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Table 3 Expression of TLRs on resting and activated T cells in comparison with macrophages and B cells

From: Direct Toll-like receptor 2 mediated co-stimulation of T cells in the mouse system as a basis for chronic inflammatory joint disease

Cell population Molecules per cell
  TLR-1 TLR-2 TLR-4 TLR-6
Spleen Thy1.2+ (ex vivo) 611 46 n.d. n.d.
Spleen Thy1.2+ + PMA/ionomycin 40 24 6 n.d.
Spleen CD4+ T cells 1874 30 7 47
Spleen CD8+ T cells 602 31 0 39
CTLs 248 219 43 56
CTLs + PMA/ionomycin 19 335 53 19
CTLs (TLR-2-/-) 0 -a 28 6
Spleen (TLR-2-/-, ex vivo, unselected) 101 -a 78 18
Bone marrow macrophages (cultured) -b 6375 7056 113
Spleen mature B cells -b 25 25 13
Spleen marginal-zone B cells -b 49 33 11
  1. aNot determined; sterile fusion transcripts of the mutated Toll-like receptor (TLR)-2 gene can be found with the indicated primer pairs; however, no protein product is detectable (CJ Kirschning, unpublished observations). bNot determined. Purified T cells (Thy1.2+, CD4+ or CD8+) or B cells (mature, marginal zone) from B6 mice, whole splenocytes from TLR-2-/- mice or purified cytotoxic T lymphocytes (CTLs) from C57BL/6 and TLR-2-/- anti-BALB/c mixed lymphocyte culture (purified by cell sorting for CD8-positive cells) were stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin for 24 h or frozen directly in TriReagent for RNA isolation and real-time polymerase chain reaction, as described in Materials and methods. As a control, cultured bone marrow-derived macrophages were used. Experiments, except for the measurement of mRNA in CTL lines and spleen cells that were stimulated with PMA and ionomycin, were repeated twice and gave similar results, both in the sense of inter-experimental and intra-experimental reproducibilities. n.d., not detectable.