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Table 2 Interferon-γ production by cytotoxic T lymphocyte lines after incubation on anti-hamster IgG or anti-CD3 in the presence or absence of Lip-OspA, Met-Asp-Pro-OspA, recombinant interleukin-2 or lipopolysaccharide

From: Direct Toll-like receptor 2 mediated co-stimulation of T cells in the mouse system as a basis for chronic inflammatory joint disease

Addition B6 anti-BALB/c, ng/ml (SI) TLR-2-/- anti-BALB/c, ng/ml (SI)
Anti-haIgG 0.2 0.3
   + Lip-OspA 2.3a (11.5) 0.2 (<1)
   + MDP-OspA 0.3 (1.5) 0.2 (<1)
   + rec. IL-2 0.5 (2.5) 0.4 (1.3)
   + LPS 0.2 (1.0) 0.4 (1.3)
0.03 ng per well anti-CD3 0.2 0.3
   + anti-CD3 + Lip-OspA 5.4a (27) 0.2 (<1)
   + anti-CD3 + MDP-OspA 0.2 (1.0) 0.3 (1.0)
   + anti-CD3 + rec. IL-2 0.8a (4.0) 0.6 (2.0)
   + anti-CD3 + LPS 0.3 (1.5) 0.1 (<1)
0.3 ng per well anti-CD3 4.9 7.2
   + anti-CD3 + Lip-OspA 29.1a (5.9) 6.8 (<1)
   + anti-CD3 + MDP-OspA 6.0 (1.2) 9.5 (1.3)
   + anti-CD3 + rec. IL-2 10.9a (2.2) 10.2 (1.4)
   + anti-CD3 + LPS 2.7 (<1) 7.2 (1.0)
  1. aSignificant difference (P < 0.05) from control (anti-haIgG or anti-CD3 without supplements). C57BL/6 (B6) and Toll-like receptor (TLR)-2-/- cytotoxic T lymphocyte lines (generated against BALB/c, fourth stimulation, day 4) were incubated for 6 h on anti-haIgG (control) with or without anti-CD3 (0.03 ng per well or 0.3 ng per well) in the presence or absence of Lip-OspA, Met-Asp-Pro (MDP)-OspA (10 μg/ml each), recombinant interleukin-2 (rec. Il-2; 50 U/ml) or lipopolysaccharide (LPS; 1 μg/ml). The amount of the secreted interferon-γ in the supernatant was then tested in duplicate using the enzyme-linked immunosorbent assay technique. One representative experiment is shown. The detection limit was 0.1 ng/ml. SI, stimulation index (calculated based on results with anti-hamster (ha)IgG plus anti-CD3 or anti-haIgG alone, without the addition of supplements).