from osteoarthritic cartilage. (a) Forward/side scatter. (b) Markers were set in the channel display with a maximum of 2% positive cells by staining with isotype control antibody fluorescein isothiocyanate (FITC)-conjugated mouse IgG1. (c-e) Triple staining experiments for CD9-FITC/CD90-allophycocyanine (APC)/CD166- phycoerythrin (PE). Panel c shows a histogram of FL1 CD9-FITC. Based on isotype and histogram, cells were divided into positive or negative: panel d, CD9-, double-stained CD90-APC/CD166-PE; and panel e, CD9+, double-stained CD90-APC/CD166-PE.

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Arthritis Research & Therapy

ISSN: 1478-6362

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